Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	344,032	A→G	10.5%	intergenic (+121/+68)	PP_5440 → / ← PP_t72	hypothetical protein/tRNA‑Phe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	344,032	0	A	G	10.5%	171.8 / 8.3	66	intergenic (+121/+68)	PP_5440/PP_t72	hypothetical protein/tRNA‑Phe
Reads supporting (aligned to +/- strand):  ref base A (32/27);  new base G (3/4);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGTTCCATGTCGGACGAGTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGA  >  NC_002947/343889‑344180                                                                                                                                                 |                                                                                                                                                      ttCGTTCCATGTCGGACGAGTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAAcc                                                                                                                                                  >  2:112615/1‑149 (MQ=255) ttCGTTCCATGTCGGACGAGTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAAcc                                                                                                                                                  >  8:111107/1‑149 (MQ=255)   cGTTCCATGTCGGACGAGTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAAccc                                                                                                                                                 <  4:23749/148‑1 (MQ=255)   cGTTCCATGTCGGACGAGTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAAccc                                                                                                                                                 >  5:6945/1‑148 (MQ=255)   cGTTCCATGTCGGACGAGTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAAcc                                                                                                                                                  <  3:204276/147‑1 (MQ=255)     ttCCATGTCGGACGAGTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCa                                                                                                                                              >  7:77024/1‑149 (MQ=255)      tCCATGTCGGACGAGTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAGAAACCCGcc                                                                                                                                              >  2:96606/1‑147 (MQ=255)                  gAGTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAGAAACCCGC‑CTAGTGCGGGtttt                                                                                                                                >  4:144534/1‑148 (MQ=255)                  gAGTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTc                                                                                                                                >  5:329467/1‑149 (MQ=255)                    gTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCtt                                                                                                                              <  7:111107/149‑1 (MQ=255)                       aGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAGAAACCCGC‑CTAGTGCGGGTTTTttgtt                                                                                                                           <  1:96606/149‑1 (MQ=39)                       aGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAGAAACCCGC‑CTAGTGCGGGTTTTttgtt                                                                                                                           <  3:144534/149‑1 (MQ=39)                         gTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTtgttgt                                                                                                                         >  4:940/1‑149 (MQ=255)                          tttGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCgg                                                                                                                                     <  7:135738/136‑1 (MQ=255)                          tttGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCgg                                                                                                                                     >  8:135738/1‑136 (MQ=255)                             ggCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAGAAACCCGC‑CTAGTGCGGGTTTTTTGTTGTctg                                                                                                                      <  7:1410/148‑3 (MQ=38)                               cccGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCGTc                                                                                                                   >  8:247903/1‑149 (MQ=255)                                ccGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCg                                                                                                                  >  4:297863/1‑149 (MQ=255)                                      tCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCGAATGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTa                                                                                                              >  1:247350/1‑148 (MQ=255)                                          cTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACttt                                                                                                         <  3:940/148‑1 (MQ=255)                                          cTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACtt                                                                                                          <  3:57126/147‑1 (MQ=255)                                           tCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCttgtt                                                                                                                           >  2:169332/1‑129 (MQ=255)                                           tCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCttgtt                                                                                                                           <  1:169332/129‑1 (MQ=255)                                           tCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGc                                                                                                       <  6:6945/149‑1 (MQ=255)                                                        tttCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGc                                                                                          >  6:255996/1‑149 (MQ=255)                                                            tcGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCgg                                                                                                                 >  7:224247/1‑122 (MQ=255)                                                            tcGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCgg                                                                                                                 <  8:224247/122‑1 (MQ=255)                                                            tcGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTg                                                                                       >  6:298395/1‑148 (MQ=255)                                                              gATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGATGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGaa                                                                                     <  8:91807/148‑1 (MQ=255)                                                              gATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAg                                                                                    <  5:255996/149‑1 (MQ=255)                                                              gATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAg                                                                                    >  7:271006/1‑149 (MQ=255)                                                               aTTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGc                                                                                                                      <  5:121262/114‑1 (MQ=255)                                                               aTTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGc                                                                                                                      >  6:121262/1‑114 (MQ=255)                                                                     tGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGc                                                                             <  1:112615/149‑1 (MQ=255)                                                                             cTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGAc                                                                     <  2:294469/149‑1 (MQ=255)                                                                              ttCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTtgttgt                                                                                                                         <  8:171567/96‑1 (MQ=255)                                                                              ttCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTtgttgt                                                                                                                         >  7:171567/1‑96 (MQ=255)                                                                                cAGGTTGCG‑TTGTGAGGTCTAGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACgg                                                                   <  8:271006/148‑1 (MQ=255)                                                                                       cG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCGTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGaa                                                            <  7:247903/148‑1 (MQ=255)                                                                                        g‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCa                                                                          >  5:101294/1‑133 (MQ=255)                                                                                        g‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCa                                                                          <  6:101294/133‑1 (MQ=255)                                                                                               aGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACttt                                                                                                         >  4:142022/1‑96 (MQ=255)                                                                                                ggTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGaca                                                   >  7:35365/1‑149 (MQ=255)                                                                                                     cGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAg                                               >  1:48530/1‑148 (MQ=255)                                                                                                                  gccgccTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCctc                                 <  6:329467/149‑1 (MQ=255)                                                                                                                      ccTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGct                             <  5:298395/149‑1 (MQ=255)                                                                                                                        tGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCTACACGAGGATTTTCAATCCTCTGctc                            >  2:188974/1‑148 (MQ=255)                                                                                                                        tGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGctc                            >  4:133307/1‑148 (MQ=255)                                                                                                                         gAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTa                          >  1:54260/1‑149 (MQ=255)                                                                                                                            gTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGAGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTAcc                        >  4:288333/1‑148 (MQ=255)                                                                                                                                 aaaTCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGa                  <  2:54529/149‑1 (MQ=255)                                                                                                                                    tCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCt               <  2:54260/149‑1 (MQ=255)                                                                                                                                         gACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCgg                                                                                                                 >  6:159336/1‑46 (MQ=255)                                                                                                                                         gACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCgg                                                                                                                 <  5:159336/46‑1 (MQ=255)                                                                                                                                          acaacaAGAAACCCGC‑CTAGTGCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTgg         >  4:194323/1‑149 (MQ=255)                                                                                                                                          acaacaAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACgg                                                                   <  7:17435/91‑1 (MQ=255)                                                                                                                                          acaacaAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACgg                                                                   >  8:17435/1‑91 (MQ=255)                                                                                                                                          acaacaAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGa                  >  3:313646/1‑140 (MQ=255)                                                                                                                                          acaacaAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGa                  <  4:313646/140‑1 (MQ=255)                                                                                                                                          acaacaAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTAGCAACCACCAGAAGCTTGAAGCTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTATCAATCCTCTGCTCTACCGACTGAGCTATcagt         >  1:48599/1‑146 (MQ=255)                                                                                                                                            aacaaGAAACCCGC‑CTAGTGCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGc       <  3:194323/149‑1 (MQ=255)                                                                                                                                            aacaaAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGc       >  6:39684/1‑149 (MQ=255)                                                                                                                                            aacaaAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGc       <  8:77024/149‑1 (MQ=255)                                                                                                                                              caaAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAAccgcc                                                       <  7:87601/99‑1 (MQ=255)                                                                                                                                              caaAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAAccgcc                                                       >  8:87601/1‑99 (MQ=255)                                                                                                                                              caaAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTATCTTCGGGTAACTTTGAAACAACCAGAAGCTTGAAGGTGGTGACCAGAGACGGAGTCGAAACGCCGAAACGATGATTTTCAATAATCTGATCTAAAGACTGAGCTATCTGGGcg      >  5:150827/1‑148 (MQ=255)                                                                                                                                               aaaaaaCCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGAtttt                                         >  5:73093/1‑112 (MQ=255)                                                                                                                                               aaaaaaCCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGAtttt                                         <  6:73093/112‑1 (MQ=255)                                                                                                                                                 aaaaCCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGcgacga  <  2:247350/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                      TTCGTTCCATGTCGGACGAGTAAGGTTTGGCCCGCAGTCTGCTCATGGCTGCGGGTTTCTCGATTGCATGGCTAGCCTTCAGGTTGCG‑TTGTGAGGTCTCGTGTTCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGA  >  NC_002947/343889‑344180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑