Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,273,655 G→A 8.8% A111T (GCA→ACA)  PP_2871 → class II aldolase/adducin domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,273,6550GA 8.8% 190.1 / 7.0 68A111T (GCA→ACA) PP_2871class II aldolase/adducin domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (33/29);  new base A (2/4);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01

TCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGCCGGTGATGATGC  >  NC_002947/3273512‑3273802
                                                                                                                                                |                                                                                                                                                   
gcGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTg                                                                                                                                                <  3:8057/148‑1 (MQ=255)
      tGCAGGGGCGGCCGGTGGGGGCTGCCAGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAAtgt                                                                                                                                          >  1:5073/1‑149 (MQ=255)
       gCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAAtgtg                                                                                                                                         >  5:251782/1‑149 (MQ=255)
        cAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAAtgtg                                                                                                                                         <  5:13864/148‑1 (MQ=255)
        cAGGGGAGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTgg                                                                                                                                        >  4:53954/1‑149 (MQ=255)
            ggcggcCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCTGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCACATTCCAATGCGGccac                                                                                                                                    <  6:69824/149‑3 (MQ=255)
             gcggcCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCTGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCACATTCAAATGCGGccacc                                                                                                                                   <  6:200070/149‑4 (MQ=255)
                 ccGGGGGGGGCGGCCTGTCAGCCGGTCAACCCGGCCGGGGTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGGGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGCAATGTGGCCGGCTGcc                                                                                                                               <  8:207578/149‑1 (MQ=255)
                   ggTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCgg                                                                                                                             <  2:217251/149‑1 (MQ=255)
                        gggCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAAc                                                                                                                        >  7:269368/1‑149 (MQ=255)
                          gCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAAc                                                                                                                        >  8:280083/1‑147 (MQ=255)
                                    aTCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGGTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATg                                                                                                            >  1:233026/1‑149 (MQ=255)
                                    aTCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATg                                                                                                            >  7:266527/1‑149 (MQ=255)
                                    aTCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCACATTCCAATGCGGccac                                                                                                                                    >  6:328370/1‑123 (MQ=255)
                                    aTCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCACATTCCAATGCGGccac                                                                                                                                    <  5:328370/125‑3 (MQ=255)
                                        ggTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGt                                                                                                        <  2:233026/149‑1 (MQ=255)
                                         gtaaaCCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCACATTCCAATGCGGcca                                                                                                                                     >  6:222005/4‑118 (MQ=255)
                                         gtaaaCCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCACATTCCAATGCGGcca                                                                                                                                     <  5:222005/116‑2 (MQ=255)
                                            aaCCCTGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGtttt                                                                                                     <  6:294284/148‑1 (MQ=255)
                                            aaCCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTa                                                                                                    >  7:10176/1‑149 (MQ=255)
                                            aaCCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTa                                                                                                    >  1:80115/1‑149 (MQ=255)
                                                        ttCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGc                                                                                        <  8:266527/149‑1 (MQ=255)
                                                               ttCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCAc                                                                                 >  7:140160/1‑149 (MQ=255)
                                                               ttCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCAc                                                                                 >  1:58908/1‑149 (MQ=255)
                                                                   caGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCAt                                                                                                             >  6:171337/1‑117 (MQ=255)
                                                                   caGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCAt                                                                                                             <  5:171337/117‑1 (MQ=255)
                                                                        ccATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGa                                                                         <  8:269368/148‑1 (MQ=255)
                                                                        ccATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAg                                                                        >  1:28811/1‑149 (MQ=255)
                                                                          aTTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAggg                                                                      >  8:254129/1‑149 (MQ=255)
                                                                          aTTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAggg                                                                      >  6:155459/1‑149 (MQ=255)
                                                                           ttCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGc                                                                     >  8:222224/1‑149 (MQ=255)
                                                                              aCGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTc                                                                  >  1:146663/1‑149 (MQ=255)
                                                                                    cccGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCgcgc                                                              <  8:140160/147‑1 (MQ=255)
                                                                                    cccGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTg                                                            <  3:53954/149‑1 (MQ=255)
                                                                                         gAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACAt                                                       <  2:58908/149‑1 (MQ=255)
                                                                                         gAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACAt                                                       >  2:302948/1‑149 (MQ=255)
                                                                                                 ccGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGaa                                                >  2:276775/1‑148 (MQ=255)
                                                                                                   ggTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAg                                             <  6:251782/149‑1 (MQ=255)
                                                                                                      gcgtgcTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCaa                                          <  2:28811/149‑1 (MQ=255)
                                                                                                              cacacccacaccCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCt                                                                             <  6:103073/106‑1 (MQ=255)
                                                                                                              cacacccacaccCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCt                                                                             >  5:103073/1‑106 (MQ=255)
                                                                                                                cacccacacccGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCtggtgg                                <  7:254129/149‑1 (MQ=255)
                                                                                                                  cccacacccGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAAc                                               >  3:132528/1‑132 (MQ=255)
                                                                                                                  cccacacccGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAAc                                               <  4:132528/132‑1 (MQ=255)
                                                                                                                   ccacacccGCGCAGGCTGCGCGGTGGACCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGAt                                                   >  1:3727/1‑128 (MQ=255)
                                                                                                                   ccacacccGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGAt                                                   <  2:3727/127‑1 (MQ=255)
                                                                                                                   ccacacccGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGcc                              >  1:121412/1‑148 (MQ=255)
                                                                                                                   ccacacccGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGcc                              >  1:116863/1‑148 (MQ=255)
                                                                                                                   ccacacccGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGcc                              >  4:173587/1‑148 (MQ=255)
                                                                                                                   ccacacccGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCg                             >  3:41236/1‑149 (MQ=255)
                                                                                                                   acacacccGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGCGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGcc                              >  6:271814/2‑148 (MQ=255)
                                                                                                                      ccaccGCGCAGCCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGAcc                          >  8:146730/4‑149 (MQ=255)
                                                                                                                          cgcgCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGa                                                    >  7:224052/1‑119 (MQ=255)
                                                                                                                          cgcgCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGa                                                    <  8:224052/119‑1 (MQ=255)
                                                                                                                          cgcgCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTggg                      <  2:5073/149‑1 (MQ=255)
                                                                                                                           gcgcAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGc                     <  7:280083/149‑1 (MQ=255)
                                                                                                                             gcAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGcaa                   <  2:80115/149‑1 (MQ=255)
                                                                                                                              cAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGcaac                  <  4:41236/149‑1 (MQ=255)
                                                                                                                                 gCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACg                                                                                                  <  1:140288/66‑1 (MQ=255)
                                                                                                                                 gCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAg               >  7:89973/1‑149 (MQ=255)
                                                                                                                                 gCTGAGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACg                                                                                                  >  2:140288/1‑66 (MQ=255)
                                                                                                                                   tGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGcc             >  8:210252/1‑149 (MQ=255)
                                                                                                                                    gcgcGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGCCg            <  8:10176/149‑1 (MQ=255)
                                                                                                                                     cgcgGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGCCgg           <  8:76419/149‑1 (MQ=255)
                                                                                                                                     cgcgGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGCCgg           <  6:291245/149‑1 (MQ=255)
                                                                                                                                     cgcgGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGCCgg           <  7:222224/149‑1 (MQ=255)
                                                                                                                                            g‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCTTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGCCGGtgatga     <  7:210252/148‑1 (MQ=255)
                                                                                                                                               cGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGCCGGTGATGAtgc  >  5:321365/1‑149 (MQ=255)
                                                                                                                                                gCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAg                                                                        >  6:190405/1‑78 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TCGACCTGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGCCGGTGATGATGC  >  NC_002947/3273512‑3273802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑