Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,049,761 C→T 8.6% V307V (GTC→GTT)  PP_2662 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,049,7610CT 8.6% 128.1 / 2.3 47V307V (GTC→GTT) PP_2662hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (23/19);  new base T (2/2);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

CACGGTAGAAACGATCACTTCCAAGGGGGTGGGTTACGGGGTGCAGGCAAAGAT‑GGGCGGCTGGTCGCTGACCGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATGGCAACGGTGCGGTTGGCAGCGGCGCGGACTACGAGACCCGGGGTGTGGCGCTGTTCCATGA  >  NC_002947/3049614‑3049907
                                                                                                                                                    |                                                                                                                                                  
cACGGTAGAAACGATCACTTCCAAGGGGGTGGGTTACGGGGTGCAGGCAAAGAT‑GGGCGGCTGGTCGCTGACCGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTc                                                                                                                                                    <  2:162603/148‑1 (MQ=255)
      aGAAACGATCACTTCCAAGGGGGTGGGTTACGGGGTGCAGGCAAAGATGGGGCGGCTGGTCGCTGACCGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGt                                                                                                                                              >  1:82495/1‑149 (MQ=255)
       gAAACGATCACTTCCAAGGGGGTGGGTTACGGGGTGCAGGCAAAGAT‑GGGCGGCTGGTCGCTGACCGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGa                                                                                                                                            <  8:85166/149‑1 (MQ=255)
          aCGATCACTTCCAAGGGGGTGGGTTACGGGGTGCAGGCAAAGAT‑GGGCGGCTGGTCGCTGACCGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATgg                                                                                                                                         <  2:29392/149‑1 (MQ=255)
                  ttCCAAGGGGGTGGGTTACGGGGTGCAGGCAAAGAT‑GGGCGGCTGGTCGCTGACCGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACctgc                                                                                                                                 >  3:132856/1‑149 (MQ=255)
                      aaGGGGGTGGGTTACGGGGTGCAGGCAAAGAT‑GGGCGGCTGGTCGCTGACCGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACctgctg                                                                                                                               >  2:767/1‑147 (MQ=255)
                       aGGGGGTGGGTTACGGGGTGCAGGCAAAGAT‑GGGCGGCTGGTCGCTGACCGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAg                                                                                                                            <  3:322280/149‑1 (MQ=255)
                        gggggTGGGTTACGGGGTGCAGGCAAAGAT‑GGGCGGCTGGTCGCTGACCGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAgg                                                                                                                           <  1:58973/149‑1 (MQ=255)
                        gggggTGGGTTACGGGGTGCAGGCAAAGAT‑GGGCGGCTGGTCGCTGACCGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAgg                                                                                                                           >  8:253567/1‑149 (MQ=255)
                        gggggTGGGTTACGGGGTGCAGGCAAAGAT‑GGGCGGCTGGTCGCTGACCGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAgg                                                                                                                           <  6:113819/149‑1 (MQ=255)
                          gggtgggtTACGGGGTGCAGGCAAAGAT‑GGGCGGCTGGTCGCTGACCGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGc                                                                                                                         >  3:280142/1‑149 (MQ=255)
                                           cAGGCAAAGAT‑GGGCGGCTGGTCGCTGACCGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCaa                                                                                                        <  4:132856/149‑1 (MQ=255)
                                             ggCAAAGAT‑GGGCGGCTGGTCGCTGACCGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAg                                                                                                       >  3:94051/1‑148 (MQ=255)
                                              gCAAAGAT‑GGGCGGCTGGTCGCTGACCGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGaa                                                                                                     >  6:86503/1‑149 (MQ=255)
                                                        ggcggcTGGTCGCTGACCGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGgc                                                                                            <  5:86503/149‑1 (MQ=255)
                                                            gcTGGTCGATGACCGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTTGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTg                                                                                        >  6:178356/1‑149 (MQ=255)
                                                              tGGTCGCTGACCGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGc                                                                                                          >  6:167273/1‑129 (MQ=255)
                                                              tGGTCGCTGACCGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGc                                                                                                          <  5:167273/129‑1 (MQ=255)
                                                                     tGACCGGC‑CGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGc                                                                               >  6:149882/1‑148 (MQ=255)
                                                                        ccGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTg                                                                                        >  6:299981/1‑137 (MQ=255)
                                                                        ccGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTg                                                                                        <  5:299981/137‑1 (MQ=255)
                                                                             ccGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCaa                                                                       <  5:149882/148‑1 (MQ=255)
                                                                               ggggTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCCGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAgg                                                                     <  4:94051/149‑1 (MQ=255)
                                                                                     ccAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGAt                                                                <  1:767/148‑1 (MQ=255)
                                                                                     ccAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGAt                                                                <  7:253567/148‑1 (MQ=255)
                                                                                        ggCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATGGc                                                             >  2:202807/1‑148 (MQ=255)
                                                                                         gCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATGGCaa                                                           >  8:31724/1‑149 (MQ=255)
                                                                                                   tCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATGGCAACGGTGCGGtt                                                 <  2:82495/149‑1 (MQ=255)
                                                                                                     aaCCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTTGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATGGCAACGGTGCGGTTgg                                               <  5:178356/149‑1 (MQ=255)
                                                                                                      aCCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTTGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATGGCAACGGTGCGGTTgg                                               >  4:266745/1‑148 (MQ=255)
                                                                                                       cccGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTAGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATGGCAACGGTGCGGTTgg                                               >  7:53914/1‑147 (MQ=255)
                                                                                                                  ccAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATgg                                                              <  4:46855/121‑1 (MQ=255)
                                                                                                                  ccAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATgg                                                              >  3:46855/1‑121 (MQ=255)
                                                                                                                   caacaaTGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATGGCAACGGTGCGGTTGGCAGCGGCGCGGAc                                  >  4:146333/1‑148 (MQ=255)
                                                                                                                   caacaaTGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATGGCAACGGTGCGGTTGGCAGCGGCGCGGAc                                  >  8:229986/1‑148 (MQ=255)
                                                                                                                   caacaaTGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGACCTATGGCAAGACCAAGGACGATGGCAAc                                                          >  8:93985/1‑124 (MQ=255)
                                                                                                                   caacaaTGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGACCTATGGCAAGACCAAGGACGATGGCAAc                                                          <  7:93985/124‑1 (MQ=255)
                                                                                                                      caaTGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATGGCAACGGTGCGGTTGGCAGCGGCGCGGACTAc                               >  5:135491/1‑148 (MQ=255)
                                                                                                                           ccGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCATATGGCAAGACCAAGGACGATGGCAACGGTGCGGTTGGCAGCGgcgct                                     >  6:103080/1‑136 (MQ=255)
                                                                                                                              gCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATGGCAACCGTGCGGTTGGCAGCGGCGCGGACTACGAGACCCgg                      >  7:119363/1‑149 (MQ=255)
                                                                                                                                 aaCCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATGGCAACGGTGCGGTTGGCAGCGGCGCGGACTACGAGACCCGGGgt                   <  4:280142/149‑1 (MQ=255)
                                                                                                                                      gTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAACTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAACGACGATGGCAACGGTGCGGTGGGCAGCGGCGCGGACTACGAGACCCGGGGTGTGgc              >  4:303188/1‑149 (MQ=255)
                                                                                                                                      gTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGAAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATGGCAACGGTGCGGTTGGCAGCGGCGCGGACTACGAGACCCGGGGTGTGgc              >  1:304429/1‑149 (MQ=255)
                                                                                                                                             gcAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATGGCAACGGTGCGGTTGGCAGCGGCGCGGACTACGAGACCCGGGGTGTGGCGCTGTTc       >  7:70382/1‑149 (MQ=255)
                                                                                                                                             gcAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATGGCAACGGTGCGGTTGGCAGCGGCGCGGACTACGAGACCCGGGGTGTGGCGCTGTTc       >  3:200559/1‑149 (MQ=255)
                                                                                                                                               aaTGTTGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATGGCAACGGTGCGGTTGGCAGCGGCGCGGACTACGAGACCCGGGGTGTGGCGCTGTTCCa     <  8:53914/149‑1 (MQ=255)
                                                                                                                                                 tGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATGGCAACGGTGCGGTTGGCAGCGGCGCGGACTACGAGACCCGGGGTGTGGCGCTGTTCCATg   <  7:31724/149‑1 (MQ=255)
                                                                                                                                                   tCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATGGCAACGGTGCGGTTGGCAGCGGCGCGGACTACGAGACCCGGGGTGTGGCGCTGTTCCATGa  <  2:304429/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CACGGTAGAAACGATCACTTCCAAGGGGGTGGGTTACGGGGTGCAGGCAAAGAT‑GGGCGGCTGGTCGCTGACCGGCTCGGGGTTCCAGGCCAAAGGCATCAACCCGTTCTTCACCAACAATGCCGGCGAACCGGTTTTGCGCAATGTCGACAGTGATGGCTACCTGCTGCAGGGCTCGTACAAGTTCGGCAAGAACCGCGTGGCGCTGTCCTATGGCAAGACCAAGGACGATGGCAACGGTGCGGTTGGCAGCGGCGCGGACTACGAGACCCGGGGTGTGGCGCTGTTCCATGA  >  NC_002947/3049614‑3049907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑