Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,964,888 G→C 6.1% P580R (CCG→CGG)  PP_2595 ← ABC transporter permease/ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,964,8880GC 6.1% 211.9 / 3.7 66P580R (CCG→CGG) PP_2595ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (30/32);  new base C (2/2);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.94e-01

GGCTTGCGGTCAGGGTCAGCCAGGCGTCGCCTTGCAGGGTAAGCAACTGAGACCAGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACG  >  NC_002947/2964740‑2965034
                                                                                                                                                    |                                                                                                                                                  
ggcTTGCGGTCAGGGTCAGCCAGGCGTCGCCTTGCAGGGTAAGCAACTGAGACCAGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCg                                                                                                                                                    <  8:166984/149‑1 (MQ=255)
                    cAGGCGTCGCCTTGCAGGGTAAGCAACTGAGACCAGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCg                                                                                                                                >  8:158592/1‑149 (MQ=255)
                      ggCGTCGCCTTGCAGGGTAAGCAACTGAGACCAGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGtt                                                                                                                              <  6:140421/149‑1 (MQ=255)
                         gTCGCCTTGCAGGGTAAGCAACTGAGACCAGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGt                                                                                                                                                  >  3:237499/1‑126 (MQ=255)
                         gTCGCCTTGCAGGGTAAGCAACTGAGACCAGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGt                                                                                                                                                  <  4:237499/126‑1 (MQ=255)
                              cTTGCAGGGTAAGCAACTGAGACCAGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGt                                                                                                                      <  7:158592/149‑1 (MQ=255)
                              cTTGCACGGTAAGCAACTGAGACCAGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGAGCCAGCCGTTGGCGGCGt                                                                                                                      >  8:187858/1‑149 (MQ=255)
                                    gggTAAGCAACTGAGACCAGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCt                                                                                                                <  6:121727/149‑1 (MQ=255)
                                     ggTAAGCAACTGAGACCAGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTg                                                                                                               >  5:203846/1‑149 (MQ=255)
                                     ggTAAGCAACTGAGACCAGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCCGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTg                                                                                                               >  6:84030/1‑149 (MQ=255)
                                     ggTAAGCAACTGAGACCAGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCCGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTg                                                                                                               >  6:79329/1‑149 (MQ=255)
                                      gTAAGCAACTGAGACCAGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGt                                                                                                              <  5:120448/149‑1 (MQ=255)
                                       tAAGCAACTGAGACCAGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGtt                                                                                                             <  2:302584/149‑1 (MQ=255)
                                          gCAACTGAGACCAGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGa                                                                                                          <  5:267468/149‑1 (MQ=255)
                                           cAACTGAGACCAGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAg                                                                                                         >  6:110571/1‑149 (MQ=255)
                                             aCTGAGACCAGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGc                                                                                                        >  5:52831/1‑148 (MQ=255)
                                             aCTGAGACCAGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGc                                                                                                        <  6:52831/148‑1 (MQ=255)
                                                 agaCCAGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCg                                                                                                   >  5:63509/1‑149 (MQ=255)
                                                      aGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGAGGCGTGGCGCTGTTCGAGCATGCGTGCa                                                                                               >  6:65733/1‑148 (MQ=255)
                                                       ggCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATa                                                                                             <  5:65733/149‑1 (MQ=255)
                                                        gCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAg                                                                                            <  8:3678/149‑1 (MQ=255)
                                                               cACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGc                                                                                     >  3:46087/1‑149 (MQ=255)
                                                               cACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGc                                                                                     >  4:306394/1‑149 (MQ=255)
                                                                aCGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGc                                                                                     >  6:188977/1‑148 (MQ=255)
                                                                aCGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGc                                                                                     >  6:279808/1‑148 (MQ=255)
                                                                aCGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGc                                                                                     >  8:242944/1‑148 (MQ=255)
                                                                    aCGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTg                                                                                >  2:83725/1‑149 (MQ=255)
                                                                    aCGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTg                                                                                <  2:74436/149‑1 (MQ=255)
                                                                      gCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGgc                                                                              <  5:279808/149‑1 (MQ=255)
                                                                      gCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGgc                                                                              >  2:71704/1‑149 (MQ=255)
                                                                        cGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGgcgc                                                                            <  6:63509/149‑1 (MQ=255)
                                                                        cGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGgcgc                                                                            >  6:207862/1‑149 (MQ=255)
                                                                            aGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAg                                                                        >  1:301288/1‑149 (MQ=255)
                                                                              ccAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTggc                                                                                                                           <  1:301546/96‑1 (MQ=255)
                                                                              ccAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTggc                                                                                                                           <  1:306494/96‑1 (MQ=255)
                                                                              ccAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTggc                                                                                                                           >  2:301546/1‑96 (MQ=255)
                                                                              ccAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTggc                                                                                                                           >  2:306494/1‑96 (MQ=255)
                                                                               cAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAg                                                                        >  8:151163/1‑146 (MQ=255)
                                                                               cAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAg                                                                        <  7:151163/146‑1 (MQ=255)
                                                                                aGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGtgtg                                                                    <  5:207862/149‑1 (MQ=255)
                                                                                    aGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCAGTTCGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCg                                                                >  6:277650/1‑149 (MQ=255)
                                                                                    aGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGGCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCg                                                                <  6:304814/149‑1 (MQ=255)
                                                                                    aGGCCGCGCACAACACCAGGAACAGGAAGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCg                                                                >  1:177274/1‑149 (MQ=255)
                                                                                        cgcgCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCCGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGgtgt                                                            <  5:84030/149‑1 (MQ=255)
                                                                                        cgcgCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCCGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGgtgt                                                            <  5:79329/149‑1 (MQ=255)
                                                                                          cgcACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGc                                                          <  2:90924/149‑1 (MQ=255)
                                                                                          cgcACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGc                                                          <  1:71704/149‑1 (MQ=255)
                                                                                          cgcACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGc                                                          <  1:83725/149‑1 (MQ=255)
                                                                                          cgcACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGc                                                          <  4:46087/149‑1 (MQ=255)
                                                                                          cgcACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGGGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGc                                                          <  4:201010/149‑1 (MQ=255)
                                                                                             acaacaCCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCtt                                                       <  5:110571/149‑1 (MQ=255)
                                                                                             acaacaCCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCtt                                                       <  6:203846/149‑1 (MQ=255)
                                                                                                         aCAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCt                                           >  4:75172/1‑149 (MQ=255)
                                                                                                              aTGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCg                                      <  2:177274/149‑1 (MQ=255)
                                                                                                                   gCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTc                                 >  2:192110/1‑149 (MQ=255)
                                                                                                                     aTGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCa                               <  7:242944/149‑1 (MQ=255)
                                                                                                                      tGCAACACGCATAAGTCGTGTTCCACCCTCGGTTCCCCGATGCGCCCGCCGTTGGCGGCGTGGCGCTGTACGAGCATGAGTGCATAGTGCCCGCCATTGCCGAGCAGTGTGGCAGGTGTGCCTTGTTCGACAACCTGCCCGGCGTcacg                              >  8:14283/1‑146 (MQ=255)
                                                                                                                         aaCACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCa                                                                         <  4:108841/103‑1 (MQ=255)
                                                                                                                         aaCACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCa                                                                         >  3:108841/1‑103 (MQ=255)
                                                                                                                              gCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGa                      >  8:118117/1‑149 (MQ=255)
                                                                                                                                aTCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGata                    >  6:300878/1‑149 (MQ=255)
                                                                                                                                     tCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGa                                                                                                          >  6:267282/1‑58 (MQ=255)
                                                                                                                                     tCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGa                                                                                                          <  5:267282/58‑1 (MQ=255)
                                                                                                                                       gtgtTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGtt                                                    >  2:33563/1‑110 (MQ=255)
                                                                                                                                       gtgtTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGtt                                                    <  1:33563/110‑1 (MQ=255)
                                                                                                                                             cACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAAGCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATg        <  5:277650/148‑1 (MQ=255)
                                                                                                                                             cACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATg        <  7:118117/148‑1 (MQ=255)
                                                                                                                                                    ggTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACg  <  3:306394/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGCTTGCGGTCAGGGTCAGCCAGGCGTCGCCTTGCAGGGTAAGCAACTGAGACCAGGCCAGCTCACGAACGCCGACAGCCAGTGAGGCCGCGCACAACACCAGGAACAGGATGAGGCATGCAACACGCATCAGTCGTGTTCCACCCTCGGTGCCCCGATGCGCCAGCCGTTGGCGGCGTGGCGCTGTTCGAGCATGCGTGCATAGTGCCCGCCATTGGCGCGCAGTGTGGCCGGTGTGCCTTGTTCGACAACCTGCCCGGCGTCCAGCACCACGATATGGTCGGCCATGGCCACG  >  NC_002947/2964740‑2965034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑