Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,946,588 G→C 22.5% P312A (CCG→GCG) ‡ yijP ← phosphoethanolamine transferase CptA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,946,5880GC22.5% 58.5 / 13.4 31P312A (CCG→GCG) ‡yijPphosphoethanolamine transferase CptA
Reads supporting (aligned to +/- strand):  ref base G (15/9);  new base C (2/5);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 1.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGCGCTGGTTGTTCAGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAGCGCGTCGAGGTTGGGCGTG  >  NC_002947/2946445‑2946736
                                                                                                                                               |                                                                                                                                                    
gTTGCGCTGGTTGTTCAGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGc                                                                                                                                                 >  6:215655/1‑149 (MQ=255)
    cgcTGGTTGTTCAGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACCCGTCGCTGat                                                                                                                                             <  7:32730/149‑2 (MQ=255)
    cgcTGGTTGTTCAGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACCCGTCGCTGa                                                                                                                                              <  4:302037/148‑1 (MQ=255)
        ggTTGTTCAGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACCCGTCGCTGatcaac                                                                                                                                         <  7:118180/149‑6 (MQ=255)
        ggTTGTTCAGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACCCGTCGCTGatcaa                                                                                                                                          >  3:304339/1‑144 (MQ=255)
        ggTTGTTCAGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACCCGTCGCTGatcaa                                                                                                                                          <  4:304339/148‑5 (MQ=255)
                      acCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTg                                                                                                                           <  2:244095/149‑1 (MQ=255)
                                        tgcGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAg                                                                                                         >  4:140141/1‑149 (MQ=255)
                                          cGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGa                                                                                                       >  3:250169/1‑149 (MQ=255)
                                                         cATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACtt                                                                                        >  6:94338/1‑149 (MQ=255)
                                                             gtgtTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGa                                                                                     <  5:215655/148‑1 (MQ=255)
                                                              tgtTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATg                                                                                   <  3:140141/149‑1 (MQ=255)
                                                                ttGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGgt                                                                                 >  3:210063/1‑149 (MQ=255)
                                                                          tCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGgcgc                                                                       >  4:90727/1‑149 (MQ=255)
                                                                                      ggttggtGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACg                                                           >  5:183325/1‑149 (MQ=255)
                                                                                           gtGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTcgcg                                                      >  8:282460/1‑149 (MQ=255)
                                                                                             gATCCAGAAGGTCTTGAAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGa                                                    >  1:117448/1‑149 (MQ=255)
                                                                                                 cAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAacac                                                <  5:94338/149‑1 (MQ=255)
                                                                                                 cAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAacac                                                >  8:218418/1‑149 (MQ=255)
                                                                                                 cAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAaca                                                 <  7:282460/148‑1 (MQ=255)
                                                                                                          ttGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAACCGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGct                                       >  5:78809/1‑149 (MQ=255)
                                                                                                          ttGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAAAGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGct                                       >  6:334706/1‑149 (MQ=255)
                                                                                                            gTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTGGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGctct                                     >  7:54155/1‑149 (MQ=255)
                                                                                                               gcctgcctGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTg                                   >  3:246347/1‑148 (MQ=255)
                                                                                                               gcctgcctGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGt                                  <  4:246347/149‑1 (MQ=255)
                                                                                                                  tgcctgTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGc                               >  2:13594/1‑149 (MQ=255)
                                                                                                                     ctgTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTgg                            <  3:90727/149‑1 (MQ=255)
                                                                                                                        tttCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTggcg                          <  4:250169/148‑1 (MQ=255)
                                                                                                                                     ctCAGCGACCCGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAac                                                  <  4:114107/110‑1 (MQ=255)
                                                                                                                                     ctCAGCGACCCGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAac                                                  >  3:114107/2‑111 (MQ=255)
                                                                                                                                      tCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAGCGCGTCGAg            <  4:210063/148‑1 (MQ=255)
                                                                                                                                               ggTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAGCGCGTCGAGGTTGGGCgtg  <  6:183325/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
GTTGCGCTGGTTGTTCAGGTACACCGGCGCGTCTGTTTGCTGCGAGAAGGTGGTGAGCATGGTGTTGCGCTTGGTCAAGGTCTGCTGGTTGGTGATCCAGAAGGTCTTGTAGCCTGCCTGTTTCAGCAGGTTGATCAGCGACGGGTCGCTGAGGAAACGGTCCGGGTTCTGCTCGTCGCCGAACGTGAGGATCTGCTGCATCACTTCGATGGTGTACGGGCGCGGCGATACCACGTCGCGGAACACGGTCAGGCTCTTGTCGCTGGCGGCCAGCGCGTCGAGGTTGGGCGTG  >  NC_002947/2946445‑2946736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑