| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 354,548 | T→A | 8.9% | P208P (CCA→CCT) | cbcW ← | choline/betaine/carnitine ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 354,548 | 0 | T | A | 8.9% | 122.1 / 3.1 | 45 | P208P (CCA→CCT) | cbcW | choline/betaine/carnitine ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base T (20/21); new base A (2/2); total (22/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01 | |||||||||||
CCAGGCCCGCTTCGAAGCCCAGGGAGATATCGGCGGTGTTCAGTGCGTTGACCACAGGTTTGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCATCAACTCTTGCGGCACGTCGCAGATGCCCAGGTAGGTCAGGCGAATGGGCGCAGCAATGGCGAACACCAC > NC_002947/354400‑354691 | ccAGGCCCGCTTCGAAGCCCAGGGAGATATCGGCGGTGTTCAGTGCGTTGACCACAGGTTTGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCtt < 2:272164/149‑1 (MQ=255)ccAGGCCCGCTTCGAAGCCCAGGGAGATATCGGCGGTGTTCAGTGCGTTGACCACAGGTTTGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCtt < 7:266740/149‑1 (MQ=255)ccAGGCCCGCTTCGAAGCCCAGGGAGATATCGGCGGTGTTCAGTGCGTTGACCACAGGTTTGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCtt < 4:195795/149‑1 (MQ=255)ccAGGCCCGCTTCGAAGCCCAGGGAGATATCGGCGGTGTTCAGTGCGTTGACCACAGGTTTGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCtt < 5:24844/149‑1 (MQ=255)ccAGGCCCGCTTCGAAGCCCAGGGAGATATCGGCGGTGTTCAGTGCGTTGACCACAGGTTTGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCtt < 4:5465/149‑1 (MQ=255) cAGGCCCGCTTCGAAGCCCAGGGAGATATCGGCGGTGTTCAGTGCGTTGACCACAGGTTTGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTg < 1:74618/149‑1 (MQ=255) cAGGCCCGCCTCGCACCCCACGGACATATCGGCGGTGTTCCGTGCCTTGACCACAGGTGTGCCCAGGCCATCAGCGCCAACCATGGCGGCAATGCC‑CACCCTCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTg < 2:119222/149‑1 (MQ=255) ccGCTTCGAAGCCCAGGGAGATATCGGCGGTGTTCAGTGCGTTGACCACAGGTTTGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATc < 1:120806/149‑1 (MQ=255) ccGCTTCGAAGCCCAGGGAGATATCGGCGGTGTTCAGTGCGTTGACCACAGGTTTGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATc > 3:181499/1‑149 (MQ=255) gAAGCCCAGGGGGATATCGGCGGTGTTCAGTGCGTTGACCACAGGTTTGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGc < 5:27480/148‑1 (MQ=255) cAGGGAGATATCGGCGGTGTTCAGTGCGTTGACCACAGGTTTGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCTGCGATGCTTGGCATCGCGTGCGGCAGtt < 4:181499/149‑1 (MQ=255) tCGGCGGTGTTCAGTGCGTTGACCACAGGTTTGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACgg > 1:198308/1‑147 (MQ=255) tCGGCGGTGTTCAGTGCGTTGACCACAGGTTTGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACgg < 2:198308/147‑1 (MQ=255) gcggTGTTCAGTGCGTTGACCACAGGTTTGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAGTTGGTTCGCGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGt > 1:110598/1‑148 (MQ=255) ttCAGTGCGTTGACCACAGGTTTGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCt > 1:36411/1‑148 (MQ=255) cAGTGCGTTGACCACAGGTTTGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTgac > 1:295516/1‑149 (MQ=255) acaGGTTTGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGagcagc > 2:300382/1‑148 (MQ=255) gTTTGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCCAAGCATcgccg > 1:2112/1‑100 (MQ=255) gTTTGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCCAAGCATcgccg < 2:2112/102‑3 (MQ=255) tttGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAgg > 3:282134/1‑149 (MQ=255) cccAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGAGGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCtt < 2:309018/149‑1 (MQ=255) cAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGc < 8:61966/149‑1 (MQ=255) gCCATGAGCGCCAACCAGGGCGTCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGc < 2:197871/141‑1 (MQ=255) gCCATCAGCGCCAACCAGGGCGGCAATGACGCACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGCCATCGCGTCCGGCAGTTCGATACGGGTAAGCAGCTCACGACCCGAGCAGCCAAAGGc > 1:197871/1‑142 (MQ=255) aTCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGt < 1:300382/149‑1 (MQ=255) aaCCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCa < 2:297754/143‑1 (MQ=255) aaCCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCa > 1:297754/1‑143 (MQ=255) aGGGCGGCAATGAC‑CACCTTCGACTGCGACAGCATGATGCTTTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATGGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCATCAACTGTTg < 4:282134/149‑1 (MQ=255) gggCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCATCAACTCTTGc > 6:79821/1‑149 (MQ=255) cAATAAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATTCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACTACGCGAGCAGCCAAAGGCCTTGCCGGc < 2:218717/127‑1 (MQ=255) cgacagcgacagCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCATCAACTCTTGCGGCACGTCGCAGATGcc > 2:85781/1‑147 (MQ=255) acagcgacagcATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCATCAACTCTTGCGGCACGTCGCAGATGCCCagg > 1:14544/1‑149 (MQ=255) agcgacagcATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCATCAACTCTTGCGGCACGTCGCAGATGCCCaggta < 4:150196/149‑1 (MQ=255) cATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCATCAACTCTTGCGGCACGTCGCAGATGCCCAGGTAGGTCAGGc < 3:210053/149‑1 (MQ=255) cATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCATCAACTCTTGCGGCACGTCGCAGATGCCCAGGTAGGTCAGGc > 8:247298/1‑149 (MQ=255) atgCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCATCAACTCTTGCGGCACGTCGCAGATGCCCAGGTAGGTCAGGCGaa < 2:295516/148‑1 (MQ=255) cATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAgg < 6:41009/85‑1 (MQ=255) cATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAgg > 5:41009/1‑85 (MQ=255) cATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCATCAACTCTTGCGGCACGTCGCAGATGCCCAGGTAGGTCAGGCGAATggg < 2:175006/149‑1 (MQ=255) cATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCATCAACTCTTGCGGCACGTCGCAGATGCCCAGGTAGGTCAGGCGAATggg < 2:14544/149‑1 (MQ=255) cATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCATCAACTCTTGCGGCACGTCGCAGATGCCCAGGTAGGTCAGGCGAATgg < 1:85781/148‑1 (MQ=255) cac‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCATCAACTCTTGCGGCACGTCGCAGATGCCCAGGTAGGTCAGGCGAATGGGCGCAGCAATg > 3:42314/1‑149 (MQ=255) c‑cGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCATCAACTCTTGCGGCACGTCGCAGATGCCCAGGTAGGTCAGGCGAATGGGCGCAGCAATGGc < 5:79821/149‑1 (MQ=255) cggcggCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCATCAACTCTTGCGGCACGTCGCAGATGCCCAGGTAGGTCAGGCGAATGGGCGCAGCAATGGc > 2:190679/1‑148 (MQ=255) ggcggcGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCATCAACTCTTGCGGCACGTCGCAGATGCCCAGGTAGGTCAGGCGAAt > 8:286217/1‑132 (MQ=255) gcggcgATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGTAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCATCAACTCTTGCGGCACGTCGCAGATGCCCAGGTAGGTCAGGCGAATGGGCGCAGCAATGGCGaa > 1:184461/1‑149 (MQ=255) gcggcgATGCTTGGCATCGCGTGCGGCAGTTCGAAACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCATCAACTCTTGCGGCACGTCGCAGATGCCCAGGTAGGTCAGGCGAAt > 3:126190/1‑131 (MQ=255) cgcgATGCCAAGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGc > 1:276713/2‑69 (MQ=255) cgcgATGCCAAGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGc < 2:276713/68‑1 (MQ=255) aTGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCATCAACTCTTGCGGCACGTCGCAGATGCCCAGGTAGGTCAGGCGAATGGGCGCAGCAATGGCGAAcaccac > 4:257653/1‑149 (MQ=255) | CCAGGCCCGCTTCGAAGCCCAGGGAGATATCGGCGGTGTTCAGTGCGTTGACCACAGGTTTGCCCAGGCCATCAGCGCCAACCAGGGCGGCAATGAC‑CACCATCGACAGCGACAGCATGATGCATTGAG‑TGACAC‑CGGCGGCGATGCTTGGCATCGCGTGCGGCAGTTCGATACGGGTAAGCAGCTGACGACGCGAGCAGCCAAAGGCCTTGCCGGCGTCCATCAACTCTTGCGGCACGTCGCAGATGCCCAGGTAGGTCAGGCGAATGGGCGCAGCAATGGCGAACACCAC > NC_002947/354400‑354691 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |