Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,087,768 G→C 11.7% R259R (CGC→CGG) ‡ metR‑II ← homocysteine‑binding DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,087,7680GC11.7% 144.3 / 8.2 50R259R (CGC→CGG) ‡metR‑IIhomocysteine‑binding DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (22/22);  new base C (3/3);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACCGGGCCTGACGCCACGCTAACACCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAGTACGTACCTGTGCGGGCTCGACGTCGGCGGG  >  NC_002947/3087621‑3087914
                                                                                                                                                   |                                                                                                                                                   
aaCCGGGCCTGACGCCACGCTAACACCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGgc                                                                                                                                                    <  3:29615/149‑1 (MQ=255)
  ccggtacTGAAGCCACGCTAACACATGCCAGAGTGGATAACGATATGTCCTTGGCAGTGAGAAGAAAATAACGCATGTAAGTCACATCAAGAATGTCACTGCGAAGTGCAGCATAGAGGTTTGCCTGCAGGCAGTGATCGCCAAGgagc                                                                                                                                                  >  6:154611/7‑149 (MQ=255)
    gggCCTGACGCCACGCTAACACCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑tt                                                                                                                                               <  3:54017/149‑1 (MQ=255)
        cTGACGCCACGCTAACACCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCg                                                                                                                                           <  1:272853/149‑1 (MQ=255)
        cTGACGCCACGCTAACACCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCg                                                                                                                                           <  4:36073/149‑1 (MQ=255)
          gACGCCACGCTAACACCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGt                                                                                                                                         >  3:275547/1‑149 (MQ=255)
            cGCCACGCTAACACCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTa                                                                                                                                       <  2:19177/149‑1 (MQ=255)
                                      tgAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTg                                                                                                              >  3:107765/1‑148 (MQ=255)
                                       gAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCa                                                                                                            >  3:244498/1‑149 (MQ=255)
                                          cGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAAc                                                                                                                        >  1:158036/1‑134 (MQ=255)
                                          cGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAAc                                                                                                                        <  2:158036/134‑1 (MQ=255)
                                             gaTGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGcc                                                                                                       >  7:96645/1‑148 (MQ=255)
                                                 tCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGCGCC‑TTGGCga                                                                                                                                          >  5:1175/1‑108 (MQ=255)
                                                 tCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGCGCC‑TTGGCga                                                                                                                                          >  5:5829/1‑108 (MQ=255)
                                                 tCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGCGCC‑TTGGCga                                                                                                                                          <  6:1175/109‑2 (MQ=255)
                                                 tCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGCGCC‑TTGGCga                                                                                                                                          <  4:177359/109‑2 (MQ=255)
                                                 tCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGCGCC‑TTGGCga                                                                                                                                          >  3:177359/1‑108 (MQ=255)
                                                 tCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGCGCC‑TTGGCga                                                                                                                                          <  6:5829/109‑2 (MQ=255)
                                                   cTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTgg                                                                                                >  7:184889/1‑149 (MQ=255)
                                                           tGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCa                                                                                                     <  4:25279/136‑1 (MQ=255)
                                                           tGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCa                                                                                                     <  4:20979/136‑1 (MQ=255)
                                                           tGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCa                                                                                                     >  3:25279/1‑136 (MQ=255)
                                                           tGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCa                                                                                                     >  3:20979/1‑136 (MQ=255)
                                                                     aTCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGAcgccgc                                                                              >  7:124254/1‑149 (MQ=255)
                                                                       cACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGaa                                                                                                                         <  4:274248/104‑1 (MQ=255)
                                                                       cACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGaa                                                                                                                         >  3:274248/1‑104 (MQ=255)
                                                                        aCGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGcc                                                                           >  2:181819/1‑149 (MQ=255)
                                                                             tGTACGGCACCTCCATCATTTGACTGCGCAGTTCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATATCCGCGCTAACTTTATTCGTGCACGGCCCAGTTGTGCAGGCAGCAGACGCCGCGCCCGGaa                                                                      <  4:201914/149‑1 (MQ=255)
                                                                              gTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAg                                                                     >  7:294375/1‑149 (MQ=255)
                                                                                       cTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGtt                                                            >  1:139181/1‑149 (MQ=255)
                                                                                       cTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGtt                                                            <  4:275547/149‑1 (MQ=255)
                                                                                       cTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGtt                                                            <  8:166137/149‑1 (MQ=255)
                                                                                       cTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGtt                                                            <  1:181819/149‑1 (MQ=255)
                                                                                          cagcagGTCACTGCGCAGGGCAGCTTAGCGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGGGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGca                                                         <  7:97948/149‑1 (MQ=255)
                                                                                                gTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGcatcatca                                                   >  8:233116/1‑149 (MQ=255)
                                                                                                 tgaCTGCGAAGTTCAGCATAGTGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATTGCAGCGCGAACTGTATTCGTGGACGGCCCAGTTGGTCAGGCAGCAGACGGCGCGCCCGGAAGCAACCTGtt                                                            <  6:98469/137‑1 (MQ=255)
                                                                                                 tCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAAACAGtt                                                            >  5:98469/1‑139 (MQ=255)
                                                                                                   aCTGCGCAGTGCGGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGcatcatcat                                                  >  1:86649/1‑148 (MQ=255)
                                                                                                           tgcgagcaTAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAg                                         <  6:174565/144‑1 (MQ=255)
                                                                                                              cagcaTAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTc                                      <  8:96645/148‑1 (MQ=255)
                                                                                                                gcaTAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGggcagg                                                                                           <  6:187127/93‑1 (MQ=255)
                                                                                                                gcaTAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGggcagg                                                                                           >  5:187127/1‑93 (MQ=255)
                                                                                                                gcaTAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGa                                   <  8:124254/149‑1 (MQ=255)
                                                                                                                  aTAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAg                                 <  4:107765/149‑1 (MQ=255)
                                                                                                                        gTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCAGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAgtacgta                           >  2:315825/1‑149 (MQ=255)
                                                                                                                          ttGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAGTACGTAcc                         >  8:209993/1‑149 (MQ=255)
                                                                                                                                gCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAAcc                                                                >  3:182323/1‑104 (MQ=255)
                                                                                                                                gCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAAcc                                                                <  4:182323/104‑1 (MQ=255)
                                                                                                                                           tCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAGTACGTACCTGTGCGGGCTCGACGTc        >  3:81983/1‑149 (MQ=255)
                                                                                                                                                aaGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAGTACGTACCTGTGCGGGCTCGACGTcggcgg   <  7:68838/149‑1 (MQ=255)
                                                                                                                                                aaGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAGTACGTACCTGTGCGGGCTCGACGTcggcgg   >  8:68838/1‑149 (MQ=255)
                                                                                                                                                 aGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAGTACGTACCTGTGCGGGCTCGACGTCGGCggg  <  8:294375/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AACCGGGCCTGACGCCACGCTAACACCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGC‑TTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAGTACGTACCTGTGCGGGCTCGACGTCGGCGGG  >  NC_002947/3087621‑3087914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑