Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,097,126 G→A 8.7% A44T (GCC→ACC)  PP_2705 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,097,1260GA 8.7% 157.8 / 4.5 57A44T (GCC→ACC) PP_2705hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/24);  new base A (3/2);  total (31/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.95e-01

GAAGCGCGTCGTGACAGGCTTCATCCCGTTATCGTCGCTCTGGCGATTCACACCGACGGACCAGGCTCGGTCCAAAGAGGGTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCAGAACCAGTGCCAGCTGTTTTATGCCGAAGTGCGCAAGAGGG  >  NC_002947/3096987‑3097273
                                                                                                                                             |                                                                                                                                                   
gAAGCGCGTCGTGACAGGCTTCATCCCGTTATCGTCGCTCTGGCGATTCACACCGACGGACCAGGCTCGGTCCAAAGAGGGTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGCTTCAACctgctgca                                                                                                                                            <  8:137893/149‑3 (MQ=255)
        tcgtGACAGGCGTCATCCCGGTTTCGTCGCTCGTGCGATTCAAACCGACGGACCAGGCTCGGGCCCAACTCGGTGCTGCAATGGCGTCTATCGAGTACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGta                                                                                                                                     <  1:251659/149‑1 (MQ=255)
               aGGCTTCATCCCGTTATCGTCGCTCTGGCGATTCACACCGACGGACCAGGCTCGGTCCAAAGAGGGTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGt                                                                                                                             >  1:245014/1‑149 (MQ=255)
                 gCTTCATCCCGTTATCGTCGCTCTGGCGATTCACACCGACGGACCAGGCTCGGTCCAAAGAGGGTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAGGGGGCTGCCAGCAGGCTTCAACctgctgcagc                                                                                                                                          <  1:250601/135‑5 (MQ=255)
                 gCTTCATCCCGTTATCGTCGCTCTGGCGATTCACACCGACGGACCAGGCTCGGTCCAAAGAGGGTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAGGGGGCTGCCAGCAGGCTTCAACctgctgcagc                                                                                                                                          >  2:250601/1‑131 (MQ=255)
                       tCCCGTTATCGTCGCTCTGGCGATTCACACCGACGGACCAGGCTCGGTCCAAAGAGGGTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCgg                                                                                                                     >  6:108602/1‑149 (MQ=255)
                        cccGTTATCGTCGCTCTGGCGATTCACACCGACGGACCAGGCTCGGTCCAAAGAGGGTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGCCGTATACCTCGTCACAGCGGc                                                                                                                    >  8:209392/1‑149 (MQ=255)
                                 gtcgCTCTGGCGATTCACACCGACGGACCAGGCTCGGTCCAAAGAGGGTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGaa                                                                                                            <  5:108602/148‑1 (MQ=255)
                                    gCTCTGGCGATTCACACCGACGGACCAGGCTCGGTCCAAAGAGGGTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGcc                                                                                                        >  8:250807/1‑149 (MQ=255)
                                          gCGATTCACACCGACGGACCAGGCTCGGTCCAAAGAGGGTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTcgcg                                                                                                   <  7:250807/148‑1 (MQ=255)
                                                   acCGACGGACCAGGCTCGGTCCAAAGAGGGTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAg                                                                                                           >  4:143811/1‑131 (MQ=255)
                                                   acCGACGGACCAGGCTCGGTCCAAAGAGGGTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAg                                                                                                           <  3:143811/131‑1 (MQ=255)
                                                         ggACCAGGCTCGGTCCAAAGATGGTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGaa                                                                                                            <  6:85693/124‑1 (MQ=255)
                                                                    ggTCCAAAGAGGGTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGa                                                                         >  6:142548/1‑148 (MQ=255)
                                                                     gTCCAAAGAGGGTGCTGCAATGGCGACAATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGAGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGaaa                                                                       >  1:183456/1‑149 (MQ=255)
                                                                         aaaGAGGGTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGAc                                                                                          >  6:153105/1‑126 (MQ=255)
                                                                         aaaGAGGGTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGAc                                                                                          <  5:153105/126‑1 (MQ=255)
                                                                              gggTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCAtt                                                                           <  6:220875/136‑1 (MQ=255)
                                                                              gggTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCAtt                                                                           >  5:220875/1‑136 (MQ=255)
                                                                               ggTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAACCCGCCAACcg                                                             >  8:306520/1‑149 (MQ=255)
                                                                               ggTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGACACCcg                                                             >  8:20519/1‑149 (MQ=255)
                                                                                 tgctgcAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACcgc                                                            >  7:308557/1‑148 (MQ=255)
                                                                                    tgcAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGa                                                          >  3:229376/1‑147 (MQ=255)
                                                                                    tgcAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGa                                                          <  4:229376/147‑1 (MQ=255)
                                                                                     gcAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATAcc                                                                                                                                 <  7:44105/75‑1 (MQ=255)
                                                                                     gcAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATAcc                                                                                                                                 >  8:44105/1‑75 (MQ=255)
                                                                                     gcAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGAttt                                                       >  7:98738/1‑149 (MQ=255)
                                                                                        aTGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCAc                                                    <  5:142548/149‑1 (MQ=255)
                                                                                             gACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGt                                               >  2:33455/1‑149 (MQ=255)
                                                                                                  tCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCg                                                                                                                                       >  2:286407/1‑56 (MQ=255)
                                                                                                  tCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCg                                                                                                                                       <  1:286407/56‑1 (MQ=255)
                                                                                                  tCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGttt                                             >  3:110897/1‑146 (MQ=255)
                                                                                                     a‑cacCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCa                                           >  4:183717/1‑145 (MQ=255)
                                                                                                     a‑cacCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCa                                           <  3:183717/145‑1 (MQ=255)
                                                                                                     a‑cacCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCAGAAc                                       <  7:209392/149‑1 (MQ=255)
                                                                                                          cGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCACCCGCGAt                                                         >  8:254181/1‑127 (MQ=255)
                                                                                                            agaACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCg                                                                                        <  1:257192/94‑1 (MQ=255)
                                                                                                            agaACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCg                                                                                        >  2:257192/1‑94 (MQ=255)
                                                                                                                  ttGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCAGAACCAGTGCCAGCTg                           >  2:128349/1‑149 (MQ=255)
                                                                                                                      aaaGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGa                                                          <  5:306038/114‑1 (MQ=255)
                                                                                                                      aaaGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGa                                                          >  6:306038/1‑114 (MQ=255)
                                                                                                                      aaaGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCa                                           >  3:111790/1‑129 (MQ=255)
                                                                                                                      aaaGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCa                                           <  4:111790/129‑1 (MQ=255)
                                                                                                                      aaaGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCAGAACCAGt                                   <  5:86536/137‑1 (MQ=255)
                                                                                                                      aaaGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCAGAACCAGt                                   >  6:86536/1‑137 (MQ=255)
                                                                                                                        aGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACGGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCAGAACCAGTGCCAGCTGTTTTAt                     <  5:143385/149‑1 (MQ=255)
                                                                                                                        aGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCAGAACCAGTGCCAGCTGTTTTAt                     <  8:308557/149‑1 (MQ=255)
                                                                                                                             ctgccagcagGCTTCAACCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCAGAACCAGTGCCCGCTGTTTTATGcc                  >  4:10774/5‑148 (MQ=255)
                                                                                                                                ccagcagGCTTCAACCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTTCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCAGAACCAGTGCCAGCTGTTTTATGCCGAAg              >  3:223001/2‑149 (MQ=255)
                                                                                                                                 cagcagGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCAGAACCAGTGCCAGCTGTTTTATGCCGAAGt             <  8:98738/149‑1 (MQ=255)
                                                                                                                                   gcagGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCAGAACCAGTGTCAGCTGttt                        >  6:274344/1‑136 (MQ=255)
                                                                                                                                   gcagGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCAGAACCAGTGTCAGCTGttt                        <  5:274344/136‑1 (MQ=255)
                                                                                                                                      ggTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAACCCGCCAACCGCGATTTCACCAGGTTTCAGAACCAGTGCCAGCTGTTTTa                      >  6:331121/1‑135 (MQ=255)
                                                                                                                                      ggTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCAGAACCAGTGCCAGCTGTTTTa                      <  5:331121/135‑1 (MQ=255)
                                                                                                                                           aaGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCAGAACCAGTGCCAGCTGTTTTATGCCGAAGTGCGCAagag    <  1:33455/148‑1 (MQ=255)
                                                                                                                                           aaGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCAGAACCAGTGCCAGCTGTTTTATGCCGAAGTGCGCAAGAgg   >  5:59074/1‑149 (MQ=255)
                                                                                                                                            aGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCAGAACCAGTGCCAGCTGTTTTATGCCGAAGTGCGCAAGAggg  <  1:128349/149‑1 (MQ=255)
                                                                                                                                             gCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCGCCAGGTTTCAGAACCAGTGCCAGCTGTTTTATGCCGAAGTGCGCAAGAggg  >  3:227376/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
GAAGCGCGTCGTGACAGGCTTCATCCCGTTATCGTCGCTCTGGCGATTCACACCGACGGACCAGGCTCGGTCCAAAGAGGGTGCTGCAATGGCGACTATCGA‑CACCGAGAACCTTGCAAAGGGGCTG‑CAGCAGGTTGAAGCCTGCTGGCAGGCGTATACCTCGTCACAGCGGCTTGTCGAAGGCCTCGCGGGCAGCGACCGGAGCAGTGCCATTGAAACCGCCAACCGCGATTTCACCAGGTTTCAGAACCAGTGCCAGCTGTTTTATGCCGAAGTGCGCAAGAGGG  >  NC_002947/3096987‑3097273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑