Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,160,096 T→C 7.7% F4L (TTC→CTC)  PP_2775 → glycine betaine/L‑proline ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,160,0960TC 7.7% 145.5 / 3.2 52F4L (TTC→CTC) PP_2775glycine betaine/L‑proline ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (22/26);  new base C (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.40e-01

TGACCGCCTTGCGCAACATCAAGGGTGGTGAGGGCGTCCTGGCGGGTATTGCCATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGACAAGATCGTTATTGGCGAGCAGAACTGGACCGGCGCCATCGCCATCCAGTACATCCTCGGCGACATCATTTC  >  NC_002947/3159948‑3160241
                                                                                                                                                    |                                                                                                                                                 
tGACCGCCTTGCGCAACATCAAGGGTGGTGAGGGCGTCCTGGGGGGTATTGCCATTGTTTTTTGTGCCATGGTGCCCGACCGGATCATTCAGGAATCCCTTCGAAAACGTCATATTTCTACTCAGGAGTAACAGGTTCAATGACCCGAt                                                                                                                                                   >  5:332888/1‑149 (MQ=255)
  aCCGCCTTGCGCAACATCAAGGGTGGTGAGGGCGTCCTGGCGGGTATTGCCATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTc                                                                                                                                                 >  6:220891/1‑149 (MQ=255)
   ccGCCTTGCGCAACATCAAGGGTGGTGAGGGCGTCCTGGCGGGTATTGCCATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTcc                                                                                                                                                >  8:141566/1‑149 (MQ=255)
            gcAACATCAAGGGTGGTGAGGGCGTCCTGGCGGGTATTGCCATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCcgcg                                                                                                                                       <  2:203147/149‑1 (MQ=255)
              aaCATCAAGGGTGGTGAGGGCGTCCTGGCGGGTATTGCCATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCggg                                                                                                                                     <  7:243549/149‑1 (MQ=255)
                 aTCAAGGGTGGTGAGGGCGTCCTGGCGGGTATTGCCATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCtt                                                                                                                                  >  6:289979/1‑149 (MQ=255)
                    aaGGGTGGTGAGGGCGTCCTGGCGGGTATTGCCATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGc                                                                                                                                <  7:141566/148‑1 (MQ=255)
                     aGGGTGGTGAGGGCGTCCTGGCGGGTATTGCCATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGTCg                                                                                                                              <  2:57561/149‑1 (MQ=255)
                       ggtggtGAGGGCGTCCTGGCGGGTATTGCCATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCgg                                                                                                                             <  6:257068/148‑1 (MQ=255)
                       ggtggtGAGGGCGTCCTGGCGGGTATTGCCATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCgg                                                                                                                             >  5:257068/1‑148 (MQ=255)
                           gtgAGGGCGTCCTGGCGGGTATTGCCATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCg                                                                                                                        <  7:298041/149‑1 (MQ=255)
                             gAGGGCGTCCTGGCGGGTATTGCCATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGc                                                                                                                       >  2:314849/1‑148 (MQ=255)
                                ggCGTCCTGGCGGGTATTGCCATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTGCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTctg                                                                                                                   <  5:220891/149‑1 (MQ=255)
                                 gCGTCCTGGCGGGTATTGCCATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTctgc                                                                                                                  <  5:187587/149‑1 (MQ=255)
                                   gTCCTGGCGGGTATTGCCATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTctgctg                                                                                                                <  3:88377/149‑1 (MQ=255)
                                     ccTGGCGGGTATTGCCATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGctc                                                                                                                     <  7:18384/142‑1 (MQ=255)
                                     ccTGGCGGGTATTGCCATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGctc                                                                                                                     >  8:18384/1‑142 (MQ=255)
                                         gCGGGTATTGCCATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCcgc                                                                                                           <  6:246908/148‑1 (MQ=255)
                                         gCGGGTATTGCCATTGTTTTTTGTGCCCTCGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCcgcg                                                                                                          >  7:192221/1‑149 (MQ=255)
                                                   ccATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCgg                                                                                                >  6:298667/1‑149 (MQ=255)
                                                   caaTTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCgg                                                                                                >  1:137708/3‑149 (MQ=255)
                                                    cATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGc                                                                                               >  3:160332/1‑149 (MQ=255)
                                                      ttGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGa                                                                                             <  5:298667/149‑1 (MQ=255)
                                                        gTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGAc                                                                                            <  1:314849/148‑1 (MQ=255)
                                                         ttttttGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAg                                                                                          >  3:14148/1‑149 (MQ=255)
                                                            tttGTGGCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGCCCCGATTCGTTTCCCGCGGGCTGGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCa                                                                                        <  5:95203/148‑1 (MQ=255)
                                                            tttGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCa                                                                                        <  1:108690/148‑1 (MQ=255)
                                                                  cccTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAg                                                                                      <  5:9689/144‑1 (MQ=255)
                                                                       tctgccGACCGGATCATTCTGTGATCCCTTCGCATACGTCATCTTTCTAGTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCGGCGGGCGTGCCGGGTTCGGTCTGCTGGCCGCGCTGGTACCGGCGACAGCTAGCTTCGCCaa                                                                             <  6:60573/144‑1 (MQ=255)
                                                                       gTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAg                                                                            >  3:130564/1‑149 (MQ=255)
                                                                           ccGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGACa                                                                        <  5:289979/149‑1 (MQ=255)
                                                                             gACCGGATCGTTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGACTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGAc                                                                         >  3:208006/1‑146 (MQ=255)
                                                                             gACCGGATCGTTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGACTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGAc                                                                         >  3:203922/1‑146 (MQ=255)
                                                                             gACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGAc                                                                         >  1:284177/1‑146 (MQ=255)
                                                                                    tccttCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGACTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGACAAGATCGTTa                                                               <  4:203922/146‑1 (MQ=255)
                                                                                    tccttCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGACTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGACAAGATCGTTa                                                               <  4:208006/146‑1 (MQ=255)
                                                                                      aTTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGACAAGATCGTTAtt                                                             <  2:137708/149‑1 (MQ=255)
                                                                                      aTTCAGGCATCCCTTAGCAAACGTCATCATTCTACTAAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGTTACCGGCGACAGCAAGCTTCGCCAAGGACAAGATCGTTAtt                                                             <  6:332888/149‑1 (MQ=255)
                                                                                          aGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGACAAGATCGTTATTGGc                                                          >  8:11155/1‑148 (MQ=255)
                                                                                            gCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTc                                                                                  <  7:104334/122‑1 (MQ=255)
                                                                                            gCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTc                                                                                  >  8:104334/1‑122 (MQ=255)
                                                                                                            gTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGACAAGATCGTTATTGGCGAGCAGAACTGGACCg                                          >  2:318360/1‑146 (MQ=255)
                                                                                                            gTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGACAAGATCGTTATTGGCGAGCAGAACTGGACCg                                          <  1:318360/146‑1 (MQ=255)
                                                                                                               atcatTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGACAAGAGCGTTATTGGCGAGCAGAACTGGACCGGcgcca                                    >  4:193275/1‑149 (MQ=255)
                                                                                                                  atTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGACAAGATCGTTATTGGCGAGCAGAACTGGACCGGcgccatcg                                 >  6:265942/1‑149 (MQ=255)
                                                                                                                     cTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGACAAGATCGTTATTGGCGAGCAGAACTGGACCGGcgccatcgcc                               <  8:192221/148‑1 (MQ=255)
                                                                                                                      tACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGACAAGATCGTTATTGGCGAGCAGaa                                                  <  1:71373/128‑1 (MQ=255)
                                                                                                                      tACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGACAAGATCGTTATTGGCGAGCAGaa                                                  >  2:71373/1‑128 (MQ=255)
                                                                                                                           aGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGACAAGATCGTTATTGGCGAGCAGAACTGGACCGGCGCCATCGCCATCCAg                         >  7:70246/1‑148 (MQ=255)
                                                                                                                            ggAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGACAAGATCGTTATTGGCGAGCAGAACTGGACCGGCGCCATCGCCATCCAGTa                       <  4:130564/149‑1 (MQ=255)
                                                                                                                                  aCAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGACAAGATCGTTATTGGCGAGCAGAACTGGACCGGCGCCATCGCCATCCAGTACATCCt                 >  7:183085/1‑149 (MQ=255)
                                                                                                                                               cccGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGACAAGATCGTTATTGGCGAGCAGAACTGGACCGGCGCCATCGCCATCCAGTACATCCTCGGCGACATCAtt    >  8:141821/1‑149 (MQ=255)
                                                                                                                                                 cGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGACAAGATCGTTATTGGCGAGCAGAACTGGACCGGCGCCATCGCCATCCAGTACATCCTCGGCGACATCATTTc  <  2:284177/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TGACCGCCTTGCGCAACATCAAGGGTGGTGAGGGCGTCCTGGCGGGTATTGCCATTGTTTTTTGTGCCCTGGTGCCCGACCGGATCATTCAGGCATCCCTTCGCAAACGTCATCATTCTACTCAGGAGTAACAGGTTCCATGACCCGATTCCTTTCCCGCGGGCTTGCCGGGTTCGCTCTGCTGGCCGCGCTGGTACCGGCGACAGCAAGCTTCGCCAAGGACAAGATCGTTATTGGCGAGCAGAACTGGACCGGCGCCATCGCCATCCAGTACATCCTCGGCGACATCATTTC  >  NC_002947/3159948‑3160241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑