Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,327,196 A→G 9.3% V337A (GTC→GCC) ‡ udg ← UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,327,1960AG 9.3% 109.3 / 2.9 44V337A (GTC→GCC) ‡udgUDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (18/21);  new base G (2/2);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

CAGGGCATCGGCGCCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCGGCCCTTCTGCTGCTCGTT  >  NC_002947/3327048‑3327332
                                                                                                                                                    |                                                                                                                                        
cAGGGCATCGGCGCCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGa                                                                                                                                          >  6:48682/1‑149 (MQ=255)
       tCGGCGCCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCt                                                                                                                                   >  1:184357/1‑149 (MQ=255)
        cGGCGCCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTg                                                                                                                                  >  3:205752/1‑149 (MQ=255)
           cgcCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCCGGGTCCTGCTGGaa                                                                                                                               <  6:115087/149‑1 (MQ=255)
           cgcCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGaa                                                                                                                               <  4:274485/149‑1 (MQ=255)
           cgcCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGGCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGaa                                                                                                                               <  7:89694/149‑1 (MQ=255)
               ccGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCtt                                                                                                                           >  3:224066/1‑149 (MQ=255)
                cGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTc                                                                                                                          <  3:47807/149‑1 (MQ=255)
                  cAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTcgc                                                                                                                        <  5:48682/149‑1 (MQ=255)
                     cGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGa                                                                                                                     <  6:120444/149‑1 (MQ=255)
                      gCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGat                                                                                                                    <  4:224066/149‑1 (MQ=255)
                       cATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGata                                                                                                                   <  7:13415/149‑1 (MQ=255)
                        aTCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGata                                                                                                                   >  3:304445/1‑148 (MQ=255)
                           gTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCCGGGTCCTGCTGGAAg                                                                                                                              <  8:259440/134‑1 (MQ=255)
                           gTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCCGGGTCCTGCTGGAAg                                                                                                                              >  7:259440/1‑134 (MQ=255)
                            tCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCAtt                                                                                                              >  5:131956/1‑149 (MQ=255)
                              cTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTg                                                                                                             <  3:169994/148‑1 (MQ=255)
                               ttGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTgg                                                                                                            >  1:176727/1‑148 (MQ=255)
                                  cAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTAt                                                                                                         <  8:213493/148‑1 (MQ=255)
                                    gggCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTgg                                                                                                      <  8:157458/149‑1 (MQ=255)
                                        aCCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTg                                                                                                             >  8:115343/1‑138 (MQ=255)
                                        aCCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTg                                                                                                             <  7:115343/138‑1 (MQ=255)
                                        aCCAGTTGCAGGTCCGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCTCGCCCTCGGCCCACAGTCCTTCCAGCAGAACCCGGCTGGAAGCTTCGCGCATATCATAGGTATTGTGCtt                                                                                                  >  8:304350/1‑149 (MQ=255)
                                            gTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTgg                                                                                                            <  5:320961/135‑1 (MQ=255)
                                            gTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTgg                                                                                                            >  6:320961/1‑135 (MQ=255)
                                                 aGGTCGGCACGCGGGCCGTAGTGGGGCCGGGTTTCTTCCATGGCCTTAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGCCCCGGCTGGa                                                                                                                                >  7:47578/1‑110 (MQ=255)
                                                 aGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACaa                                                                                         >  6:101817/1‑149 (MQ=255)
                                                 aGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGACGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCACCAGGACCCGGCTGGa                                                                                                                                <  8:47578/110‑1 (MQ=255)
                                                           gcgGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAgg                                                                               <  4:205752/149‑1 (MQ=255)
                                                             gggCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGc                                                                             <  2:184357/149‑1 (MQ=255)
                                                                 cGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAac                                                                         <  7:18555/149‑1 (MQ=255)
                                                                      tGGCGTCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCGTGCACCCGCGCGCCAGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCtt                                                                    <  7:304350/149‑1 (MQ=255)
                                                                                    ttcCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCCGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGgccgc                                                      >  5:311592/1‑149 (MQ=255)
                                                                                           gCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGt                                                <  2:176727/148‑1 (MQ=255)
                                                                                                       tGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGAt                                   <  5:101817/149‑1 (MQ=255)
                                                                                                                aCCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTATTCCCGCCGGCCGGCGGGGTAATGGCGCTGGATGTTGCTgca                          >  1:275067/1‑147 (MQ=255)
                                                                                                                  ccGCGCGCCCGCGGCCCGCAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAACCCCCTTGCCCCGCACGCCGCCGGGGTAATGGCGCTGGATCTTGCTGaa                          >  2:205306/1‑147 (MQ=255)
                                                                                                                  ccGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGaa                          >  2:178270/1‑147 (MQ=255)
                                                                                                                       ccactacgGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCgg                   <  5:129996/143‑1 (MQ=255)
                                                                                                                          ccGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTg                            <  4:72517/137‑1 (MQ=255)
                                                                                                                          ccGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTg                            >  3:72517/1‑137 (MQ=255)
                                                                                                                                 ccACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCGGCCCTTctgct         >  3:54673/1‑149 (MQ=255)
                                                                                                                                  cacaGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCGGCCCTTctgctg        >  2:2045/1‑149 (MQ=255)
                                                                                                                                   acaGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCGGCCCTTCtgctgc       >  6:6574/1‑149 (MQ=255)
                                                                                                                                        gCTTCCAGCCGGGTCCTGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCGGCCCTTCTGCTGCTCGtt  >  1:264440/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                        
CAGGGCATCGGCGCCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCGGCCCTTCTGCTGCTCGTT  >  NC_002947/3327048‑3327332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑