| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,542,321 | C→T | 6.7% | P134P (CCG→CCA) | galE ← | UDP‑glucose 4‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,542,321 | 0 | C | T | 6.7% | 172.4 / 2.5 | 60 | P134P (CCG→CCA) | galE | UDP‑glucose 4‑epimerase |
| Reads supporting (aligned to +/- strand): ref base C (28/27); new base T (2/2); total (31/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
CGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACGTT > NC_002947/3542183‑3542464 | cGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCGTATGGGTTGagca < 4:55218/149‑4 (MQ=255)cGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATc < 3:189184/148‑1 (MQ=255) ggCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCATCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCgg > 2:107601/1‑149 (MQ=255) gCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGGCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGc < 2:328856/149‑1 (MQ=255) aCGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTg < 2:272918/148‑1 (MQ=255) tCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTg < 2:320777/144‑1 (MQ=255) tCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTg > 1:320777/1‑144 (MQ=255) tCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGATCAACCCGTATGGGTTGagcaaat < 6:329509/139‑7 (MQ=255) tCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGATCAACCCGTATGGGTTGagcaaat > 5:329509/1‑133 (MQ=255) cTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCat < 4:273450/149‑1 (MQ=255) tGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatc > 3:165620/1‑149 (MQ=255) cTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatcgatc < 2:326559/149‑1 (MQ=255) cTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatcgatc > 4:16455/1‑149 (MQ=255) ccGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCATGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGAACGGCCCCTTGGTGTGTGCTTCATCGATCgg < 1:28724/148‑1 (MQ=255) ccGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGc < 4:165620/149‑1 (MQ=255) ggTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGgt < 5:218666/127‑1 (MQ=255) ggTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGgt > 6:218666/1‑127 (MQ=255) gTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATAc < 4:280124/106‑1 (MQ=255) gTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATAc > 3:280124/1‑106 (MQ=255) gcgcCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCTTCGATCGGCTCGTACTg > 3:245452/1‑149 (MQ=255) cTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTg > 2:272922/1‑149 (MQ=255) ttGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGta > 8:66439/1‑149 (MQ=255) aaaTAGCGCAGGCTTACCGACTTCAAACCATACGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGtata < 3:16455/148‑1 (MQ=255) aaTAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcg < 6:6194/149‑1 (MQ=255) aGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcggcg > 4:19226/1‑149 (MQ=255) cgcAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATAcggcgg > 5:184073/1‑148 (MQ=255) gcAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCa > 4:58879/1‑149 (MQ=255) cTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCga < 6:184073/149‑1 (MQ=255) ttACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGt > 1:112496/1‑132 (MQ=255) ttACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGt < 2:112496/132‑1 (MQ=255) cGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGCCCATCCATTTGCTCAACCCAAACTGGTTGATCGGCCCCATGGTGTGTGCTTCATCGATCCGCACGTACTGCGGGTTCCCGAATACGGCCGCACTCgaagaa > 5:105673/1‑148 (MQ=255) ttCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATAAGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCCATCGGCCCGAACTGCGGGTTGCCGTATACGGCTGCACTCCAAGAAAaca > 7:172112/1‑148 (MQ=255) caaaGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGc > 2:278571/4‑149 (MQ=255) gCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGAt < 1:272922/149‑1 (MQ=255) gAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCAt < 1:283719/149‑1 (MQ=255) aaGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCAtt < 3:58879/149‑1 (MQ=255) tCAGGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCAt > 8:201238/1‑149 (MQ=255) tCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAaca > 1:290887/1‑121 (MQ=255) tCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAaca < 2:290887/121‑1 (MQ=255) cAATAATCTGCTCGACAATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATAGATCGGCACGTACTGCgg > 4:48039/1‑88 (MQ=255) cAAGAATCTTCTCGACCATCCATTTGCTCAACCCATACGTGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGTCACGTACTGCgg < 3:48039/88‑1 (MQ=255) aaGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGaaaa > 8:119990/1‑117 (MQ=255) aaGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGaaaa < 7:119990/117‑1 (MQ=255) gACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTg < 8:44575/141‑1 (MQ=255) gACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTg > 7:44575/1‑141 (MQ=255) tccatTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatcgat < 2:283283/55‑1 (MQ=255) tccatTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCatcgat > 1:283283/1‑55 (MQ=255) tccatTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAgg < 7:66439/149‑1 (MQ=255) catTTGCTCAACCCATTCGGGTTGATCTGCCCCTTGGTGTGTGCGTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCTGGTGCCTGATGCCGGCATTGACCATGTCCTGCAGCAGGGAAAGGGt < 8:172112/149‑1 (MQ=255) atTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTg > 6:183918/1‑149 (MQ=255) atTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTg > 3:168111/1‑149 (MQ=255) tGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGc < 8:153728/104‑1 (MQ=255) tGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGc > 7:153728/1‑104 (MQ=255) gCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGCCCTGCAGCAGGGAAAGGGTGGCTg > 5:178234/1‑149 (MQ=255) cTCAACCCATACTGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTTGCTg < 6:105673/148‑1 (MQ=255) atCAACCCGTATGGGTTGATCGGCCCCTTGGAGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGCTGCCTGATTCAGGCAATGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGc > 5:254398/2‑149 (MQ=255) tCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGc > 2:79716/1‑148 (MQ=255) tCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGAACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCATGCAGCAGGGAAAGGGTGGCTGc > 3:224526/1‑148 (MQ=255) cAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCAc > 7:201047/1‑51 (MQ=255) cAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCAc < 8:201047/51‑1 (MQ=255) aaCCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGAc > 3:204022/1‑149 (MQ=255) ccATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACgtt < 4:168111/149‑1 (MQ=255) | CGGCTCGTGACGCTCGCCTAGCTGGCCTTCCGGGTCAGCGCCCGCTGCATTGAAATAGCGCAGGCTTACCGACTTCAAACCATAGGCCCGGTCGAAGTCCTCAAGAATCTGCTCGACCATCCATTTGCTCAACCCATACGGGTTGATCGGCCCCTTGGTGTGTGCTTCATCGATCGGCACGTACTGCGGGTTGCCGTATACGGCGGCACTCGAAGAAAACACCAGGTGCCTGATGCCGGCATTGACCATGGCCTGCAGCAGGGAAAGGGTGGCTGCGACGTT > NC_002947/3542183‑3542464 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |