Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,150,455 G→A 8.7% G187R (GGG→AGG)  PP_2766 → long‑chain‑fatty acid‑‑CoA ligase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,150,4550GA 8.7% 126.8 / 2.9 46G187R (GGG→AGG) PP_2766long‑chain‑fatty acid‑‑CoA ligase‑like protein
Reads supporting (aligned to +/- strand):  ref base G (24/18);  new base A (2/2);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

CGTGGCCTGGCGGATTCCGGGTTGCCGAATTACGCTCGCCTGGCCGCGCAGCTGCCCGATCCTGCCTTGGCGGTGAAGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTGGCCTCCCGCAACTTCGCCACCAGCGGCCAGGCCCGCTACCTGCTGGCGCCTTGGCTGCTGGCAGG  >  NC_002947/3150311‑3150600
                                                                                                                                                |                                                                                                                                                 
cGTGGCCTGGCGGATTCCGGGTTGCCGAATTACGCTCGCCTGGCCGCGCAGCTGCCCGATCCTGCCTTGGCGGTGAAGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCt                                                                                                                                               >  5:110256/1‑149 (MQ=255)
cGTGGCCTGGCGGAATCCGGGTTGCCGAAATACGCACGCCTGGCCGCGCAGCTGCCCGATCCTGCCTTGGCGGTGAAGCCGCAGAGCGAGCGCCCAGCGTTCAGCTAACACCGGCTGGATGCCGATGGCCAACTTCCGCAGCACGGGc                                                                                                                                                >  8:211422/1‑148 (MQ=255)
         gCGGATTCCGGGTTGCCGAATTACGCTCGCCTGGCCGCGCAGCTGCCCGATCCTGCCTTGGCGGTGAAGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCCCCGGCTGGATGCCGATGGCCACCTTCAGCAGCACGGGCTCAGCCATGc                                                                                                                                      >  8:165497/1‑149 (MQ=255)
                  gggTTGCCGAATTACGCTCGCCTGGCCGCGCAGCTGCCCGTTCCTGCCTTGGCGGTGAAGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTGACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCt                                                                                                                             <  1:282321/149‑1 (MQ=255)
                   ggTTGCCGAATTACGCTCGCCTGGCCGCGCAGCTGCCCGATCCTGCCTTGGCGGTGAAGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTg                                                                                                                            >  3:276671/1‑149 (MQ=255)
                        ccGAATTACGCTCGCCTGGCCGCGCAGCTGCCCGATCCTGCCTTGGCGGTGAAGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGCTGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGc                                                                                                                         >  5:320551/1‑147 (MQ=255)
                           aaTTACGCTCGCCTGGCCGCGCAGCTGCCCGATCCTGCCTTGGCGGTGAAGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAggg                                                                                                                    <  6:317707/149‑1 (MQ=255)
                             ttACGCTCGCCTGGCCGCGCAGCTGCCCGATCCTGCCTTGGCGGTGAAGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGc                                                                                                                  <  8:271528/149‑1 (MQ=255)
                                     gCCTGGCCGCGCAGCTGCCCGATCCTGCCTTGGCGGTGAAGGCGCAGAGCGAGCGCCCAGCGTGCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTg                                                                                                          <  7:165497/149‑1 (MQ=255)
                                        tGGCCGCGGAGCTGCCCGATCCTGCCTTGGCGGTGATGGCGCAGATCGAGCGGCCAGCGTTCAGCGTTCATCGGCTGGATGCCGATGGCCTACTTCAGCAGCACGGGCGCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCAGGTc                                                                                                       <  7:211422/149‑1 (MQ=255)
                                        tGGCCGCGCAGCTGCCCGATCCTGCCTTGGCGGTGAAGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACAGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTc                                                                                                       >  3:243798/1‑149 (MQ=255)
                                        tGGCCGCGCAGCTGCCCGATCCTGCCTTGGCGGTGAAGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACAGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTc                                                                                                       >  3:248240/1‑149 (MQ=255)
                                          gCCGCGCAGCTGCCCGATCCTGCCTTGGCGGTGAAGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATACCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCg                                                                                                      <  7:301111/148‑1 (MQ=255)
                                          gCCGCGCAGCTGCCCGATCCTGCCTTGGCGGTGAAGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCGGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCg                                                                                                      >  8:206454/1‑148 (MQ=255)
                                            cgcgCAGCTGCCCGATCCTGCCTTGGCGGTGAAGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGaa                                                                                                    >  5:40493/1‑148 (MQ=255)
                                                    tGCCCGATCCTGCCTTGGCGGTGAAGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAgc                                                                                             >  3:297033/1‑147 (MQ=255)
                                                    tGCCCGATCCTGCCTTGGCGGTGAAGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAgc                                                                                             <  4:297033/147‑1 (MQ=255)
                                                              tGCCTTGGCGGTGAAGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGAcggcgg                                                                                 >  3:163685/1‑149 (MQ=255)
                                                                    ggCGGTGAAGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGa                                                                             <  4:240309/147‑1 (MQ=255)
                                                                      cGGTGAAGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGaa                                                                         <  7:206454/149‑1 (MQ=255)
                                                                            aGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTg                                                                   <  4:276671/149‑1 (MQ=255)
                                                                            aGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTg                                                                   <  6:110256/149‑1 (MQ=255)
                                                                            aGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACAGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTg                                                                   <  4:243798/149‑1 (MQ=255)
                                                                            aGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACAGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTg                                                                   <  4:248240/149‑1 (MQ=255)
                                                                             ggCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTgg                                                                  <  1:134354/149‑1 (MQ=255)
                                                                                        agcgCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGa                                                                             <  3:149933/127‑1 (MQ=255)
                                                                                        agcgCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGa                                                                             >  4:149933/1‑127 (MQ=255)
                                                                                                      aGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTGGCCTCCCGCAACTTCGCCACCAGCgg                                         >  2:276117/1‑149 (MQ=255)
                                                                                                       gCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTGGCCTCCCGCAACTTCGCCACCAGCGGc                                        >  2:44579/1‑149 (MQ=255)
                                                                                                        cTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTGGCCTCCCGCAACTTCGCCACCAGCGGc                                        >  6:113222/1‑148 (MQ=255)
                                                                                                         tttCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATTCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAGCAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTGGCCTCCCGCAACTTCGc                                                  <  8:172011/137‑1 (MQ=255)
                                                                                                         tttCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAGCAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTGGCCTCCCGCAACTTCGc                                                  >  7:172011/1‑137 (MQ=255)
                                                                                                         tttCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTGGCCTCCCGCAACTTCGCCACCAGCGGc                                        >  2:5193/1‑147 (MQ=255)
                                                                                                           tCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTgg                                                                  <  7:186605/119‑1 (MQ=255)
                                                                                                           tCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTgg                                                                  >  8:186605/1‑119 (MQ=255)
                                                                                                               cGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTGGCCTCCCGCAACTTCGCCACCAGCGGCCAGGCCCg                                >  1:169656/1‑149 (MQ=255)
                                                                                                                       tGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTGGCCTCCCGCAACTTCGCCACCAGCGGCCAGGCCCGCTACctgc                        <  1:44579/149‑1 (MQ=255)
                                                                                                                           gATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGATGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTGGCCTCCCGCAACTTCGCCACCAGCGGCCAGGCCCGCTACCTGCTGgc                    <  7:17897/149‑1 (MQ=255)
                                                                                                                                 cAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTGGCCTCCCGCAACTTCGCCACCAGCGGCCAGGCCCGCTACCTGCTGGCGccaag              >  7:176527/1‑146 (MQ=255)
                                                                                                                                 cAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTGGCCTCCCGCAACTTCGCCACCAGCGGCCAGGCCCGCTACCTGCTGGCGCCTTg              >  7:239/1‑149 (MQ=255)
                                                                                                                                 cAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTGGCCTCCCGCAACTTCGCCACCAGCGGCCAGGCCCGCTACCTGCTGGCGCCTTg              >  5:188573/1‑149 (MQ=255)
                                                                                                                                          cagcaCGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTGGCCTCCCGCAACTTCGCCACCAGCGGCCAGGCCCGCTACCTGCTGGCGCCTTGGCTGCTgg      >  2:166421/1‑148 (MQ=255)
                                                                                                                                            gcaCGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTGGCCTCCCGCAACTTCGCCACCAGCGGCCAGGCCCGCTACCTGCTGGCGCCTTGGCTGCTGGCAg   >  2:104450/1‑149 (MQ=255)
                                                                                                                                             caCGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTGGCCTCCCGCAACTTCGCCACCAGCGGCCAGGCCCGCTACCTGCTGGCGCCTTGGCTGCTGGc     <  3:33321/146‑1 (MQ=255)
                                                                                                                                             caCGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTGGCCTCCCGCAACTTCGCCACCAGCGGCCAGGCCCGCTACCTGCTGGCGCCTTGGCTGCTGGc     >  4:33321/1‑146 (MQ=255)
                                                                                                                                             caCGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTGGCCTCCCGCAACTTCGCCACCAGCGGCCAGGCCCGCTACCTGCTGGCGCCTTGGCTGCTGGCAgg  >  7:289778/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CGTGGCCTGGCGGATTCCGGGTTGCCGAATTACGCTCGCCTGGCCGCGCAGCTGCCCGATCCTGCCTTGGCGGTGAAGGCGCAGAGCGAGCGCCCAGCGTTCAGCTTTCACCGGCTGGATGCCGATGGCCAACTTCAGCAGCACGGGCTCAGCCATGCCGAGGTGCTGGGCGAGGGGCGTCGGCTGGTCGAACAGGAGCGCCTGACGGCGGCCGAGGAAGCCTTGGCCTCCCGCAACTTCGCCACCAGCGGCCAGGCCCGCTACCTGCTGGCGCCTTGGCTGCTGGCAGG  >  NC_002947/3150311‑3150600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑