Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,327,200 G→T 8.9% R336R (CGG→AGG)  udg ← UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,327,2000GT 8.9% 130.3 / 4.2 45R336R (CGG→AGG) udgUDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (19/21);  new base T (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.12e-01

TCGGCGCCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCGGCCCTTCTGCTGCTCGTTGACCGACTCTACTG  >  NC_002947/3327055‑3327346
                                                                                                                                                 |                                                                                                                                                  
tCGGCGCCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCt                                                                                                                                                 >  1:184357/1‑149 (MQ=255)
 cGGCGCCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTg                                                                                                                                                >  3:205752/1‑149 (MQ=255)
    cgcCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCCGGGTCCTGCTGGaa                                                                                                                                             <  6:115087/149‑1 (MQ=255)
    cgcCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGaa                                                                                                                                             <  4:274485/149‑1 (MQ=255)
    cgcCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGGCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGaa                                                                                                                                             <  7:89694/149‑1 (MQ=255)
        ccGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCtt                                                                                                                                         >  3:224066/1‑149 (MQ=255)
         cGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTc                                                                                                                                        <  3:47807/149‑1 (MQ=255)
           cAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTcgc                                                                                                                                      <  5:48682/149‑1 (MQ=255)
              cGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGa                                                                                                                                   <  6:120444/149‑1 (MQ=255)
               gCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGat                                                                                                                                  <  4:224066/149‑1 (MQ=255)
                cATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGata                                                                                                                                 <  7:13415/149‑1 (MQ=255)
                 aTCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGata                                                                                                                                 >  3:304445/1‑148 (MQ=255)
                    gTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCCGGGTCCTGCTGGAAg                                                                                                                                            >  7:259440/1‑134 (MQ=255)
                    gTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCCGGGTCCTGCTGGAAg                                                                                                                                            <  8:259440/134‑1 (MQ=255)
                     tCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCAtt                                                                                                                            >  5:131956/1‑149 (MQ=255)
                       cTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTg                                                                                                                           <  3:169994/148‑1 (MQ=255)
                        ttGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTgg                                                                                                                          >  1:176727/1‑148 (MQ=255)
                           cAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTAt                                                                                                                       <  8:213493/148‑1 (MQ=255)
                             gggCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTgg                                                                                                                    <  8:157458/149‑1 (MQ=255)
                                 aCCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTg                                                                                                                           >  8:115343/1‑138 (MQ=255)
                                 aCCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTg                                                                                                                           <  7:115343/138‑1 (MQ=255)
                                 aCCAGTTGCAGGTCCGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCTCGCCCTCGGCCCACAGTCCTTCCAGCAGAACCCGGCTGGAAGCTTCGCGCATATCATAGGTATTGTGCtt                                                                                                                >  8:304350/1‑149 (MQ=255)
                                     gTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTgg                                                                                                                          >  6:320961/1‑135 (MQ=255)
                                     gTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTgg                                                                                                                          <  5:320961/135‑1 (MQ=255)
                                          aGGTCGGCACGCGGGCCGTAGTGGGGCCGGGTTTCTTCCATGGCCTTAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGCCCCGGCTGGa                                                                                                                                              >  7:47578/1‑110 (MQ=255)
                                          aGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACaa                                                                                                       >  6:101817/1‑149 (MQ=255)
                                          aGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGACGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCACCAGGACCCGGCTGGa                                                                                                                                              <  8:47578/110‑1 (MQ=255)
                                                    gcgGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAgg                                                                                             <  4:205752/149‑1 (MQ=255)
                                                      gggCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGc                                                                                           <  2:184357/149‑1 (MQ=255)
                                                          cGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAac                                                                                       <  7:18555/149‑1 (MQ=255)
                                                               tGGCGTCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCGTGCACCCGCGCGCCAGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCtt                                                                                  <  7:304350/149‑1 (MQ=255)
                                                                             ttcCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCCGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGgccgc                                                                    >  5:311592/1‑149 (MQ=255)
                                                                                    gCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGt                                                              <  2:176727/148‑1 (MQ=255)
                                                                                                tGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGAt                                                 <  5:101817/149‑1 (MQ=255)
                                                                                                         aCCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTATTCCCGCCGGCCGGCGGGGTAATGGCGCTGGATGTTGCTgca                                        >  1:275067/1‑147 (MQ=255)
                                                                                                           ccGCGCGCCCGCGGCCCGCAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAACCCCCTTGCCCCGCACGCCGCCGGGGTAATGGCGCTGGATCTTGCTGaa                                        >  2:205306/1‑147 (MQ=255)
                                                                                                           ccGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGaa                                        >  2:178270/1‑147 (MQ=255)
                                                                                                                ccactacgGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCgg                                 <  5:129996/143‑1 (MQ=255)
                                                                                                                   ccGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTg                                          >  3:72517/1‑137 (MQ=255)
                                                                                                                   ccGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTg                                          <  4:72517/137‑1 (MQ=255)
                                                                                                                          ccACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCGGCCCTTctgct                       >  3:54673/1‑149 (MQ=255)
                                                                                                                           cacaGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCGGCCCTTctgctg                      >  2:2045/1‑149 (MQ=255)
                                                                                                                            acaGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCGGCCCTTCtgctgc                     >  6:6574/1‑149 (MQ=255)
                                                                                                                                 gCTTCCAGCCGGGTCCTGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCGGCCCTTCTGCTGCTCGtt                >  1:264440/1‑149 (MQ=255)
                                                                                                                                               ccGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCGGCCCTTCTGCTGCTCGTTGACCGACTCTACTg  >  1:127507/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TCGGCGCCCCGCAACGCATCGTCCTTGCAGGGCACCAGTTGCAGGTCGGCACGCGGGCCGTAGTGGCGCCGGATTTCTTCCATGGCCTGAGGGTCGTGAGCCTGCACCCGCGCGCCCGCGGCCCACAGTGCTTCCAGCAGGACCCGGCTGGAAGCTTCGCGGATATCATTGGTATTGGGCTTGAACGACAACCCCCACAGGGCAAACACCTTGCCCCGCAGGCCGCCGGGGTAATGGCGCTGGATCTTGCTGAACAGCCGGCCCTTCTGCTGCTCGTTGACCGACTCTACTG  >  NC_002947/3327055‑3327346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑