Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,327,193 A→C 8.9% L338R (CTG→CGG)  udg ← UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,327,1930AC 8.9% 134.2 / 5.1 45L338R (CTG→CGG) udgUDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (20/21);  new base C (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

CCAGGGCATCGGCGCCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCGGCCCTTCTGCTGCTCGTT  >  NC_002947/3327047‑3327332
                                                                                                                                                  |                                                                                                                                           
ccAGGGCATCGGCGCCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCcagcag                                                                                                                                            >  7:157458/1‑148 (MQ=255)
 cAGGGCATCGGCGCCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGa                                                                                                                                          >  6:48682/1‑149 (MQ=255)
        tCGGCGCCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCt                                                                                                                                   >  1:184357/1‑149 (MQ=255)
         cGGCGCCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTg                                                                                                                                  >  3:205752/1‑149 (MQ=255)
            cgcCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCCGGGTCCTGCTGGaa                                                                                                                               <  6:115087/149‑1 (MQ=255)
            cgcCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGaa                                                                                                                               <  4:274485/149‑1 (MQ=255)
            cgcCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGGCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGaa                                                                                                                               <  7:89694/149‑1 (MQ=255)
                ccGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCtt                                                                                                                           >  3:224066/1‑149 (MQ=255)
                 cGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTc                                                                                                                          <  3:47807/149‑1 (MQ=255)
                   cAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTcgc                                                                                                                        <  5:48682/149‑1 (MQ=255)
                      cGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGa                                                                                                                     <  6:120444/149‑1 (MQ=255)
                       gCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGat                                                                                                                    <  4:224066/149‑1 (MQ=255)
                        cATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGata                                                                                                                   <  7:13415/149‑1 (MQ=255)
                         aTCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGata                                                                                                                   >  3:304445/1‑148 (MQ=255)
                            gTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCCGGGTCCTGCTGGAAg                                                                                                                              >  7:259440/1‑134 (MQ=255)
                            gTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCCGGGTCCTGCTGGAAg                                                                                                                              <  8:259440/134‑1 (MQ=255)
                             tCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCAtt                                                                                                              >  5:131956/1‑149 (MQ=255)
                               cTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTg                                                                                                             <  3:169994/148‑1 (MQ=255)
                                ttGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTgg                                                                                                            >  1:176727/1‑148 (MQ=255)
                                   cAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTAt                                                                                                         <  8:213493/148‑1 (MQ=255)
                                     gggCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTgg                                                                                                      <  8:157458/149‑1 (MQ=255)
                                         aCCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTg                                                                                                             <  7:115343/138‑1 (MQ=255)
                                         aCCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTg                                                                                                             >  8:115343/1‑138 (MQ=255)
                                         aCCAGTTGCAGGTCCGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCTCGCCCTCGGCCCACAGTCCTTCCAGCAGAACCCGGCTGGAAGCTTCGCGCATATCATAGGTATTGTGCtt                                                                                                  >  8:304350/1‑149 (MQ=255)
                                             gTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTgg                                                                                                            >  6:320961/1‑135 (MQ=255)
                                             gTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTgg                                                                                                            <  5:320961/135‑1 (MQ=255)
                                                  aGGTCGGCACGCGGGCCGTAGTGGGGCCGGGTTTCTTCCATGGCCTTAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGCCCCGGCTGGa                                                                                                                                >  7:47578/1‑110 (MQ=255)
                                                  aGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACaa                                                                                         >  6:101817/1‑149 (MQ=255)
                                                  aGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGACGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCACCAGGACCCGGCTGGa                                                                                                                                <  8:47578/110‑1 (MQ=255)
                                                            gcgGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAgg                                                                               <  4:205752/149‑1 (MQ=255)
                                                              gggCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGc                                                                             <  2:184357/149‑1 (MQ=255)
                                                                  cGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAac                                                                         <  7:18555/149‑1 (MQ=255)
                                                                       tGGCGTCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCGTGCACCCGCGCGCCAGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCtt                                                                    <  7:304350/149‑1 (MQ=255)
                                                                                     ttcCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCCGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGgccgc                                                      >  5:311592/1‑149 (MQ=255)
                                                                                            gCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGt                                                <  2:176727/148‑1 (MQ=255)
                                                                                                        tGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGAt                                   <  5:101817/149‑1 (MQ=255)
                                                                                                                 aCCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTATTCCCGCCGGCCGGCGGGGTAATGGCGCTGGATGTTGCTgca                          >  1:275067/1‑147 (MQ=255)
                                                                                                                   ccGCGCGCCCGCGGCCCGCAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAACCCCCTTGCCCCGCACGCCGCCGGGGTAATGGCGCTGGATCTTGCTGaa                          >  2:205306/1‑147 (MQ=255)
                                                                                                                   ccGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGaa                          >  2:178270/1‑147 (MQ=255)
                                                                                                                        ccactacgGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCgg                   <  5:129996/143‑1 (MQ=255)
                                                                                                                           ccGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTg                            >  3:72517/1‑137 (MQ=255)
                                                                                                                           ccGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTg                            <  4:72517/137‑1 (MQ=255)
                                                                                                                                  ccACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCGGCCCTTctgct         >  3:54673/1‑149 (MQ=255)
                                                                                                                                   cacaGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCGGCCCTTctgctg        >  2:2045/1‑149 (MQ=255)
                                                                                                                                    acaGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCGGCCCTTCtgctgc       >  6:6574/1‑149 (MQ=255)
                                                                                                                                         gCTTCCAGCCGGGTCCTGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCGGCCCTTCTGCTGCTCGtt  >  1:264440/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
CCAGGGCATCGGCGCCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCGGCCCTTCTGCTGCTCGTT  >  NC_002947/3327047‑3327332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑