Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,053,249 C→T 100% R221H (CGT→CAT)  agmR ← glycerol metabolism activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,053,2490CT96.8% 133.2 / ‑4.1 45R221H (CGT→CAT) agmRglycerol metabolism activator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (18/25);  minor base A (2/0);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGAACTGGGTCACACGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCT‑CAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACGGTGGTTTCGGCGATGTCCAGCGTGTAGGCGATCTGCTTGTT  >  NC_002947/3053108‑3053394
                                                                                                                                              |                                                                                                                                                 
gcgAACTGGGTCACACGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTAGCGGGTTGATCCGCGAAGCGGCCGGAACACCT‑CAATGgcg                                                                                                                                               >  3:114905/1‑146 (MQ=255)
   aaCTGGGTCACACGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATa                                                                                                                                         <  2:259877/149‑1 (MQ=255)
    acggggTCACCCGCTGATGCACAATCCCGTACCACCGATGAAACTCACCCCGGCGCTGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATaa                                                                                                                                        <  2:263950/146‑1 (MQ=255)
      tGGGTCACCGGGTGATGCACAAGCGCGTAAGCCCGATGAAACTCAACCCGGCGATGAGCCTGTTGCTGGCCTGTGGCTTGGATTAGCGGTTGGCCGCTTCGCGGGTTGAGCCGCTATGGGGCCGGAACAGCT‑CAATGGCGCAGATAATc                                                                                                                                      <  4:66701/149‑1 (MQ=255)
              acGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGACCAGTGGCTTGGCATAGCGGTTGGCCCCATAGCGGGTTTATACGCCAAGCGGCCGGAAAAGCG‑AAAATGCGCAGATAATAAACaaaa                                                                                                                               >  2:136392/1‑148 (MQ=255)
                    aTGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCCCATAGGGGGTTGATAAGCGCAACGGCCGGCACAGATCCAATGGC‑CCGATAATCCCCaaaaacctaa                                                                                                                         >  3:130162/1‑142 (MQ=255)
                    aTGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCCCAAGGCGGGTTTATAAGCGAAGCGGGCCGAAAAGCT‑CAAATGGGCACAAAATAACCaaaaaccaaa                                                                                                                         >  3:125152/1‑142 (MQ=255)
                       cacaAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCAGGTTGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGc                                                                                                                     >  7:190140/1‑149 (MQ=255)
                                        gATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATAGCCAGCACTGAGGATGGCt                                                                                                    >  5:63244/1‑149 (MQ=255)
                                        gATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCG‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCt                                                                                                    >  7:232396/1‑149 (MQ=255)
                                             aaCTCAAGACGCCTATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCTAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTa                                                                                                <  5:280045/148‑1 (MQ=255)
                                                  aaGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGGTGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATGGCCAGCACTGAGGATGGCTTGTACCCGGt                                                                                          >  7:90930/1‑149 (MQ=255)
                                                     aCGGCGATTAGCCATGTGCTGGCCTGTGGTTTGGAATAGGGGTTGGCCGCTTCGCTGGTTTATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTtgt                                                                                       <  8:190140/149‑1 (MQ=255)
                                                     aCGCCGATGAGCCATATGCTTGCCAGTGGCGTGGCATAGCGGTTGTCCGCTTCGCGGTTTGATCCGCGAAGCGGCCGGAACATCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTtgt                                                                                       <  1:55972/149‑1 (MQ=255)
                                                          gATGACCCATCTGCTGGCCAGGGGCTTGGCATCGCGGTTGGCCGCTTCGCGGGTTGACCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGcaca                                                                                  <  2:223960/149‑1 (MQ=255)
                                                                          gCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCaaaa                                                                  >  6:141295/1‑149 (MQ=255)
                                                                             aGTGGCTTGGCATAGCGGTTGGCCGCTTTGCGGGTTGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCg                                                               >  5:58975/1‑149 (MQ=255)
                                                                                       cATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTg                                                     <  5:141295/149‑1 (MQ=255)
                                                                                       cATAGCGGTTGGCCGATTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTg                                                     <  4:114905/149‑1 (MQ=255)
                                                                                                tGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGAc                                            >  6:150114/1‑149 (MQ=255)
                                                                                                   ccGCTTGGCGGGTTGATCCGCTAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACggt                                         <  1:136392/149‑1 (MQ=255)
                                                                                                   ccGCTTCGCGGGTGGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACggt                                         <  8:71118/149‑1 (MQ=255)
                                                                                                     gCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTAACCGGTTGTGCAGATTGAGTTTGCGCAAAATCGCCGGAACGTGGGCCTTGACggtgg                                       >  5:129381/1‑149 (MQ=255)
                                                                                                       ttCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTgc                                                                        <  2:218737/114‑1 (MQ=255)
                                                                                                          gggggTTGATCGGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACGGTGGTTTCg                                  <  5:315129/147‑1 (MQ=255)
                                                                                                           cgGGTTGATCGGCTAAGGGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCTGAAACGTGGGCCTTGACGGTGGTTTCgg                                 <  1:186469/149‑1 (MQ=255)
                                                                                                            gggTTGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACGGTGGTTTCGGc                                >  4:172582/1‑149 (MQ=255)
                                                                                                             gggtGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACGGTGGTTTCGGCg                               <  4:130162/146‑1 (MQ=255)
                                                                                                             ggTTGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACGGTGGTTTCGGCg                               <  4:125152/149‑1 (MQ=255)
                                                                                                              gTTGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTgg                                                    >  4:312867/1‑127 (MQ=255)
                                                                                                              gTTGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTgg                                                    <  3:312867/127‑1 (MQ=255)
                                                                                                              gTTGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACGGTGGTTTCGGCGa                              >  2:83906/1‑149 (MQ=255)
                                                                                                               ttGATCCGCGAAGCGTCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACGGTGGTTTCGGCGAt                             <  6:107711/149‑1 (MQ=255)
                                                                                                               ttGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACGGTGGTTTCGGCGAt                             <  8:232396/149‑1 (MQ=255)
                                                                                                                ttttCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACGGTGGTTTCGGCGa                              <  1:282039/144‑1 (MQ=255)
                                                                                                                tGATCCGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACGGTGGTTTCGGCGAt                             <  6:63244/148‑1 (MQ=255)
                                                                                                                    ccGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACggtg                                        <  5:199638/133‑1 (MQ=255)
                                                                                                                    ccGCGAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACggtg                                        >  6:199638/1‑133 (MQ=255)
                                                                                                                      gcgAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACGGTGGTTTCGGCGATGTCCAGc                      >  6:216489/1‑149 (MQ=255)
                                                                                                                        gAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGa                                             <  6:209466/124‑1 (MQ=255)
                                                                                                                        gAAGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGa                                             >  5:209466/1‑124 (MQ=255)
                                                                                                                         aaGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTAcc                                                                                              <  6:157797/74‑1 (MQ=255)
                                                                                                                         aaGCGGCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTAcc                                                                                              >  5:157797/1‑74 (MQ=255)
                                                                                                                             ggCCGGAACAGCT‑CAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACGGTGGTTTCGGCGATGTCCAGCGTGTAgg               <  1:83906/149‑1 (MQ=255)
                                                                                                                                           cAATGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACGGTGGTTTCGGCGATGTCCAGCGTGTAGGCGATCTGCTTGtt  >  8:97372/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
GCGAACTGGGTCACACGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCT‑CAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACGGTGGTTTCGGCGATGTCCAGCGTGTAGGCGATCTGCTTGTT  >  NC_002947/3053108‑3053394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑