Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,273,666 T→C 8.9% C114C (TGT→TGC)  PP_2871 → class II aldolase/adducin domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,273,6660TC 8.9% 177.1 / 7.1 67C114C (TGT→TGC) PP_2871class II aldolase/adducin domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (33/28);  new base C (2/4);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

TGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGCCGGTGATGATGCTGCGCAACCATG  >  NC_002947/3273518‑3273814
                                                                                                                                                     |                                                                                                                                                    
tGCAGGGGCGGCCGGTGGGGGCTGCCAGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAAtgt                                                                                                                                                      >  1:5073/1‑149 (MQ=255)
 gCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAAtgtg                                                                                                                                                     >  5:251782/1‑149 (MQ=255)
  cAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAAtgtg                                                                                                                                                     <  5:13864/148‑1 (MQ=255)
  cAGGGGAGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTgg                                                                                                                                                    >  4:53954/1‑149 (MQ=255)
      ggcggcCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCTGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCACATTCCAATGCGGccac                                                                                                                                                <  6:69824/149‑3 (MQ=255)
       gcggcCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCTGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCACATTCAAATGCGGccacc                                                                                                                                               <  6:200070/149‑4 (MQ=255)
           ccGGGGGGGGCGGCCTGTCAGCCGGTCAACCCGGCCGGGGTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGGGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGCAATGTGGCCGGCTGcc                                                                                                                                           <  8:207578/149‑1 (MQ=255)
             ggTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCgg                                                                                                                                         <  2:217251/149‑1 (MQ=255)
                  gggCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAAc                                                                                                                                    >  7:269368/1‑149 (MQ=255)
                    gCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAAc                                                                                                                                    >  8:280083/1‑147 (MQ=255)
                              aTCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGGTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATg                                                                                                                        >  1:233026/1‑149 (MQ=255)
                              aTCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATg                                                                                                                        >  7:266527/1‑149 (MQ=255)
                              aTCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCACATTCCAATGCGGccac                                                                                                                                                >  6:328370/1‑123 (MQ=255)
                              aTCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCACATTCCAATGCGGccac                                                                                                                                                <  5:328370/125‑3 (MQ=255)
                                  ggTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGt                                                                                                                    <  2:233026/149‑1 (MQ=255)
                                   gtaaaCCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCACATTCCAATGCGGcca                                                                                                                                                 <  5:222005/116‑2 (MQ=255)
                                   gtaaaCCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCACATTCCAATGCGGcca                                                                                                                                                 >  6:222005/4‑118 (MQ=255)
                                      aaCCCTGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGtttt                                                                                                                 <  6:294284/148‑1 (MQ=255)
                                      aaCCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTa                                                                                                                >  1:80115/1‑149 (MQ=255)
                                      aaCCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTa                                                                                                                >  7:10176/1‑149 (MQ=255)
                                                  ttCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGc                                                                                                    <  8:266527/149‑1 (MQ=255)
                                                         ttCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCAc                                                                                             >  7:140160/1‑149 (MQ=255)
                                                         ttCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCAc                                                                                             >  1:58908/1‑149 (MQ=255)
                                                             caGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCAt                                                                                                                         >  6:171337/1‑117 (MQ=255)
                                                             caGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCAt                                                                                                                         <  5:171337/117‑1 (MQ=255)
                                                                  ccATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGa                                                                                     <  8:269368/148‑1 (MQ=255)
                                                                  ccATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAg                                                                                    >  1:28811/1‑149 (MQ=255)
                                                                    aTTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAggg                                                                                  >  8:254129/1‑149 (MQ=255)
                                                                    aTTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAggg                                                                                  >  6:155459/1‑149 (MQ=255)
                                                                     ttCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGc                                                                                 >  8:222224/1‑149 (MQ=255)
                                                                        aCGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTc                                                                              >  1:146663/1‑149 (MQ=255)
                                                                              cccGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCgcgc                                                                          <  8:140160/147‑1 (MQ=255)
                                                                              cccGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTg                                                                        <  3:53954/149‑1 (MQ=255)
                                                                                   gAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACAt                                                                   <  2:58908/149‑1 (MQ=255)
                                                                                   gAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACAt                                                                   >  2:302948/1‑149 (MQ=255)
                                                                                           ccGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGaa                                                            >  2:276775/1‑148 (MQ=255)
                                                                                             ggTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAg                                                         <  6:251782/149‑1 (MQ=255)
                                                                                                gcgtgcTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCaa                                                      <  2:28811/149‑1 (MQ=255)
                                                                                                        cacacccacaccCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCt                                                                                         <  6:103073/106‑1 (MQ=255)
                                                                                                        cacacccacaccCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCt                                                                                         >  5:103073/1‑106 (MQ=255)
                                                                                                          cacccacacccGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCtggtgg                                            <  7:254129/149‑1 (MQ=255)
                                                                                                            cccacacccGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAAc                                                           <  4:132528/132‑1 (MQ=255)
                                                                                                            cccacacccGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAAc                                                           >  3:132528/1‑132 (MQ=255)
                                                                                                             ccacacccGCGCAGGCTGCGCGGTGGACCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGAt                                                               >  1:3727/1‑128 (MQ=255)
                                                                                                             ccacacccGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGAt                                                               <  2:3727/127‑1 (MQ=255)
                                                                                                             ccacacccGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGcc                                          >  1:121412/1‑148 (MQ=255)
                                                                                                             ccacacccGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGcc                                          >  1:116863/1‑148 (MQ=255)
                                                                                                             ccacacccGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGcc                                          >  4:173587/1‑148 (MQ=255)
                                                                                                             ccacacccGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCg                                         >  3:41236/1‑149 (MQ=255)
                                                                                                             acacacccGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGCGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGcc                                          >  6:271814/2‑148 (MQ=255)
                                                                                                                ccaccGCGCAGCCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGAcc                                      >  8:146730/4‑149 (MQ=255)
                                                                                                                    cgcgCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGa                                                                >  7:224052/1‑119 (MQ=255)
                                                                                                                    cgcgCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGa                                                                <  8:224052/119‑1 (MQ=255)
                                                                                                                    cgcgCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTggg                                  <  2:5073/149‑1 (MQ=255)
                                                                                                                     gcgcAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGc                                 <  7:280083/149‑1 (MQ=255)
                                                                                                                       gcAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGcaa                               <  2:80115/149‑1 (MQ=255)
                                                                                                                        cAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGcaac                              <  4:41236/149‑1 (MQ=255)
                                                                                                                           gCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACg                                                                                                              <  1:140288/66‑1 (MQ=255)
                                                                                                                           gCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAg                           >  7:89973/1‑149 (MQ=255)
                                                                                                                           gCTGAGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACg                                                                                                              >  2:140288/1‑66 (MQ=255)
                                                                                                                             tGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGcc                         >  8:210252/1‑149 (MQ=255)
                                                                                                                              gcgcGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGCCg                        <  8:10176/149‑1 (MQ=255)
                                                                                                                               cgcgGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGCCgg                       <  7:222224/149‑1 (MQ=255)
                                                                                                                               cgcgGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGCCgg                       <  6:291245/149‑1 (MQ=255)
                                                                                                                               cgcgGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGCCgg                       <  8:76419/149‑1 (MQ=255)
                                                                                                                                      g‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCTTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGCCGGtgatga                 <  7:210252/148‑1 (MQ=255)
                                                                                                                                         cGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGCCGGTGATGAtgc              >  5:321365/1‑149 (MQ=255)
                                                                                                                                          gCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAg                                                                                    >  6:190405/1‑78 (MQ=255)
                                                                                                                                                gAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGCCGGTGATGATGCTGCGCaa       >  8:294851/1‑149 (MQ=255)
                                                                                                                                                gAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGCCGGTGATGATGCTGCGCa        <  1:302948/148‑1 (MQ=255)
                                                                                                                                                     tgGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGCCGGTGATGATGCTGCGCAACCATg  >  8:2687/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGCAGGGGCGGCCGGTGGGGGCTGCCTGTCATCCGGTCAACCCGGCCGGGTTCGTCATTCACAGTGCCATTCACGCTGCCCGCGAGGATGCCCGGTGCGTGCTGCACACCCACACCCGCGCAGGCTGCGCGGTGG‑CCGCATTGGAATGTGGCCTGCTGCCGGTCAACCAGATGTCCATGGAGTTTTACGGCAATGTGGCTTACCACGCCTATGAGGGCGTCGCGCTGGACATGGATGAACAGCAACGGCTGGTGGCCGACCTGGGCAACAAGCCGGTGATGATGCTGCGCAACCATG  >  NC_002947/3273518‑3273814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑