Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,747,964 A→T 8.0% L266Q (CTG→CAG)  kefB‑II ← glutathione‑regulated potassium/H+ antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,747,9640AT 8.0% 144.0 / 2.9 50L266Q (CTG→CAG) kefB‑IIglutathione‑regulated potassium/H+ antiporter
Reads supporting (aligned to +/- strand):  ref base A (24/22);  new base T (2/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

ACAGCGCCTTGACCACCATCAGCGCCAGCACGCCAGAGCTGATGAGCAGCCAGTTCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTCCATCAGCAGCGCTGCTCCTAGCACCACCAGCAGCGCCGCCGCAGTCATCACCTCGCGCGCCCTG  >  NC_002947/3747818‑3748107
                                                                                                                                                  |                                                                                                                                                
acaGCGCCTTGACCACCATCAGCGCCAGCACGCCAGAGCTGATGAGCAGCCAGTTCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGc                                                                                                                                                >  2:313231/1‑149 (MQ=255)
      ccTTGACCACCATCAGCGCCAGCACGCCAGAGCTGATGAGCAGCCAGTTCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCtggtgg                                                                                                                                          >  8:190356/1‑149 (MQ=255)
        ttGACCACCATCAGCGCCAGCACGCCAGAGCTGATGAGCAGCCAGTTCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCg                                                                                                                                        >  4:281401/1‑149 (MQ=255)
             caccaTCAGCGCCAGCACGCCAGAGCTGATGAGCAGCCAGTTCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAgg                                                                                                                                   >  4:261995/1‑149 (MQ=255)
             caccaTCAGCGCCAGCACGCCAGAGCTGATGAGCAGCCAGTTCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAgg                                                                                                                                   >  6:23742/1‑149 (MQ=255)
             caccaTCAGCGCCAGCACGCCACAGCTGATGAGCAGCCAGTTCCTGGCCACCACCTGCAGGACCAACGCCATCCCGACGCCGAAAAAGAACAGCCCCAGAAACAGACCCCGAAAAGGTACTATGTCAACAACCACATGGAGGCGGAAgg                                                                                                                                   >  8:46830/1‑149 (MQ=255)
               ccaTCAGCGCCAGCACGCCAGAGCTGATGAGCAGCCAGTTCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTg                                                                                                                                 <  7:190356/149‑1 (MQ=255)
               ccaTCAGCGCCAGCACGCCAGAGCTGATGAGCAGCCAGTTCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTg                                                                                                                                 <  3:281401/149‑1 (MQ=255)
               ccaTCAGCGCCAGCACGCCAGAGCTGATGAGCAGCCAGTTCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTg                                                                                                                                 <  8:166715/149‑1 (MQ=255)
                  tCAGCGCGAGCACGCCAGAGGGGATGAGCAGCCAGTTCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGAc                                                                                                                              <  7:46830/149‑1 (MQ=255)
                  tCAGCGCCAGCACGCCAGAGCTGATGAGCAGCCAGTTCCTGGCCACCACATGCAGGTCCAGCGCCATGCCGACGGCGAGAAAGAACCGCCCCAGTACCAGTCCGCGAAACGGTTATAAGTCAACCTCCAGCTGGTGGCGGAAGGTGGAc                                                                                                                              >  1:242519/1‑149 (MQ=255)
                    aGCGCCAGCACGCCAGAGCTGATGAGCAGCCAGTTCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTc                                                                                                                            <  1:242234/149‑1 (MQ=255)
                           gCACGCCAGAGCTGATGAGCAGCCAGTTCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGAcag                                                                                                                      >  2:159702/1‑148 (MQ=255)
                                ccAGAGCTGATGAGCAGCCAGTTCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAgcagca                                                                                                                 >  4:302918/1‑148 (MQ=255)
                                 cAGAGCTGATGAGCAGCCAGTTCCTGGCCACCACCTGCAGATCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGcaca                                                                                                               >  6:125215/1‑149 (MQ=255)
                                      cTGATGAGCAGCCAGTTCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCgg                                                                                                           >  3:263911/1‑148 (MQ=255)
                                        gatgaGCAGCCAGTTCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGcaa                                                                                                        >  4:36577/1‑149 (MQ=255)
                                               agcCAGTTCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGc                                                                                                 >  3:106397/1‑149 (MQ=255)
                                                 ccAGTTCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑c                                                                                              <  2:293604/149‑1 (MQ=255)
                                                  cAGTTCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑cc                                                                                             <  3:302918/149‑1 (MQ=255)
                                                  cAGTTCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑cc                                                                                             <  1:159702/149‑1 (MQ=255)
                                                      tCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑Cccat                                                                                          <  4:263911/148‑1 (MQ=255)
                                                            ccaccaCCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑Cccatcgccatc                                                                                   <  7:79608/149‑1 (MQ=255)
                                                                     gCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGgccg                                                                          >  4:283613/1‑149 (MQ=255)
                                                                            cAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGAtt                                                                    <  3:283613/148‑1 (MQ=255)
                                                                              gcgcCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTcc                                                                 <  3:36577/149‑1 (MQ=255)
                                                                              gcgcCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTcc                                                                 <  3:261995/149‑1 (MQ=255)
                                                                              gcgcCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTcc                                                                 <  5:23742/149‑1 (MQ=255)
                                                                                    tGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCACCACCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTCCATcag                                                            >  2:121953/1‑148 (MQ=255)
                                                                                      ccGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGAt                                                                     <  6:264972/137‑1 (MQ=255)
                                                                                      ccGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGAt                                                                     >  5:264972/1‑137 (MQ=255)
                                                                                       cGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACCGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTCCATCagcagc                                                        <  5:280349/149‑1 (MQ=255)
                                                                                          cGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTCCATCAGCAGCgct                                                     >  6:177900/1‑149 (MQ=255)
                                                                                              gagaAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCTGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTCCATCAGCAGCGCTGCTc                                                 >  8:128667/1‑149 (MQ=255)
                                                                                              gagaAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCTGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTCCATCAGCAGCGCTGCTc                                                 >  8:133030/1‑149 (MQ=255)
                                                                                              gagaAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCTCCCCATCGCCATCGACAGGCCGCCGATTTCCATCAGCAGCgctgct                                                  <  1:234031/149‑1 (MQ=255)
                                                                                              gagaAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTCCATCAGCAGCGCTGCTc                                                 >  8:80980/1‑149 (MQ=255)
                                                                                                 aaagaaCAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTCCATCAGCAGCGCTGCTcc                                                >  1:92255/1‑147 (MQ=255)
                                                                                                     aaCAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGc                                                                                                 <  2:209592/95‑1 (MQ=255)
                                                                                                     aaCAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGc                                                                                                 >  1:209592/1‑95 (MQ=255)
                                                                                                          ccccAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTCCATCAGCAGCGCTGCTcc                                                <  2:97939/138‑1 (MQ=255)
                                                                                                          ccccAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTCCATCAGCAGCGCTGCTcc                                                >  1:97939/1‑138 (MQ=255)
                                                                                                          ccccAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTCCATCAGCAGCGCTGCTCCTAGCAccacca                                     <  5:125215/149‑1 (MQ=255)
                                                                                                             cAGTAACCGTCCGCGAAACGGTTCTATGTCAACCTCCTGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTCCATCAGCAGCGCTGCTCCTAGCACCACcagca                                  <  7:133030/149‑1 (MQ=255)
                                                                                                             cAGTAACAGTCCGCGAAACGGTTCTATCTCAACCTCCTGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTCCATCAGCAGCGCTGCTCCTAGCACCACcagca                                  <  7:128667/149‑1 (MQ=255)
                                                                                                                  aCAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTCCATCAGCAGCGCTGCTCCTAGCACCACCAGCAGcgcc                             <  4:106397/149‑1 (MQ=255)
                                                                                                                      tCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTCCATCAGCAGCGCTGCTCCTAGCACCACCAGCAGcgccgccg                         >  5:329963/1‑149 (MQ=255)
                                                                                                                           gAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTCCATCAGCAGCGCTGCTCCTAGCACCACCAGCAGCGCCGCCGCAGtc                    <  5:177900/149‑1 (MQ=255)
                                                                                                                                  ttCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTCCATCAGCAGCGCTGCTCCTAGCACCACCAGCAGCGCCGCCGCAGTCATCACCt             >  5:123255/1‑149 (MQ=255)
                                                                                                                                             ccTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTCCATCAGCAGCGCTGCTCCTAGCACCACCAGCAGCGCCGCCGCAGTCATCACCTCGCGCGCCCTg  >  3:165278/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ACAGCGCCTTGACCACCATCAGCGCCAGCACGCCAGAGCTGATGAGCAGCCAGTTCCTGGCCACCACCTGCAGGTCCAGCGCCATGCCGACGCCGAGAAAGAACAGCCCCAGTAACAGTCCGCGAAACGGTTCTATGTCAACCTCCAGCTGGTGGCGGAAGGTGGACTCCGACAGCAGCACACCGGCAACAAAAGCT‑CCCATCGCCATCGACAGGCCGCCGATTTCCATCAGCAGCGCTGCTCCTAGCACCACCAGCAGCGCCGCCGCAGTCATCACCTCGCGCGCCCTG  >  NC_002947/3747818‑3748107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑