Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,266,102 T→C 10.2% intergenic (+19/+40) PP_2863 → / ← PP_2864 membrane protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,266,1020TC10.2% 130.1 / 5.5 49intergenic (+19/+40)PP_2863/PP_2864membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (20/24);  new base C (2/3);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01

GCTGTTTCGGCACAAGACCCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCG‑‑ACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGG  >  NC_002947/3265960‑3266245
                                                                                                                                              |                                                                                                                                                 
gcTGTTTCGGCACAAGACCCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTcgc                                                                                                                                             <  3:123911/149‑1 (MQ=255)
  tGTTTCGGCACAAGACCCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCgg                                                                                                                                           >  1:143550/1‑149 (MQ=255)
   gTTTCGGCACAAGACCCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCCTTTCGCggc                                                                                                                                          >  2:14652/1‑148 (MQ=255)
             aaGACCCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCcgc                                                                                                                                 >  3:251782/1‑148 (MQ=255)
                 cccGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGCTTTACCCGCGaaga                                                                                                                            <  8:186815/149‑3 (MQ=255)
                    gCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCCTTTCGCGGCTTTACCCGCGaagaggc                                                                                                                         <  1:14652/149‑6 (MQ=255)
                      aaGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCCTTTCGCGGCTTTACCCGCGAAGAGGCCg                                                                                                                       <  8:31528/149‑1 (MQ=38)
                                 ccAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGa                                                                                                            >  6:171283/1‑149 (MQ=255)
                                  cAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGAt                                                                                                           >  4:61379/1‑149 (MQ=255)
                                   aGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCgg                                                                                                                                           <  6:125524/116‑1 (MQ=255)
                                   aGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCgg                                                                                                                                           >  5:125524/1‑116 (MQ=255)
                                        gggtgCTGGGGGATTGGGCTGCTGCACCAGGTGTTCTGCGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACAt                                                                                                      <  8:121263/146‑1 (MQ=255)
                                         tgtgCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCgg                                                                                                                                           <  1:264922/110‑1 (MQ=255)
                                         tgtgCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCgg                                                                                                                                           >  2:264922/1‑110 (MQ=255)
                                         tgtgCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGa                                                                                                    <  4:251782/149‑1 (MQ=255)
                                         tgtgCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGa                                                                                                    <  7:177656/149‑1 (MQ=255)
                                           tgCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGAc                                                                                                   <  5:171283/148‑1 (MQ=255)
                                            gCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGAcc                                                                                                  >  8:283047/1‑148 (MQ=255)
                                               gggggATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAAcc                                                                                              >  7:299468/1‑149 (MQ=255)
                                                ggggATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGAc                                                                                                   >  8:106302/1‑143 (MQ=255)
                                                ggggATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGAc                                                                                                   <  7:106302/143‑1 (MQ=255)
                                                          ctgctgcTCCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCaa                                                                                    <  2:143550/148‑1 (MQ=255)
                                                                cACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCtt                                                                              <  7:283047/148‑1 (MQ=255)
                                                                 aCCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTgg                                                                            <  8:299468/149‑1 (MQ=255)
                                                                     ggTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCgg                                                                                                                                           <  8:195097/82‑1 (MQ=255)
                                                                     ggTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCgg                                                                                                                                           >  7:195097/1‑82 (MQ=255)
                                                                      gtgtTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAAc                                                                       >  3:22479/1‑149 (MQ=255)
                                                                         ttCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGt                                                                                                                                         <  6:172763/80‑1 (MQ=255)
                                                                         ttCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGt                                                                                                                                         >  5:172763/1‑80 (MQ=255)
                                                                            tGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATc                                                                                                          >  8:33364/1‑108 (MQ=255)
                                                                              ggCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCG‑‑ACGCAc                                                             <  3:61379/149‑1 (MQ=255)
                                                                                     tGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCG‑‑ACGCACTACCAg                                                       >  6:195911/1‑148 (MQ=255)
                                                                                       gCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCG‑‑ACGCACTACCAg                                                       <  5:195911/146‑1 (MQ=255)
                                                                                       gCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCG‑‑ACGCACTACCAGCt                                                     <  4:22479/148‑1 (MQ=255)
                                                                                         tGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACa                                                                                                                  >  2:283086/1‑87 (MQ=255)
                                                                                         tGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACa                                                                                                                  <  1:283086/87‑1 (MQ=255)
                                                                                           tttCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCG‑‑ACGCACTACCAGCTGGTGc                                                >  2:259509/1‑149 (MQ=255)
                                                                                                tGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATc                                                                                                          >  4:247680/1‑88 (MQ=255)
                                                                                                  aCGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTCCCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTCGGCAACCG‑‑ACGCACTACCAGCTGGTTCGACCGCt                                         <  8:141053/149‑1 (MQ=255)
                                                                                                      gCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCG‑‑ACGCACTACCAGCTGGTGCGACCGCTGCaa                                     <  2:44993/149‑1 (MQ=255)
                                                                                                            tCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAAcccc                                                                                            >  2:273345/1‑90 (MQ=255)
                                                                                                                   cTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTggg                                                                           <  1:262924/100‑1 (MQ=255)
                                                                                                                   cTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTggg                                                                           >  2:262924/1‑100 (MQ=255)
                                                                                                                                   ttGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCG‑‑ACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATgg        <  1:269677/149‑1 (MQ=255)
                                                                                                                                   ttGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCG‑‑ACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATgg        >  2:269677/1‑149 (MQ=255)
                                                                                                                                     gttccGGCCCTTTCGCGGCTTTACCCGCGAAGAGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCG‑‑ACGCACTACCAGCTGGTg                                                 <  3:64802/103‑1 (MQ=21)
                                                                                                                                     gttccGGCCCTTTCGCGGCTTTACCCGCGAAGAGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCG‑‑ACGCACTACCAGCTGGTg                                                 >  4:64802/4‑106 (MQ=21)
                                                                                                                                      tACCCGCCTCATCGGCGGTAAACCCGCGTAATGGCCGGTAAATGTCGTATACATGTACAAACCCACCTTCAACCGCTTTGGCAAACGTCACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATgg        <  1:259509/148‑1 (MQ=255)
                                                                                                                                         cGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCG‑‑ACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAgg  <  2:252781/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
GCTGTTTCGGCACAAGACCCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCG‑‑ACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGG  >  NC_002947/3265960‑3266245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑