Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,123,185 G→C 7.3% G207A (GGG→GCG) ‡ PP_2740 → MerR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,123,1850GC 7.3% 173.3 / 4.2 55G207A (GGG→GCG) ‡PP_2740MerR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (26/25);  new base C (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.82e-01

CTGCTGCGCTTGCAGATGAACCAGGTCGATGCGCCGC‑GGGTGCTGCTGGCGGGTACCGAGGGTCAAGCCGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGCGCTCGCTGCAGATGGAAATTGATTGTCCGCTGGCCTTGAC  >  NC_002947/3123046‑3123329
                                                                                                                                            |                                                                                                                                                
ctgctgcGCTTGCAGATGAACCAGGTCGATGCGCCGC‑GGGTGCTGCTGGCGGGTACCGAGGGTCAAGCCGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGc                                                                                                                                          <  2:281002/148‑1 (MQ=255)
   ctgcGCTTGCAGATGAACCAGGTCGATGCGCCGC‑GGGTGCTGCTGGCGGGTACCGAGGGTCAAGCCGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCCCGCAGCCgagc                                                                                                                                        <  7:156526/147‑4 (MQ=255)
    tgcGCTTGCAGATGAACCAGGTCGATGCGCCGC‑GGGTGCTGCTGGCGGGTACCGAGGGTCAAGCCGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTgg                                                                                                                                       >  5:263435/1‑147 (MQ=255)
           gCAGATGAACCAGGTCGATGCGCCGCGGGGTGCTGCTGGCGGGTACCGAGGGTCAAGCCGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTc                                                                                                                               >  1:19557/1‑149 (MQ=255)
              gATGAACCAGGTCGATGCGCCGC‑GGGTGCTGCTGGCGGGTACCGAGGGTCAAGCCGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCgc                                                                                                                           >  4:46651/1‑149 (MQ=255)
                ggatccAGGTCGATGCGCCGC‑GTGTGCTGCTGGCGGGTTCCGAGGGTCAAGCCGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGATCGACAACGCATCGTCGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCgctg                                                                                                                         <  2:60820/145‑1 (MQ=255)
                   aCCAGGTCGATGCGCCGC‑GGGTGCTGCTGACGGGTACCGAGGGTCAAGCCGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGctgctg                                                                                                                      >  1:24762/1‑149 (MQ=255)
                    ccAGGTCGATGCGCCGC‑GGGTGCTGCTGGCGGGTACCGAGGGTCAAGCCGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCgctgcaca                                                                                                                     >  1:78287/1‑146 (MQ=255)
                       ggTCGATGCGCCGC‑GGGTGCTGCTGGCGGGTACCGAGGGTCAAGCCGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCtgtgtg                                                                                                                  <  8:62279/149‑1 (MQ=255)
                            aTGCGCCGC‑GGGTGCTGCTGGCGGGTACCGAGGGTCAAGCCGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCg                                                                                                             <  2:24762/149‑1 (MQ=255)
                               cgccgc‑GGGTGCTGCTGGCGGGTACCGAGGGTCAAGCCGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGt                                                                                                           <  2:19557/148‑1 (MQ=255)
                                 ccgc‑GGGTGCTGCTGGCGGGTACCGAGGGTCAAGCCGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCa                                                                                                        <  3:312546/149‑1 (MQ=255)
                                               tgGCGGGTACCGAGGGTCAAGCCGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTg                                                                                           <  8:64372/149‑1 (MQ=255)
                                                       aCCGAGGGTCAAGCCGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATcgc                                                                                   >  5:58725/1‑149 (MQ=255)
                                                        ccGATGGTCAAGCCGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCGGCCTGCGTCACTGGTGATcgcg                                                                                  <  6:263435/149‑1 (MQ=255)
                                                          gAGGGTCAAGCCGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCt                                                                                >  7:138227/1‑149 (MQ=255)
                                                                  aGCCGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCGGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCAc                                                                        <  6:110816/149‑1 (MQ=255)
                                                                    ccGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCgg                                                                                                >  5:85136/1‑123 (MQ=255)
                                                                    ccGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCgg                                                                                                <  6:85136/123‑1 (MQ=255)
                                                                      gAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGt                                                                    >  4:315716/1‑149 (MQ=255)
                                                                      gAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGt                                                                    >  4:38837/1‑149 (MQ=255)
                                                                      gAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGt                                                                    >  1:31807/1‑149 (MQ=255)
                                                                             aGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCTCTGGTGATCGCGCTGCAGCCACCGGTGAGCCCt                                                             >  5:158423/1‑149 (MQ=255)
                                                                             aGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCt                                                             >  2:308660/1‑149 (MQ=255)
                                                                                  ttgtGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCtt                                                        <  6:58725/149‑1 (MQ=255)
                                                                                    gtgCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTgg                                                      >  8:120533/1‑149 (MQ=255)
                                                                                        gcCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCaa                                                  <  2:31807/149‑1 (MQ=255)
                                                                                              ctgctgTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCt                                            <  8:138227/149‑1 (MQ=255)
                                                                                                 ctgTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTg                                                       <  7:91418/135‑1 (MQ=255)
                                                                                                 ctgTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTg                                                       >  8:91418/1‑135 (MQ=255)
                                                                                                  tgTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCa                                                                             >  4:257617/1‑112 (MQ=255)
                                                                                                  tgTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCa                                                                             <  3:257617/112‑1 (MQ=255)
                                                                                                   gTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGa                                                                  <  4:201430/122‑1 (MQ=255)
                                                                                                   gTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGa                                                                  >  3:201430/1‑122 (MQ=255)
                                                                                                   gTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGcgct                                       >  3:1316/1‑149 (MQ=255)
                                                                                                       agcagcGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGcgctcgct                                   >  7:301943/1‑149 (MQ=255)
                                                                                                            cgagcgaCAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGCGCTCGCTg                                  >  7:277771/1‑145 (MQ=255)
                                                                                                                 gaCAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGGGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGCGCTCGCTGCAGAGGGa                          >  2:61828/1‑148 (MQ=255)
                                                                                                                  gcggcGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGCGCTCGCTGCAGATGGaaa                        <  1:319439/145‑1 (MQ=255)
                                                                                                                  aCAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAg                                                 >  4:76102/1‑124 (MQ=255)
                                                                                                                  aCAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAg                                                 <  3:76102/124‑1 (MQ=255)
                                                                                                                     aCGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGCGCTCGCTGCAGATGGAAatt                      >  5:275870/1‑148 (MQ=255)
                                                                                                                      cGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGCGCTCGCTGCAGATGGAAattga                    <  2:321899/149‑1 (MQ=255)
                                                                                                                       gCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGCGCTCGCTGCAGATGGAAattgat                   <  1:308660/149‑1 (MQ=255)
                                                                                                                           cGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGCGCTCGCTGCAGATGGAAATTGATTGTc               >  8:140552/1‑149 (MQ=255)
                                                                                                                             aCGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGCGCTCGCTGCAGATGGAAat                       >  2:76155/1‑139 (MQ=255)
                                                                                                                             aCGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGCGCTCGCTGCAGATGGAAat                       <  1:76155/139‑1 (MQ=255)
                                                                                                                              ttccagcGGCTGCCCGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGCGCTCGCTGCAGATg                            >  6:314160/7‑133 (MQ=255)
                                                                                                                              ttccagcGGCTGCCCGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGCGCTCGCTGCAGATg                            <  5:314160/127‑1 (MQ=255)
                                                                                                                                tGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGCGCTCGCTGCAGATGGAAATTGATTGTCCGCTg          <  8:277771/149‑1 (MQ=255)
                                                                                                                                 gCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGCGCTCGCTGCAGATGGAAattg                     >  3:160336/1‑137 (MQ=255)
                                                                                                                                 gCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGCGCTCGCTGCAGATGGAAattg                     <  4:160336/137‑1 (MQ=255)
                                                                                                                                   gcGGCTGCCCGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGCGCTCGCTGCAGATGGAAATTGATTGTCCGCTGGcc       >  3:260110/2‑149 (MQ=255)
                                                                                                                                       cTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGCGCTCGCTGCAGATGGAAATTGATTGTCCGCTGGCCTTGa   <  3:294931/149‑1 (MQ=255)
                                                                                                                                        tGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGCGCTCGCTGCAGATGGAAATTGATTGTCCGCTGGCCTTGAc  <  3:315716/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                
CTGCTGCGCTTGCAGATGAACCAGGTCGATGCGCCGC‑GGGTGCTGCTGGCGGGTACCGAGGGTCAAGCCGAATGCCAGGTGTTGTGCGCCGGGCTGCTGTTGAGCAGCGAGCGACAACGCATCGACGTGCTCGGCTGCGGGCAGCCGCTGGAAGAGCTCCCGCTGCTGTGTGCAGCGGTGCAGCCTGCGGCACTGGTGATCGCGCTGCAGCCACCGGTGAGCCCTGCCTTGGCCAAGCGCCTGCGCTCGCTGCAGATGGAAATTGATTGTCCGCTGGCCTTGAC  >  NC_002947/3123046‑3123329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑