| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,681,805 | C→T | 100% | intergenic (‑154/‑31) | PP_3247 ← / → PP_3248 | bile acid/Na+ symporter family transporter/Dyp‑type peroxidase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,681,805 | 0 | C | T | 95.9% | 69.2 / ‑4.3 | 24 | intergenic (‑154/‑31) | PP_3247/PP_3248 | bile acid/Na+ symporter family transporter/Dyp‑type peroxidase family protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (13/10); total (14/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCTCGGGCGAAGGATCTGAAGCAGTGTAGGTCGGCCTTGATTGATAGCTAATTGCAAAGGGCCAGCTCTTGGTAACCGTTTGTTACCACGTGCAGCGGCTGGCGAGGCTGCATGCCGCATTGCAGCAGGCATGCTGGCGAAGTAGCATTCCGTTTTTACCTTAGGATTAGCCTGATATGCCGTTCCAGCAAGGTCTGCTTGCCACCCCGGTGCCGGCCCACGCCCGTCACCTGTTCTTCACCCTGCAGTCGCCCGAGGCGTTGCCTGCCGCGCTGGATGCCTTGCTGCCG > NC_002947/3681660‑3681949 | tctcGGGCGAAGGATCTGAAGCAGTGTAGGTCGGCCTTGATTGATAGCTAATTGCAAAGGGCCAGCTCTTGGTAACCGTTTGTTACCACGTGCAGCGGCTGGCGAGGCTGCATGCCGCATTGCAGCAGGCATGCTGGCGAAGTAGTAt < 1:18216/148‑1 (MQ=255) tcGGGCGAAGGATCTGAAGCAGTGTAGGTCGGCCTTGATTGATAGCTAATTGCAAAGGGCCAGCTCTTGGTAACCGTTTGTTACCACGTGCAGCGGCTGGCGAGGCTGCATGCCGCATTGCAGCAGGCATGCTGGCGAAGTAGTATTcc > 3:63825/1‑149 (MQ=255) ggCGAAGGATCTGAAGCAGTGTAGGTCGGCCTTGATTGATAGCTAATTGCAAAGGGCCAGCTCTTGGTAACCGTTTGTTACCACGTGCAGCGGCTGGCGAGGCTGCATGCCGCATTGCAGCAGGCATGCTGGCGAAGTAGTATTCCGtt < 8:91571/149‑1 (MQ=255) tCTGAAGCAGTGTAGGTCGGCCTTGATTGATAGCTAATTGCAAAGGGCCAGCTCTTGGTAACCGTTTGTTACCACGTGCAGCGGCTGGCGAGGCTGCATGCCGCATTGCAGCAGGCATGCTGGCGAAGTAGCATTCCGTTTTTACCt > 4:223880/1‑147 (MQ=255) aGTGTAGGTCGGCCTTGATTGATAGCTAATTGCAAAGGGCCAGCTCTTGGTAACCGTTTGTTACCACGTGCAGCGGCTGGCGAGGCTGCATGCCGCATTGCAGCAGGCATGCTGGCGAAGTAGTATTCCGTTTTTACCTTAGGATTAGc < 3:195850/149‑1 (MQ=255) tCGGCCTTGATTGATAGCTAATTGCAAAGGGCCAGCTCTTGGTAACCGTTTGTTACCACGTGCAGCGGCTGGCGAGGCTGCATGCCGCATTGCAGCAGGCATGCTGGCGAAGTAGTATTCCGTTTTTACCTTAGGATTAGCCTGATATg < 1:186408/149‑1 (MQ=255) gattgatAGCTAATTGCAAAGGGCCAGCTCTTGGTAACCGTTTGTTACCACGTGCAGCGGCTGGCGAGGCTGCATGCCGCATTGCAGCAGGCATGCTGGCGAAGTAGTATTCCGTTTTTACCTTAGGATTAGCCTGATATGCCGTTc < 1:289139/147‑1 (MQ=255) gatATCTAAATGCAAAGGGCCAGCTCTTTGTAACCGTTTCTTACCACGTGCAGCGGCTGGCGAGGCTGCATGCCTGATTGCAGCAGGCATGCTGTCGAAGTAGTATTCCGTTTTTACCTTAGGATTAGCCTGATATTCCGTTCCAGCa < 8:85214/148‑1 (MQ=255) gCAAAGGGCCAGCTCTTGGTAACCGTTTGTTACCACGTGCAGCGGCTGGCGAGGCTGCATGCCGCATTGCAGCAGGCATGCTGGCGAAGTAGTATTCCGTTTTTACCTTAGGATTAGCGTGATATGCCGTTCCAGCAAGGTCTGCTTg > 6:163302/1‑148 (MQ=255) ccAGCTCTTGGTAACCGTTTGTTACCACGTGCAGCGGCTGGCGAGGCTGCATGCCGCATTGCAGCAGGCATGCTGGCGAAGTAGTATTCCGTTTTTACCTTAGGATTAGCCTGATATGCCGTTCCAGCAAGGTCTGCTTGCCCCCCCgg > 5:314167/1‑149 (MQ=255) ccAGCTCTTGGTAACCGTTTGTTACCACGTGCAGCGGCTGGCGAGGCTGCATGCCGCATTGCAGCAGGCATGCTGGCGAAGTAGTATTCCGTTTTTACCTTAGGATTAGCCTGATATGCCGTTCCAGCAAGGTCTGCTTGCCACCCCgg > 5:205665/1‑149 (MQ=255) tctTGGTAACCGTTTGTTACCACGTGCAGCGGCTGGCGAGGCTGCATGCCGCATTGCAGCAGGCATGCTGGCGAAGTAGTATTCCGTTTTTACCTTAGGATTAGCCTGATATGCCGTTCCAGCAAGGTCTGCTTGCCACCCCGGTGCCg > 3:222722/1‑149 (MQ=255) ctTGGTAACCGTTTGTTACCACGTGCAGCGGCTGGCGAGGCTGCATGCCGCATTGCAGCAGGCATGCTGGCGAAGTAGTATTCCGTTTTTAGCTTAGGATTAGCCTGATATGCCGTTCCAGCAAGGTCTGCTTGCCACCCCGGTGCCgg > 4:85685/1‑149 (MQ=255) ggTAACCGTTTGTTACCACGTGCAGCGGCTGGCGAGGCTGCATGCCGCATTGCAGCAGGCATGCTGGCGAAGTAGTATTCCGTTTTTACCTTAGGATTAGTCTGATATGCCGTTCCAGCAAGGTCTGCTTGCCACCCCGGTGcca > 1:116376/1‑144 (MQ=255) ggTAACCGTTTGTTACCACGTGCAGCGGCTGGCGAGGCTGCATGCCGCATTGCAGCAGGCATGCTGGCGAAGTAGTATTCCGTTTTTACCTTAGGATTAGTCTGATATGCCGTTCCAGCAAGGTCTGCTTGCCACCCCGGTGcca < 2:116376/145‑2 (MQ=255) cACGTGCAGCGGCTGGCGAGGCTGCATGCCGCATTGCAGCAGGCATGCTGGCGAAGTAGTATTCCGTTTTTACCTTAGGATTAGCCTGATATGCCGTTCCAGCAAGGTCTGCTTGCCACCCCGGTGCCGGCCCACGCCCGTCACCTGtt > 4:13915/1‑149 (MQ=255) aCGTGCAGCGGCTGGCGAGGCTGCATGCCGCATTGCAGCAGGCATGCTGGCGAAGTAGTATTCCGTTTTTACCTTAGGATTAGCCTGATATGCCGTTCCAGCAAGGTCTGCTTGCCACCCCGGTGCCGGCCCACGCCCGTCACCTGttc > 6:123266/1‑149 (MQ=255) cTGGCGAGGCTGCATGCCGCATTGCAGCAGGCATGCTGGCGAAGTAGTATTCCGTTTTTACCTTAGGATTAGCCTGATATGCCGTTCCAGCAAGGTCTGCTTGCCACCCCGGTGCCGGCCCACGCCCGTCACCTGTTCTTCACCCTGCa < 3:13915/149‑1 (MQ=255) gAGGCTGCATGCCGCATTGCAGCAGGCATGCTGGCGAAGTAGTATTCCGTTTTTACCTTAGGATTAGCCTGATATGCCGTTCCAGCAAGGTCTGCTTGCCACCCCGGTGCCGGCCCACGCCCGTCACCTGTTCTTCACCCTGCAGTCGc > 4:216638/1‑149 (MQ=255) cATGCTGGCGAAGTAGTATTCCGTTTTTACCTTAGGATTAGCCTGATATGCCGTTCCAGCAAGGTCTGCTTGCCACCCCGGTGCCGGCCCACGCCCGTCACCTGTTCTTCACCCTGCAGTCGCCCGAGGCGTTGCCTGCCGCGCTGGAt < 4:63825/149‑1 (MQ=255) aTGCTGGCGAAGTAGTATTCCGTTTTTACCTTAGGATTAGCCTGATATGCCGTTCCAGCAAGGTCTGCTTGCCACCCCGGTGCCGGCCCACGCCCGTCACCTGTTCTTCATCCTGCAGTCGCCCGAGGCGTTGCCTGCCGCGCTGGATg < 6:205665/149‑1 (MQ=255) tGGCGAAGTAGTATTCCGTTTTTACCTTAGGATTAGCCTGATATGCCGTTCCAGCAAGGTCTGCTTGCCACCCCGGTGCCGGCCCACGCCCGTCACCTGTTCTTCACCCTGCAGTCGCCCGAGGCGTTGCCTGCCGCGCTGGATGCCtt > 2:146958/1‑149 (MQ=255) gCGAAGTAGTATTCCGTTTTTACCTTAGGATTAGCCTGATATGCCGTTCCAGCAAGGTCTGCTTGCCACCCCGGTGCCGGCCCACGCCCGTCACCTGTTCTTCACCCTGCAGTCGCCCGAGGCGTTGCCTGCCGCGCTGGATGCCTtgc > 7:73002/1‑149 (MQ=255) agtagtATTCCGTTTTTACCTTAGGATTAGCCTGATATGCCGTTCCAGCAAGGTCTGCTTGCCACACCGGTGCCGGCCAACGACCGTCACCTGTTCTTCACCCTGCAGTCGCCCGAGGCGTTGCCTGCCGCGCTGGATGCCTTGCTGcc > 5:98266/1‑149 (MQ=255) gtagtaTTCCGTTTTTACCTTAGGATTAGCCTGATATGCCGTTCCAGCAAGGTCTGCTTGCCACCCCGGTGCCGGCCCACGCCCGTCACCTGTTCTTCACCCTGCAGTCGCCCGAGGCGTTGCCTGCCGCGCTGGATGCCTTGCTGCCg > 7:104405/1‑149 (MQ=255) tAGTATTCCGTTTTTACCTTAGGATTAGCCTGATATGCCGTTCCAGCAAGGTCTGCTTGCCACCCCGGTGCCGGCCCACGCCCGTCACCTGTTCTTCACCCTGCAGTCGCCCGAGGCGTTGCCTGCCGCGCTGAATGCCTTGCTGCCg > 5:208134/1‑148 (MQ=255) | TCTCGGGCGAAGGATCTGAAGCAGTGTAGGTCGGCCTTGATTGATAGCTAATTGCAAAGGGCCAGCTCTTGGTAACCGTTTGTTACCACGTGCAGCGGCTGGCGAGGCTGCATGCCGCATTGCAGCAGGCATGCTGGCGAAGTAGCATTCCGTTTTTACCTTAGGATTAGCCTGATATGCCGTTCCAGCAAGGTCTGCTTGCCACCCCGGTGCCGGCCCACGCCCGTCACCTGTTCTTCACCCTGCAGTCGCCCGAGGCGTTGCCTGCCGCGCTGGATGCCTTGCTGCCG > NC_002947/3681660‑3681949 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |