Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,261,795 A→G 8.0% Y111H (TAC→CAC)  PP_2859 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,261,7950AG 8.0% 139.4 / 3.2 50Y111H (TAC→CAC) PP_2859hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (24/22);  new base G (2/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

ATTTGGTGCGGTTGATTGTGCCGCCTCAGCCACGCCTGGCTGTCGTCGAAAAAGCGCTGACGATCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTCCAGAATTAGCAGCGGGGGCGCCTGCTCCCGCGCACGGTCCTCCCATGCCAGTTGT  >  NC_002947/3261653‑3261940
                                                                                                                                               |                                                                                                                                                  
aTTTGGTGCGGTTGATTGTGCCGCCTCAGCCACGCCTGGCTGTCGTCGAAAAAGCGCTGACGATCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCg                                                                                                                                              >  3:272522/1‑149 (MQ=255)
               ttGTGCCGCCTCAGCCACGCCTGGCTGTCGTCGAAAAAGCGCTGACGATCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTGACGCCGTGCCAGCAGTGCctc                                                                                                                               >  6:6683/1‑149 (MQ=255)
               ttGTGCCGCCTCAGCCACGCCTGGCTGTCGTCGAAAAAGCGCTGACGATCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTGACGCCGTGCCAGCAGTGCctc                                                                                                                               >  6:1970/1‑149 (MQ=255)
                  tgCCGCCTCAGCCACGCCTGGCTGTCGTCGAAAAAGCGCTGACGATCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTc                                                                                                                            >  1:317127/1‑149 (MQ=255)
                    ccgccTCAGCCACGCCTGGCTGTCGTCGAAAAAGCGCTGACGATCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTcca                                                                                                                          <  4:272522/149‑1 (MQ=255)
                            gCCACGCCTGGCTGTCGTCGAAAAAGCGCTGACGATCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCACCAGGTGGAGGTCGTAACGCCGTCCCAGCAGTGCCTCTTCCAGCCAggat                                                                                                                  >  4:287864/1‑147 (MQ=255)
                             ccACGCCTGGCTGTCGTCGAAAAAGCGCTGACGATCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTg                                                                                                                >  3:143476/1‑149 (MQ=255)
                              cACGCCTGGCTGTCGTCGAAAAAGCGCTGACGATCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTgg                                                                                                               >  3:137464/1‑149 (MQ=255)
                                cGCCTGGCTGTCGTCGAAAAAGCGCTGACGATCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTGACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGc                                                                                                             <  5:1970/149‑1 (MQ=255)
                                cGCCTGGCTGTCGTCGAAAAAGCGCTGACGATCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTGACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGc                                                                                                             <  5:6683/149‑1 (MQ=255)
                                   cTGGCTGTCGTCGAAAAAGCGCTGACGATCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGt                                                                                                          >  4:271512/1‑149 (MQ=255)
                                        tGTCGTCGAAAAAGCGCTGACGATCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGcc                                                                                                      >  4:6819/1‑148 (MQ=255)
                                          tcttcgTAAAAGGGCTGACGATGACTGTGGTCGGGTTGGCTACGCTGCCCATCGGCAAT‑CCTGTCCACCCCTTGAGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGaa                                                                                                   <  7:143161/146‑1 (MQ=255)
                                                 aaaaGCGCTGACGATCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAATGCCAGACCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGc                                                                                             >  1:251435/1‑149 (MQ=255)
                                                    aGCGCTGACGATCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCTAACAGTGCgggg                                                                                         <  4:137464/149‑1 (MQ=255)
                                                      cgcTGACGATCACTGAGGTCGGGTTGGCAACGCTGCCGATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAAGAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCt                                                                                       <  3:287864/149‑1 (MQ=255)
                                                      cgcTGACGATCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCcagc                                                                                                                                       >  2:54624/1‑102 (MQ=255)
                                                      cgcTGACGATCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCcagc                                                                                                                                       <  1:54624/102‑1 (MQ=255)
                                                           aCGATCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCaga                                                                                  <  3:271512/149‑1 (MQ=255)
                                                             gATCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCagag                                                                                 >  7:141017/1‑148 (MQ=255)
                                                              aTCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGACCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGGTTTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGc                                                                                <  1:211487/149‑1 (MQ=255)
                                                                cACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATg                                                                             >  2:219549/1‑149 (MQ=255)
                                                                 aCTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGt                                                                            >  2:139961/1‑149 (MQ=255)
                                                                  cTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGtt                                                                           <  4:143476/149‑1 (MQ=255)
                                                                     aGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTg                                                                          <  2:251435/147‑1 (MQ=255)
                                                                              tGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCTGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAggtg                                                               <  2:317127/149‑1 (MQ=255)
                                                                                 cAACGCTGCCCAACGGCAAA‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCCTGCCAGCAGAGCCTCTTCCAGCCAGG‑TAGGGCAGTCGCCGAACAGTGACAGGATCCAGAGCATGTTGCTTAGCAggtgggt                                                            >  8:165709/1‑149 (MQ=255)
                                                                                  aaCGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTa                                                                      >  8:181979/1‑138 (MQ=255)
                                                                                  aaCGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTa                                                                      <  7:181979/138‑1 (MQ=255)
                                                                                     gCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTcc                                                        <  1:139961/149‑1 (MQ=255)
                                                                                           cATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTCCAGAAtt                                                  <  3:203859/149‑1 (MQ=255)
                                                                                            aTCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTCCAGAAt                                                   >  4:25183/1‑147 (MQ=255)
                                                                                             tCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGt                                                                            <  4:102262/121‑1 (MQ=255)
                                                                                             tCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGt                                                                            >  3:102262/1‑121 (MQ=255)
                                                                                                 cAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTCCAGAATTagcagc                                            >  4:129095/1‑149 (MQ=255)
                                                                                                    t‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTCCAGAATTAGCAGCggg                                         <  6:207676/149‑1 (MQ=255)
                                                                                                               cccTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTCCAGAATTAGCAGCGGGGGCGCCTGCt                               <  8:272429/149‑1 (MQ=255)
                                                                                                                  ttGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTCCAGAATTAGCAGCGGGGGCGCCTGCTccc                            >  5:205976/1‑149 (MQ=255)
                                                                                                                       ggCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTCCAGAATTAGCAGCGGGGGCGCCTGCTCCCGCGCa                       <  3:6819/149‑1 (MQ=255)
                                                                                                                        gCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTCCAGAATTAGCAGCGGGGGCGCCTGCTCCCGCGCAc                      >  7:141347/1‑149 (MQ=255)
                                                                                                                               aaCAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTCCAGAATTAGCAGCGGGGGCGCCTGCTCCCGCGCACGGtcctc               >  8:275806/1‑149 (MQ=255)
                                                                                                                                  aGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTCCAGAATTAGCAGCGGGGGCGCCTGCTCCCGCGCACGGTCCTccc             >  6:192645/1‑148 (MQ=255)
                                                                                                                                   ggTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTCCAGAATTAGCAGCGGGGGCGCCTGCTCCCGCGCACGGtcctcc              >  3:51684/1‑146 (MQ=255)
                                                                                                                                   ggTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTCCAGAATTAGCAGCGGGGGCGCCTGCTCCCGCGCACGGtcctcc              <  4:51684/146‑1 (MQ=255)
                                                                                                                                    gTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTCCAGAATTAGCAGCGGGGGCGCCTGCTCCCGCGCa                       <  2:41490/136‑1 (MQ=255)
                                                                                                                                    gTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTCCAGAATTAGCAGCGGGGGCGCCTGCTCCCGCGCa                       >  1:41490/1‑136 (MQ=255)
                                                                                                                                       gAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTCCAGAATTAGCAGCGGGGGCGCCTGCTCCCGCGCACGGTCCTCCCATGCCa       <  8:141017/149‑1 (MQ=255)
                                                                                                                                          gTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTCCAGAATTAGCAGCGGGGGCGCCTGCTCCCGCGCACGGtcctc               >  4:55026/1‑138 (MQ=255)
                                                                                                                                          gTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTCCAGAATTAGCAGCGGGGGCGCCTGCTCCCGCGCACGGtcctc               <  3:55026/138‑1 (MQ=255)
                                                                                                                                             gTAACGCCGTGCCAGGAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTCCAGAATTAGCAGCGGGGGCGCCTGCTCCCGCGCACGGTCCTCCCATGCCAGTTGt  <  6:205976/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
ATTTGGTGCGGTTGATTGTGCCGCCTCAGCCACGCCTGGCTGTCGTCGAAAAAGCGCTGACGATCACTGAGGTCGGGTTGGCAACGCTGCCCATCGGCAAT‑CCAGTCCACCCCTTGCGGGCTGAGCAACAGGTGGAGGTCGTAACGCCGTGCCAGCAGTGCCTCTTCCAGCCAGG‑TTGGGCAGTCGCCGAACAGTGCGTGGCTCCAGAGCATGTTGCTTAGCAGGTGGGTGTCCAGAATTAGCAGCGGGGGCGCCTGCTCCCGCGCACGGTCCTCCCATGCCAGTTGT  >  NC_002947/3261653‑3261940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑