Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 353,804 C→G 6.3% V176L (GTG→CTG) ‡ cbcV ← choline/betaine/carnitine ABC transporter ATP binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947353,8040CG 6.3% 202.6 / 3.9 63V176L (GTG→CTG) ‡cbcVcholine/betaine/carnitine ABC transporter ATP binding protein
Reads supporting (aligned to +/- strand):  ref base C (29/30);  new base G (2/2);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

ACACGATGGTTTTGCTCAGCTTGGCCTGCAGGCCAAGCAGCTCATCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGCCGTTCCTTTTCCGGGCGACCCTGCATTTCCAGGCCGAAGCTGATGTTCTCGC  >  NC_002947/353663‑353950
                                                                                                                                             |                                                                                                                                                  
acacGATGGTTTTGCTCAGCTTGGCCTGCAGGCCAAGCAGCTCATCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCgctgc                                                                                                                                             >  1:291438/1‑149 (MQ=255)
 cacGATGGTGTTGCTCAGCTTGGCCTGCAGGCCAAGCAGCTCATCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCgctgct                                                                                                                                            <  4:57615/149‑1 (MQ=255)
    gATGGTTTTGCTCAGCTTGGCCTGCAGGCCAAGCAGCTCATCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCa                                                                                                                                         >  8:250800/1‑149 (MQ=255)
         ttttGCTCAGCTTGGCCTGCAGGCCAAGCAGCTCATCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATgccg                                                                                                                                    >  1:129851/1‑149 (MQ=255)
               tCAGCTTGGCCTGCAGGCCAAGCAGCTCATCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCGTGGGCCTGGCCCGGGCgctggcga                                                                                                                                          <  8:211636/137‑5 (MQ=255)
               tCAGCTTGGCCTGCAGGCCAAGCAGCTCATCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCGTGGGCCTGGCCCGGGCgctggcga                                                                                                                                          >  7:211636/1‑133 (MQ=255)
               tCAGCTTGGCCTGCAGGCCAAGCAGCTCATCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGa                                                                                                                               >  5:266968/1‑148 (MQ=255)
               tCAGCTTGGCCTGCAGGCCAAGCAGCTCATCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGa                                                                                                                               >  3:258872/1‑148 (MQ=255)
                 aGCTTGGCCTGCAGGCCAAGCAGCTCATCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGaaa                                                                                                                             <  2:291438/148‑1 (MQ=255)
                          tGCAGGCCAAGCAGCTCATCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATgccgc                                                                                                                                   <  4:320551/133‑1 (MQ=255)
                          tGCAGGCCAAGCAGCTCATCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATgccgc                                                                                                                                   >  3:320551/1‑133 (MQ=255)
                           gCAGGCCAAGCAGCTCATCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCtgctgc                                                                                                                                          <  6:185333/125‑1 (MQ=255)
                           gCAGGCCAAGCAGCTCATCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCtgctgc                                                                                                                                          >  5:185333/1‑125 (MQ=255)
                              ggCCAAGCAGCTCTTCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGcttc                                                                                                               <  6:272989/149‑1 (MQ=255)
                               gCCAAGCAGCTCATCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGcttct                                                                                                              >  6:154926/1‑149 (MQ=255)
                                ccAAGCAGCTCATCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGcttctt                                                                                                             <  1:65828/149‑1 (MQ=255)
                                  aaGCAGCTCATCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCttgt                                                                                                           >  2:127583/1‑149 (MQ=255)
                                   agcagcTCATCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCttgtt                                                                                                          <  4:258872/149‑1 (MQ=255)
                                         tCATCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCGGGATGTCGGCATCCATCGCCAGCGCCCGGGCCCGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGccc                                                                                                    >  2:278767/1‑148 (MQ=255)
                                           aTCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGcttc                                                                                                               <  7:216568/136‑1 (MQ=255)
                                           aTCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGcttc                                                                                                               >  8:216568/1‑136 (MQ=255)
                                              ttGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTggg                                                                                               >  5:161688/1‑149 (MQ=255)
                                                gCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCAc                                                                                                   <  8:12595/143‑1 (MQ=255)
                                                gCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCAc                                                                                                   >  7:12595/1‑143 (MQ=255)
                                                gCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTgg                                                                                                <  7:250800/146‑1 (MQ=255)
                                                gCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTgg                                                                                                <  8:40910/146‑1 (MQ=255)
                                                  aGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAg                                                                                           >  5:261040/1‑149 (MQ=255)
                                                      ccTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCtgctgc                                                                                                                                          <  6:33908/98‑1 (MQ=255)
                                                      ccTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCtgctgc                                                                                                                                          >  5:33908/1‑98 (MQ=255)
                                                       cTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATc                                                                                                                      <  2:10938/117‑1 (MQ=255)
                                                       cTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATc                                                                                                                      >  1:10938/1‑117 (MQ=255)
                                                                 aGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATgccg                                                                                                                                    <  6:5432/93‑1 (MQ=255)
                                                                 aGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATgccg                                                                                                                                    >  5:5432/1‑93 (MQ=255)
                                                                  gTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCAGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGc                                                                           >  2:73423/1‑149 (MQ=255)
                                                                     gATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTtc                                                                        <  6:161688/149‑1 (MQ=255)
                                                                         cAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCtcgt                                                                    <  8:31735/149‑1 (MQ=255)
                                                                            ggCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGa                                                                 >  4:215601/1‑149 (MQ=255)
                                                                               cGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCa                                                              >  6:133086/1‑149 (MQ=255)
                                                                                        ttCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGcc                                                     >  1:177466/1‑149 (MQ=255)
                                                                                           gTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGCCGtt                                                  <  5:154926/149‑1 (MQ=255)
                                                                                            tCCATCAGCAGGATGTCGGCATCCATCTCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGCCGTTc                                                 <  6:265309/149‑1 (MQ=255)
                                                                                            tCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCccac                                                                                     >  1:10946/1‑113 (MQ=255)
                                                                                            tCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCccac                                                                                     <  2:10946/113‑1 (MQ=255)
                                                                                              cATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGCCGTTcc                                                <  6:266968/148‑1 (MQ=255)
                                                                                              cATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGCCGTTcc                                                <  6:261040/148‑1 (MQ=255)
                                                                                                tCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGCCGTTCCttt                                             >  8:67907/1‑149 (MQ=255)
                                                                                                  agcagGAGGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGCCGTTCCtttt                                            <  6:56293/148‑1 (MQ=255)
                                                                                                   gcagGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGCCGTTCCTTTTcc                                          >  2:264405/1‑149 (MQ=255)
                                                                                                   gcagGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGCCGTTCCTTTTcc                                          >  8:195058/1‑149 (MQ=255)
                                                                                                       gATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGCCGTTCCTTTTCCGGGc                                      <  7:195058/149‑1 (MQ=255)
                                                                                                             ggCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGCCGTTCCTTTTCCGGGCGACCCt                                <  2:177466/149‑1 (MQ=255)
                                                                                                                 tccatcGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGCCGTTCCTTTTCCGGGCGACCCTGCAt                            <  1:264405/149‑1 (MQ=255)
                                                                                                                   catcGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGCCGTTCCTTTTCCGGGCGACCCTGCAttt                          >  2:170370/1‑149 (MQ=255)
                                                                                                                      cagcAGCGCGTGGGCCTGGCCCGGGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCtcg                                                                     >  6:263772/4‑103 (MQ=38)
                                                                                                                      cagcAGCGCGTGGGCCTGGCCCGGGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCtcg                                                                     <  5:263772/100‑1 (MQ=38)
                                                                                                                       gCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGCCGTTCCTTTTCCGGGCGACCCTGCATTTCCAg                      >  1:125995/1‑149 (MQ=255)
                                                                                                                        ccAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGCCGTTCCTTTTCCGGGCGACCCTGCATTTCCAgg                     <  2:125995/149‑1 (MQ=255)
                                                                                                                          aGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGc                                                            >  8:218450/1‑108 (MQ=255)
                                                                                                                          aGCGCCCGGGACAGGCCCACGCGCTTCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGc                                                            <  7:218450/108‑1 (MQ=255)
                                                                                                                             gcCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGCCGTTCCTTTTCCGGGCGACCCTGCATTTCCAGGCCGaa                >  8:192137/1‑149 (MQ=255)
                                                                                                                                   gCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGCCGTTCCTTTTCCGGGCGACCCTGCATTTCCAGGCCGAAGCTGAt          <  1:170370/149‑1 (MQ=255)
                                                                                                                                       ggCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGCCGTTCCTTTTCCGGGCGACCCTGCATTTCCAGGCCGAAGCTGATGTtc      <  7:67907/149‑1 (MQ=255)
                                                                                                                                           cACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGCCGTTCCTTTTCCGGGCGACCCTGCATTTCCAGGCCGAAGCTGATGTTCTcgc  <  1:127583/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
ACACGATGGTTTTGCTCAGCTTGGCCTGCAGGCCAAGCAGCTCATCTTGCAGGCCCTGGCGGATCAGTGGATCCAGGGCCGAGAACGGTTCGTCCATCAGCAGGATGTCGGCATCCATCGCCAGCGCCCGGGCCAGGCCCACGCGCTGCTGCATGCCGCCAGAAAGCTCATCCGGCTTCTTGTTGCGCCACTGGGTCAGGCCCACCAGCTCAAGCTTCTCGTCGACCAGCTTGCGCCGTTCCTTTTCCGGGCGACCCTGCATTTCCAGGCCGAAGCTGATGTTCTCGC  >  NC_002947/353663‑353950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑