Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,535,669 T→C 10.5% W268R (TGG→CGG)  PP_3124 → short chain fatty acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,535,6690TC10.5% 96.4 / 2.9 38W268R (TGG→CGG) PP_3124short chain fatty acid transporter
Reads supporting (aligned to +/- strand):  ref base T (15/19);  new base C (2/2);  total (17/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

CGGGCCCGAACAGCGCCCGTAGCGCAGAAGACTGCGGTGTCGACCCCAGCTTCACAGCGCCACCCGCGCCGCAACGCACGCGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCTGTTGTTCATCATGCTCGGTGCTTTGCTGCACTGGCGCCCACGCAGCTTCCTTGACGCGGTGGCGCGTGCGGTACCGAC  >  NC_002947/3535523‑3535814
                                                                                                                                                  |                                                                                                                                                 
cGGGCCCGAACAGCGCCCGTAGCGCAGAAGACTGCGGTGTCGACCCCAGCTTCACAGCGCCACCCGCGCCGCAACGCACGCGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTgctggctgg                                                                                                                                                 <  1:219191/149‑1 (MQ=255)
  ggCCCGAACAGCGCCCGTAGCGCAGAAGACTGCGGTGTCGACCCCAGCTTCACAGCGCCACCCGCGCCGCAACGCACGCGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTgctggctggct                                                                                                                                               <  7:72933/149‑1 (MQ=255)
   gCCCGAACAGCGCCCGTAGCGCAGAAGACTGCGGTGTCGACCCCAGCTTCACAGCGCCACCCGCGCCGCAACGCACGCGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTgctggctggctg                                                                                                                                              <  5:237315/149‑1 (MQ=255)
        aaCAGCGCCCGTAGCGCAGAAGACTGCGGTGTCGACCCCAGCTTCACAGCGCCACCCGCGCCGCAACGCACGCGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCa                                                                                                                                         <  8:57549/149‑1 (MQ=255)
               cccGTAGCGCAGAAGACTGCGGTGTCGACCCCAGCTTCACAGCGCCACCCGCGCCGCAACGCACGCGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGttt                                                                                                                                  <  8:259139/149‑1 (MQ=255)
                   tatcgcAGAAGACTGCGGTGTCGACCCCAGCTTCACAGCGCCACCCGCGCCGCAACGCACGCGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGcga                                                                                                                              <  4:140312/146‑1 (MQ=255)
                       gcAGAAGACTGCGGTGTCGACCCCAGCTTCACAGCGCCACCCGCGCCGCAACGCACGCGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTCGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGcgacga                                                                                                                           >  5:184369/1‑148 (MQ=255)
                                    gtgtCGACCCCAGCTTCACAGCGCCACCCGCGCCGCAACGCACGCGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTAcc                                                                                                             <  6:247907/149‑1 (MQ=255)
                                         gACCCCAGCTTCACAGCGCCACCCGCGCCGCAACGCACGCGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTT‑GTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCAt                                                                                                        <  2:207064/148‑1 (MQ=255)
                                             ccAGCTTCACAGCGCCACCCGCGCCGCAACGCACGCGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCg                                                                                                    >  3:147238/1‑149 (MQ=255)
                                             ccAGCTTCACAGCGCCACCCGCGCCGCAACGCACGCGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCg                                                                                                    <  3:257630/149‑1 (MQ=255)
                                             ccAGCTTCACAGCGCCACCCGCGCCGCAACGCACGCGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGACCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCg                                                                                                    >  5:256717/1‑149 (MQ=255)
                                              cAGCTTCACAGCGCCACCCGCGCCGCAACGCACGCGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCgg                                                                                                   <  4:150107/149‑1 (MQ=255)
                                               aGCTTCACAGCGCCACCCGCGCCGCAACGCACGCGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCggg                                                                                                  <  7:13245/149‑1 (MQ=255)
                                                     acaGCGCCACCCGCGCCGCAACGCACGCGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCaa                                                                                            <  1:296202/149‑1 (MQ=255)
                                                             aCCCGCGCCGCAACGCACGCGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTAc                                                                                     >  4:102931/1‑148 (MQ=255)
                                                                cgcgCCGCAACGCACGCGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAAcc                                                                                 >  3:244703/1‑149 (MQ=255)
                                                                      gcAACGCACGCGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCAACTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCtgttgt                                                                           >  1:247081/1‑149 (MQ=255)
                                                                          cgcacgcGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCCGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCTGTTGTtcat                                                                       >  8:58920/1‑149 (MQ=255)
                                                                          cgcacgcGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCCGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCTGTTGTtca                                                                        >  8:54543/1‑148 (MQ=255)
                                                                           gcacgcGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGACCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCTGTTGTtcatc                                                                      <  6:256717/149‑1 (MQ=255)
                                                                                      ggAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCTGTTGTTCATCATGCTCGGTGc                                                           <  6:127470/149‑1 (MQ=255)
                                                                                       gagaGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCtgtt                                                                             <  4:43406/130‑1 (MQ=255)
                                                                                       gagaGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCtgtt                                                                             >  3:43406/1‑130 (MQ=255)
                                                                                                tGGAACACAGACCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGGCATCTCGGGGCTCAACACCTACAACCTGTTGTTCATCATGCTCGGTGCTTTGCTGCAc                                                 >  6:241254/1‑149 (MQ=255)
                                                                                                 ggAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCggg                                                                                                  <  3:246340/99‑1 (MQ=255)
                                                                                                 ggAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCggg                                                                                                  >  4:246340/1‑99 (MQ=255)
                                                                                                             gATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCTGTTGTTCATCATGCTCGGTGCTTTGCTGCACTGGCGCCCACGCa                                    <  3:102931/149‑1 (MQ=255)
                                                                                                             gATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCTGTTGTTCATCATGCTCGGTGCTTTGCTGCACTGGCGCACACg                                      >  1:136604/1‑147 (MQ=255)
                                                                                                                  ttATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCgg                                                                                                   >  6:122282/1‑81 (MQ=255)
                                                                                                                  ttATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCgg                                                                                                   <  5:122282/81‑1 (MQ=255)
                                                                                                                         gctgTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCTGTTGTTCATCATGCTCGGTGCTTTGCTGCACTGGCGCCCACGCAGCTTCCTTGAcg                        >  2:164349/1‑149 (MQ=255)
                                                                                                                                gtggCCTTGGCTGCTGGCCGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCTGTTGTTCATCATGCTCGGTGCTTTGCTGCACTGGCGCCCACGCAGCTTCCTTGACGCGGTGgc                 <  7:54543/149‑1 (MQ=255)
                                                                                                                                gtggCCTTGGCTGCTGGCCGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCTGTTGTTCATCATGCTCGGTGCTTTGCTGCACTGGCGCCCACGCAGCTTCCTTGACGCGGTGgc                 <  7:58920/149‑1 (MQ=255)
                                                                                                                                  ggCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCTGTTGTTCATCATGCTCGGTGCTTTGCTGCACTGGCGcc                                          >  6:257667/1‑122 (MQ=255)
                                                                                                                                  ggCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCTGTTGTTCATCATGCTCGGTGCTTTGCTGCACTGGCGcc                                          <  5:257667/122‑1 (MQ=255)
                                                                                                                                     cTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCTGTTGTTCATCATGCTCGGTGCTTTGCTGCACTGGCGCCCACGCAGCTTCCTTGACGCGGTGGCGCGTg            >  8:281594/1‑149 (MQ=255)
                                                                                                                                        gtctgctgGCTGGCTGTATCATGAGTCTGCGACGAAACCTCCGATTACCGCCACCCCGGGGCTCAACACCTACAACCGGGTGTTCATCATGCTCGGTGCTTTGCGGCACTGGCGCCCACGCAGCTTCCTTGACGCGGGGGCGCGTGc           <  6:184369/145‑1 (MQ=255)
                                                                                                                                        ggCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCtgtt                                                                             >  6:302835/1‑81 (MQ=255)
                                                                                                                                        ggCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCtgtt                                                                             <  5:302835/81‑1 (MQ=255)
                                                                                                                                        ggCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCTGTTGTTCATCATGCTCGGTGCTTTGCTGCACTGGCGCCCACGCAGCTTCCTTGACGCGGTGGCGCGTGc           <  4:244703/147‑1 (MQ=255)
                                                                                                                                                gctggctgTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCTGTTGTTCATCATGCTCGGTGCTTTGCTGCACTGGCGCCCACGCAGCTTCCTTGACGCGGTGGCGCGTGCGGTACCGac  >  2:206095/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGGGCCCGAACAGCGCCCGTAGCGCAGAAGACTGCGGTGTCGACCCCAGCTTCACAGCGCCACCCGCGCCGCAACGCACGCGCCCGGGAGAGTGGCTGGAACACAGCCCGATCCTTATCCTGCTGTTGGTGGCCTTGGCTGCTGGCTGGCTGTATCAGGAGTTTGCGACGAAACCTGCGATTACCGCCATCTCGGGGCTCAACACCTACAACCTGTTGTTCATCATGCTCGGTGCTTTGCTGCACTGGCGCCCACGCAGCTTCCTTGACGCGGTGGCGCGTGCGGTACCGAC  >  NC_002947/3535523‑3535814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑