Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,189,278	G→C	10.2%	L458V (CTG→GTG)	PP_2799 ←	class III aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,189,278	0	G	C	10.2%	170.5 / 10.3	59	L458V (CTG→GTG)	PP_2799	class III aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (29/24);  new base C (3/3);  total (32/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

TGGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATCAGCCCATGCTTGT  >  NC_002947/3189144‑3189424                                                                                                                                        |                                                                                                                                                   tgGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAgcgcg                                                                                                                                      >  1:9333/1‑149 (MQ=255)  ggTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTTTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTCGCTCACAGCACCTGCTGTGAgcaaa                                                                                                                                      <  8:170989/148‑4 (MQ=255)  ggTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCACCTGCTGTGAg                                                                                                                                          >  2:251196/1‑144 (MQ=255)  ggTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGCTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCACCTGCTGTGAg                                                                                                                                          <  1:251196/144‑1 (MQ=255)   gTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGcc                                                                                                                                    >  2:167349/1‑149 (MQ=255)       ccATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGTGCGCCCAAt                                                                                                                                >  6:285114/1‑149 (MQ=255)         aTGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATcc                                                                                                                              >  3:135369/1‑149 (MQ=255)           ggCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAGTACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCg                                                                                                                             >  1:98932/1‑149 (MQ=255)            gCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTc                                                                                                                           <  8:64871/149‑1 (MQ=255)              aCCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTc                                                                                                                           >  4:186777/1‑147 (MQ=255)              aCCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTc                                                                                                                           <  3:186777/147‑1 (MQ=255)                    cccGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCtgt                                                                                                                                             <  2:215521/123‑1 (MQ=255)                               aaaCGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCt                                                                                                        >  8:35933/1‑149 (MQ=255)                                        aCTCGAAGCTTTCATCACTGTCTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGgcgc                                                                                               >  4:303813/1‑149 (MQ=255)                                          tCGAAGCTTTCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCATAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCt                                                                                              >  4:212952/1‑148 (MQ=255)                                            gAAGCTTTCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTg                                                                                             >  2:232306/1‑147 (MQ=255)                                            gAAGCTTTCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTg                                                                                             >  2:107976/1‑147 (MQ=255)                                            gAAGCTTTCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATCCAGCCTTTGCTCACAGAAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTg                                                                                             >  5:192069/1‑147 (MQ=255)                                            gAAGCTTTCATCACTGTGTTCACGCACGGTAA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGaa                                                                                           <  4:90578/149‑1 (MQ=255)                                              aGCTTTCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGa                                                                                         <  1:96971/149‑1 (MQ=255)                                                cTTTCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATc                                                                                       <  2:9333/149‑1 (MQ=255)                                                 tttCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCg                                                                                      >  4:218622/1‑149 (MQ=255)                                                  ttCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGc                                                                                     >  2:159921/1‑149 (MQ=255)                                                    catcaCTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGTAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCt                                                                                   >  8:151276/1‑149 (MQ=255)                                                       caCTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGa                                                                                >  5:326326/1‑149 (MQ=255)                                                          tgtgTTCACGCACGGTCA‑AACCCCCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGatat                                                                             >  6:274084/1‑149 (MQ=255)                                                            tgtTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGTGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATc                                                                            <  5:285114/148‑1 (MQ=255)                                                               tCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGa                                                                                <  4:20694/141‑1 (MQ=255)                                                               tCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGa                                                                                >  3:20694/1‑141 (MQ=255)                                                                  cgcacgGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTgg                                                                     <  7:284286/149‑1 (MQ=255)                                                                  cgcacgGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTgg                                                                     <  1:232306/149‑1 (MQ=255)                                                                         tCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTaa                                                              >  8:233136/1‑149 (MQ=255)                                                                         tCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTaa                                                              >  8:237511/1‑149 (MQ=255)                                                                           a‑aaCCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGTAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAAt                                                             <  7:151276/148‑1 (MQ=255)                                                                             aaCCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCa                                                           <  5:141509/149‑1 (MQ=255)                                                                                ccTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACg                                                        <  1:159921/149‑1 (MQ=255)                                                                                 cttatttttgttgTTTTAGGTGAGTAGCGATAAGTTGAAGCGTTTGCTCACCGATGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACgg                                                       <  1:167349/142‑1 (MQ=255)                                                                                  tCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCt                                                                       >  6:236585/1‑132 (MQ=255)                                                                                  tCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCt                                                                       <  5:236585/132‑1 (MQ=255)                                                                                  tCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACg                                                        >  8:31470/1‑147 (MQ=255)                                                                                   cATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACggag                                                     >  6:210149/1‑149 (MQ=255)                                                                                    aTGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACggag                                                     <  7:233136/148‑1 (MQ=255)                                                                                    aTGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACggag                                                     <  7:237511/148‑1 (MQ=255)                                                                                       aTTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGAc                                                  <  6:326326/148‑1 (MQ=255)                                                                                           ttgttTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGaa                                              <  7:35933/148‑1 (MQ=255)                                                                                                  aCGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGa                                                                                >  7:15095/1‑107 (MQ=255)                                                                                                  aCGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGa                                                                                <  8:15095/107‑1 (MQ=255)                                                                                                    gTGAGTAGCCATCAGATGCAGCCTTTGCTCATAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCgtg                                    <  3:212952/149‑1 (MQ=255)                                                                                                       aGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTc                                  <  6:192069/148‑1 (MQ=255)                                                                                                            ccATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATACGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTGGTAGAt                            >  2:32778/1‑149 (MQ=255)                                                                                                                cAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGgcg                        >  4:216836/1‑149 (MQ=255)                                                                                                                  gATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGgcgc                       >  8:87434/1‑148 (MQ=255)                                                                                                                    tGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGaa                    >  4:154800/1‑149 (MQ=255)                                                                                                                         ccTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATc               <  7:87434/149‑1 (MQ=255)                                                                                                                            gcgCTCACAGCACCTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATCAGc            >  3:200460/3‑149 (MQ=255)                                                                                                                             tGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATCAg             <  2:98932/147‑1 (MQ=255)                                                                                                                              gCTCACAGCACCTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCgt                                     <  4:12381/122‑1 (MQ=255)                                                                                                                              gCTCACAGCACCTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCgt                                     >  3:12381/1‑122 (MQ=255)                                                                                                                               cTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATCAGCCCa         >  5:83973/1‑149 (MQ=255)                                                                                                                                      cagGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATCAGCCCATGCTtgt  <  6:83973/149‑1 (MQ=255)                                                                                                                                       agGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCTATCGTGTCGTAGATGGCGCGAATGATCAGCCCATGCTtgt  <  3:303813/148‑1 (MQ=255)                                                                                                                                        |                                                                                                                                                   TGGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCA‑AACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATCAGCCCATGCTTGT  >  NC_002947/3189144‑3189424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑