Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,885,724 A→T 7.4% L52Q (CTG→CAG)  PP_2541 ← transcriptional factor‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,885,7240AT 7.4% 157.9 / 2.3 54L52Q (CTG→CAG) PP_2541transcriptional factor‑like protein
Reads supporting (aligned to +/- strand):  ref base A (29/21);  new base T (2/2);  total (31/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

GCATAAAGTACGACGTGTGTCGGTGGGGTAAGTGCGTCGGGTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCCACTGGCGGGCGATGGTGTGGCGGGTCGGGT  >  NC_002947/2885577‑2885868
                                                                                                                                                   |                                                                                                                                                
gCATAAAGTACGACGTGTGTCGGTGGGGTAAGTGCGTCGGGTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAg                                                                                                                                                 >  4:252377/1‑149 (MQ=255)
  aTAAAGTACGACGTGTGTCGGTGGGGTAAGTGCGTCGGGTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGt                                                                                                                                               >  7:142282/1‑149 (MQ=255)
  aTAAAGTACGACGTGTGTCGGTGGGGTAAGTGCGTCGGGTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGt                                                                                                                                               >  2:181512/1‑149 (MQ=255)
          cgacgTGTGTCGGTGGGGTAAGTGCGTCGGGTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTc                                                                                                                                        >  6:127740/1‑148 (MQ=255)
          cgacgTGTGTCGGTGGGGTAAGTGCGTCGGGTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTc                                                                                                                                        >  1:322352/1‑148 (MQ=255)
                   tCGGTGGGGTAAGTGCGTCGGGTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGGCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGt                                                                                                                              >  8:24955/1‑149 (MQ=255)
                   tCGGTGGGGTAAGTGCGTCGGGGTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGt                                                                                                                              >  7:204336/1‑149 (MQ=255)
                         gggTAAGTGCGTCGGGTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCt                                                                                                                         <  5:127740/148‑1 (MQ=255)
                           gTAAGTGCGTCGGGTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCtggt                                                                                                                      >  1:81586/1‑149 (MQ=255)
                            tAAGTGCGTCGGGTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCtggtg                                                                                                                     >  5:329500/1‑149 (MQ=255)
                            tAAGTGCGTCGGGTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCtggtg                                                                                                                     <  3:155412/149‑1 (MQ=255)
                                  cGTCGGGTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATg                                                                                                                <  8:142282/148‑1 (MQ=255)
                                   gTCGGGTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGc                                                                                                              <  3:252377/149‑1 (MQ=255)
                                        gTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACg                                                                                                         >  4:277867/1‑149 (MQ=255)
                                        gTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACg                                                                                                         >  7:232331/1‑149 (MQ=255)
                                         ttGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACgg                                                                                                        <  4:96859/149‑1 (MQ=255)
                                            cAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGtt                                                                                                      <  8:204336/148‑1 (MQ=255)
                                              gctgctGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTc                                                                                                   <  2:81586/149‑1 (MQ=255)
                                               ctgctgCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGt                                                                                                    >  4:191212/1‑147 (MQ=255)
                                                tgctgcGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAg                                                                                                 >  1:323817/1‑149 (MQ=255)
                                                 gctgcGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGt                                                                                                                                  <  6:71206/115‑1 (MQ=255)
                                                 gctgcGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGt                                                                                                                                  >  5:71206/1‑115 (MQ=255)
                                                      gcTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGc                                                                                           <  6:329500/149‑1 (MQ=255)
                                                           gCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGc                                                                                       >  2:267249/1‑148 (MQ=255)
                                                            cccAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCa                                                                                                  <  1:127181/136‑1 (MQ=255)
                                                            cccAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCa                                                                                                  >  2:127181/1‑136 (MQ=255)
                                                             ccAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGc                                                                                                                                <  2:20442/105‑1 (MQ=255)
                                                             ccAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGc                                                                                                                                >  1:20442/1‑105 (MQ=255)
                                                             ccAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAAc                                                                                    >  2:100715/1‑149 (MQ=255)
                                                              cAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACt                                                                                   <  1:181512/149‑1 (MQ=255)
                                                                 gCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGGGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTggg                                                                                <  2:322352/149‑1 (MQ=255)
                                                                   tCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTg                                                                              >  5:239/1‑149 (MQ=255)
                                                                   tCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGGCAGGGCAGCCTTGAACTGGGTg                                                                              >  8:101524/1‑149 (MQ=255)
                                                                     aaGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTgg                                                                             <  3:191212/148‑1 (MQ=255)
                                                                     aaGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTg                                                                              >  2:164903/1‑147 (MQ=255)
                                                                     aaGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTg                                                                              <  1:100715/147‑1 (MQ=255)
                                                                     aaGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGa                                                                            <  2:323817/149‑1 (MQ=255)
                                                                                 cAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCgg                                                                <  8:232331/149‑1 (MQ=255)
                                                                                      ccAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTggc                                                           >  3:265298/1‑149 (MQ=255)
                                                                                       cAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTggcg                                                          >  2:7015/1‑149 (MQ=255)
                                                                                         gCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTTGAGCTCATTCCCGGGTggcggc                                                        <  1:1301/149‑1 (MQ=255)
                                                                                                       cTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACtt                                           >  8:287087/1‑148 (MQ=255)
                                                                                                            cTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAg                                     >  7:221025/1‑149 (MQ=255)
                                                                                                             tGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGt                                    >  5:305513/1‑149 (MQ=255)
                                                                                                               ttGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGtt                                   <  7:287087/148‑1 (MQ=255)
                                                                                                                 gCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGc                                 >  5:288378/1‑148 (MQ=255)
                                                                                                                       aaGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTggc                                                           <  2:40811/116‑1 (MQ=255)
                                                                                                                       aaGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTggc                                                           >  1:40811/1‑116 (MQ=255)
                                                                                                                       aaGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGtt                                   >  7:248628/1‑140 (MQ=255)
                                                                                                                       aaGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGtt                                   <  8:248628/140‑1 (MQ=255)
                                                                                                                                   accgtcgAGCGCGACCTGGTCGAGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCCACTGGCGGGCGATGgtgt              >  5:150973/6‑149 (MQ=255)
                                                                                                                                         gAGCGCGACCTGGTCGAGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCCACTGGCGGGCGATGGTGTGGcggg        >  2:124159/1‑149 (MQ=255)
                                                                                                                                           gcgcGACCTGGTCGAGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCCACTGGCGGGCGATGGTGTGGcgggtc      <  6:150973/146‑1 (MQ=255)
                                                                                                                                               gACCTGGTCGAGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCCACTGGCGGGCGATGGTGTGGcgggtcgggt  <  1:124159/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GCATAAAGTACGACGTGTGTCGGTGGGGTAAGTGCGTCGGGTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCCACTGGCGGGCGATGGTGTGGCGGGTCGGGT  >  NC_002947/2885577‑2885868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑