Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,511,916 A→C 8.0% K195T (AAA→ACA) ‡ PP_3108 → rhs family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,511,9160AC 8.0% 149.3 / 4.6 50K195T (AAA→ACA) ‡PP_3108rhs family protein
Reads supporting (aligned to +/- strand):  ref base A (24/22);  new base C (2/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

TCATCGGCGGCGGCACCTACCGTTACCTGCCCGTGGACGATGAAGTCCCGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAACCCGTGGCCCGCAAGGCTATCGGCGGCCTGGTCGGCAACCCCGTCGACCTCACGACGGGCCGCAAGCTGATC  >  NC_002947/3511769‑3512064
                                                                                                                                                   |                                                                                                                                                    
tcatcGGCGGCGGCACCTACCGTTACCTGCCCGTGGACGATGAAGTCCCGGAGTGGCGGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGaaa                                                                                                                                                     <  8:127206/149‑1 (MQ=255)
             cACCTACCGTTACCTGCCCGTGGACGATGAAGTCCCGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCtt                                                                                                                                        <  3:87149/149‑1 (MQ=255)
                cTACCGTTACCTGCCCGTGGACGATGAAGTCCCGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTA‑CCAGGCCGGCATGACGGCTTTCATGccg                                                                                                                                         <  1:257542/144‑2 (MQ=255)
                cTACCGTTACCTGCCCGTGGACGATGAAGTCCCGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTA‑CCAGGCCGGCATGACGGCTTTCATGccg                                                                                                                                         >  2:257542/1‑143 (MQ=255)
                 aaCCGTTACCTGCCCGTGGACGATGAAGTCCCGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTgcgc                                                                                                                                    <  1:282318/148‑1 (MQ=255)
                    cGTTACCTGCCCGTGGACGATGAAGTCCCGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCt                                                                                                                                 >  8:268323/1‑149 (MQ=255)
                    cGTTACCTGCCCGTGGACGATGAAGTCCCGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCt                                                                                                                                 >  4:172955/1‑149 (MQ=255)
                    cGTTACCTGCCCGTGGACGATGAAGTCCCGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCt                                                                                                                                 >  2:142540/1‑149 (MQ=255)
                     gTTACCTGCCCGTGGACGATGAAGTCCCGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTg                                                                                                                                <  4:308592/149‑1 (MQ=255)
                                   gACGATGAAGTCCCGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCAcggccgg                                                                                                                  >  3:276323/1‑149 (MQ=255)
                                      gATGAAGTCCCGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGACGGCTTTCATGccggcc                                                                                                                                      <  7:173751/126‑5 (MQ=255)
                                      gATGAAGTCCCGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGACGGCTTTCATGccggcc                                                                                                                                      >  8:173751/1‑122 (MQ=255)
                                      gATGAAGTCCCGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCtt                                                                                                               >  2:276673/1‑149 (MQ=255)
                                        tGAAGTCCCGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGaaa                                                                                                                                                     <  4:161757/109‑1 (MQ=255)
                                        tGAAGTCCCGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGaaa                                                                                                                                                     >  3:161757/1‑109 (MQ=255)
                                        tGAAGTCCCGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTtcg                                                                                                             >  6:158613/1‑149 (MQ=255)
                                           aGTCCCGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTtcgtcg                                                                                                          >  5:119420/1‑149 (MQ=255)
                                             tCCCGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGcc                                                                                                        >  4:225912/1‑149 (MQ=255)
                                               ccGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCg                                                                                                       <  3:172955/148‑1 (MQ=255)
                                                cGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGt                                                                                                     >  8:15139/1‑149 (MQ=255)
                                                     tGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCACGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGt                                                                                                <  7:208668/149‑1 (MQ=255)
                                                               ccaccGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCAt                                                                                                                                             <  5:124182/94‑1 (MQ=255)
                                                               ccaccGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGc                                                                                                         >  4:146493/1‑130 (MQ=255)
                                                               ccaccGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGc                                                                                                         <  3:146493/130‑1 (MQ=255)
                                                               ccaccGTGGATGTGCTGATGGCCATCGCCGGTGACGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCAt                                                                                                                                             >  6:124182/1‑94 (MQ=255)
                                                                caccGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGt                                                                                      >  4:193457/1‑148 (MQ=255)
                                                                   cGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTAtg                                                                                  <  3:193457/149‑1 (MQ=255)
                                                                   cGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTAtg                                                                                  <  7:268323/149‑1 (MQ=255)
                                                                   cGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTAtg                                                                                  <  1:142540/149‑1 (MQ=255)
                                                                    gTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTAtgt                                                                                 <  4:276323/149‑1 (MQ=255)
                                                                       gATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCa                                                                                           >  2:186147/1‑136 (MQ=255)
                                                                       gATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACCGCCGGCTTCGTCGCCGGTGAGGTGGCCa                                                                                           <  1:186147/136‑1 (MQ=255)
                                                                             cTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAAcc                                                                        >  6:229760/1‑149 (MQ=255)
                                                                             cTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAAc                                                                         <  5:158613/148‑1 (MQ=255)
                                                                             cTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAAc                                                                         <  6:28046/148‑1 (MQ=255)
                                                                              tGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAAccc                                                                       >  8:108738/1‑149 (MQ=255)
                                                                                aTGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAACCCGt                                                                     <  5:157317/149‑1 (MQ=255)
                                                                                          ccGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAACCCGTGGCCCGCAAg                                                           <  1:276673/149‑1 (MQ=255)
                                                                                             gTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGa                                                                                                   >  7:202167/1‑106 (MQ=255)
                                                                                                  gCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAACCCGTGGCCCGCAAGGCTATcgg                                                   >  3:169788/1‑149 (MQ=255)
                                                                                                           aTCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGt                                                                              >  4:302635/1‑113 (MQ=255)
                                                                                                           aTCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGt                                                                              <  3:302635/113‑1 (MQ=255)
                                                                                                            tCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAACCCGTGGCCCGCAAGGCTATCGGCGGCCTGGTc                                         <  5:184306/149‑1 (MQ=255)
                                                                                                                  aGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAACCCGTGGCCCGCAAGGCTATCGGCGGCCTGGTCGGCAAc                                   <  7:108738/149‑1 (MQ=255)
                                                                                                                       aTCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAACCCGTGGCCCGCAAGGCTATCGGCGGCCTGGTCGGCAACCCCGt                              >  1:241876/1‑149 (MQ=255)
                                                                                                                        tCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAACCCGTGGCCCGCAAGGCTATCGGCGGCCTGGTCGGCAACCCCGTc                             >  2:315932/1‑149 (MQ=255)
                                                                                                                           aGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAACCCGTGGCCCGCAAGGCTATCGGCGGCCTGGTCGGCAACCCCGTCGAc                          <  7:15139/149‑1 (MQ=255)
                                                                                                                                 gTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAACCCGTGGCCCGCAAGGCTATCGGCGGCCTGGTCGGCAACCCCGTCGACCTCacg                    >  7:298447/1‑149 (MQ=255)
                                                                                                                                       aGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAACCCGTGGCCCGCAAGGCTATCGGCGGCCTGGTCGGCAACCCCGTCGACCTCACGACGGGc              >  8:7615/1‑149 (MQ=255)
                                                                                                                                            ggCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAACCCGTGGCCCGCAAGGCTATCGGCGGCCTGGTCGGCAACCCCGTCGACCTCACGACGGGCCGCaa         <  8:298447/149‑1 (MQ=255)
                                                                                                                                             gCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAACCCGTGGCCCGCAAGGCTATCGGCGGCCTGGTCGGCAACCCCGTCGACCTCACGACgg                >  6:85199/1‑141 (MQ=255)
                                                                                                                                             gCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAACCCGTGGCCCGCAAGGCTATCGGCGGCCTGGTCGGCAACCCCGTCGACCTCACGACgg                <  5:85199/141‑1 (MQ=255)
                                                                                                                                                tGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAACCCGTGGCCCGCAAGGCTATCGGCGGCCTGGTCGGCAACCCCGTCGACCTCACGACGGGCCGCAAGCt      >  3:247091/1‑148 (MQ=255)
                                                                                                                                                   aaGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAACCCGTGGCCCGCAAGGCTATCGGCGGCCTGGTCGGCAACCCCGTCGACCTCACGACGGGCCGCAAGCTGATc  >  6:46244/1‑149 (MQ=255)
                                                                                                                                                   aaGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAACCCGTGGCCCGCAAGGCTATCGGCGGCCTGGTCGGCAACCCCGTCGACCTCACGACGGGCCGCAAGCTGATc  <  1:315932/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TCATCGGCGGCGGCACCTACCGTTACCTGCCCGTGGACGATGAAGTCCCGGAGTGGCTGCGCACCACCGTGGATGTGCTGATGGCCATCGCCGGTGCCGCAGGCGGTATCGCCCAGCTGATCAAGGCGGGTACCCAGGCCGGCATGAAAGCCGTCATGCCTTGCGCCCTGAAGTTCACGGCCGGCTTCGTCGCCGGTGAGGTGGCCAGCCGTTATGTGGTCGAACCCGTGGCCCGCAAGGCTATCGGCGGCCTGGTCGGCAACCCCGTCGACCTCACGACGGGCCGCAAGCTGATC  >  NC_002947/3511769‑3512064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑