Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,000,772 A→C 6.9% V75G (GTG→GGG)  PP_2624 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,000,7720AC 6.9% 173.7 / 4.5 58V75G (GTG→GGG) PP_2624hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (26/28);  new base C (2/2);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01

GACCAGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCTCC  >  NC_002947/3000624‑3000909
                                                                                                                                                    |                                                                                                                                         
gACCAGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCa                                                                                                                                           >  3:173811/1‑149 (MQ=255)
    aGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCATGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTc                                                                                                                                       >  1:110361/1‑149 (MQ=255)
     gTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACggc                                                                                                                                       >  1:156989/1‑146 (MQ=255)
      tGCTTCGCGCTGCGCCAGCAGGTTGTTCAGCTCGGTCACCTGGCGCGCGTGCCGGTCCGGCCCGAAATCGTTGATGGACGTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGGGGTGCCGAGCCTGGACGGTACCGTCACGTCCa                                                                                                                                     <  6:232246/149‑1 (MQ=255)
        cTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAgg                                                                                                                                   >  8:258570/1‑149 (MQ=255)
         ttCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGCACCTGAACTGCAGCTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGc                                                                                                                                  >  8:36858/1‑149 (MQ=255)
           cgcgCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGTGACGGTACCGTCCAGGc                                                                                                                                  <  3:159916/147‑1 (MQ=37)
            gcgcAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTc                                                                                                                                <  6:101330/148‑1 (MQ=255)
             cgcAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTcac                                                                                                                              <  2:92795/149‑1 (MQ=255)
              gcAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTcaca                                                                                                                             >  5:47510/1‑149 (MQ=255)
                   gcCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGc                                                                                                                        <  4:151067/149‑1 (MQ=255)
                     cagcagCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGcgt                                                                                                                      >  6:87454/1‑149 (MQ=255)
                          gcTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTc                                                                                                                 <  6:47510/149‑1 (MQ=255)
                            ttGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTc                                                                                                                 <  1:130454/147‑1 (MQ=255)
                                tCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGTGACGGTACCGTCCAgg                                                                                                                                   <  6:196656/125‑1 (MQ=255)
                                tCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGTGACGGTACCGTCCAgg                                                                                                                                   >  5:196656/1‑125 (MQ=38)
                                    cTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGt                                                                                                       <  7:258570/149‑1 (MQ=255)
                                     tCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGtt                                                                                                      >  7:214362/1‑149 (MQ=255)
                                       gggCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGATCTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGtt                                                                                                      <  3:286793/147‑1 (MQ=255)
                                       gggCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTg                                                                                                     >  4:125057/1‑148 (MQ=255)
                                         gCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACAGCGTGTTGaac                                                                                                  <  1:101206/149‑2 (MQ=255)
                                             cTGGCGCGCGATCCGGTCCGGCCCGAAATCTTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCTGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAatgtat                                                                                              <  3:195053/149‑1 (MQ=255)
                                             cTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAatgtat                                                                                              >  1:175099/1‑149 (MQ=255)
                                                  gcgcgATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTGACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGGTGAATGTATGTTTa                                                                                         >  3:170948/1‑149 (MQ=255)
                                                     cgATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATc                                                                                       >  4:266637/1‑148 (MQ=255)
                                                      gATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGtt                                                                                                      <  4:215968/132‑1 (MQ=255)
                                                      gATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGtt                                                                                                      >  3:215968/1‑132 (MQ=255)
                                                       aTCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGAt                                                                                    <  8:214362/149‑1 (MQ=255)
                                                       aTCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGAt                                                                                    >  5:59118/1‑149 (MQ=255)
                                                            gtccggCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCa                                                                               <  4:173811/149‑1 (MQ=255)
                                                             tccggCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCAt                                                                              <  5:87454/149‑1 (MQ=255)
                                                                ggCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGAc                                                                           <  3:125057/149‑1 (MQ=255)
                                                                       aaTCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACgg                                                                    <  2:175099/149‑1 (MQ=255)
                                                                             tGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGcgt                                                                                                                      <  8:140805/93‑1 (MQ=255)
                                                                             tGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGcgt                                                                                                                      >  7:140805/1‑93 (MQ=255)
                                                                                       gAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACgg                                                     >  5:187364/1‑148 (MQ=255)
                                                                                       gAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACgg                                                     <  6:187364/148‑1 (MQ=255)
                                                                                        aaCTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACAGGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACgg                                                     <  7:36858/147‑1 (MQ=255)
                                                                                           tGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCg                                                                                      <  4:57384/111‑1 (MQ=255)
                                                                                           tGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCg                                                                                      >  3:57384/1‑111 (MQ=255)
                                                                                               ggTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCAc                                                       <  2:251119/138‑1 (MQ=255)
                                                                                               ggTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCAc                                                       >  1:251119/1‑138 (MQ=255)
                                                                                                   gACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTAGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGc                                        >  8:289456/1‑149 (MQ=255)
                                                                                                      ggCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCg                                      >  6:272688/1‑148 (MQ=255)
                                                                                                        ccAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATg                                                                                   >  8:152705/1‑101 (MQ=255)
                                                                                                        ccAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATg                                                                                   <  7:152705/101‑1 (MQ=255)
                                                                                                        ccAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAg                                   >  5:262001/1‑149 (MQ=255)
                                                                                                            cTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGaa                                    <  4:269739/144‑1 (MQ=255)
                                                                                                            cTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGaa                                    >  3:269739/1‑144 (MQ=255)
                                                                                                               ggTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCAAGGCCGAAGTCGccc                             <  2:110361/148‑1 (MQ=255)
                                                                                                                                 gAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCg          >  5:144429/1‑149 (MQ=255)
                                                                                                                                   gCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGc        <  7:289456/149‑1 (MQ=255)
                                                                                                                                     cTGGACGTGACGGTACCGTCCAGGCTCGCATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAg      >  4:206920/1‑149 (MQ=18)
                                                                                                                                     cTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAg      >  3:117601/1‑149 (MQ=255)
                                                                                                                                      tGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCtt               <  7:66626/139‑1 (MQ=255)
                                                                                                                                      tGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCtt               >  8:66626/1‑139 (MQ=255)
                                                                                                                                       ggACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAgc                                                           >  2:52209/1‑94 (MQ=255)
                                                                                                                                       ggACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAgc                                                           <  1:52209/94‑1 (MQ=255)
                                                                                                                                         aCGGTACCGTCACGTCCATGCTCACATTGTCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCTcc  <  2:156989/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                         
GACCAGTGCTTCGCGCAGCGCCAGCAGCTTGTTCAGCTCGGGCACCTGGCGCGCGATCCGGTCCGGCCCGAAATCGTTGATGGACCTGAACTGCAGGTGGACGGCCAGCTCGGTATCCGGGGCGGTGCCGAGCCTGGACGGTACCGTCACGTCCAGGCTCACATTGGCGTCGTTCAACACGCTGTTGAATGTATGTTTATCGATGCGCATGACCTGACGGTCCTCAAGCGCACGGCTGTCATCCAGGCCGAAGTCGCCCAGCACCAGCATCTTGTGCGGCAGCTCC  >  NC_002947/3000624‑3000909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑