| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,753,595 | G→T | 13.1% | A96A (GCC→GCA) | PP_3316 ← | chaperone‑associated ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,753,595 | 0 | G | T | 13.1% | 161.9 / 12.9 | 60 | A96A (GCC→GCA) | PP_3316 | chaperone‑associated ATPase |
| Reads supporting (aligned to +/- strand): ref base G (28/23); new base T (4/4); total (32/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
ACGCTTGGCGGCTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGATCATC > NC_002947/3753454‑3753738 | aCGCTTGGCGGCTGCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACgg < 7:292172/149‑1 (MQ=255) cGCTTGGCGGCTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGa > 2:291532/1‑149 (MQ=255) gCTTGGCGGCTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGAt < 6:3906/149‑1 (MQ=255) gCTTGGCGGCTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGAt < 6:196175/149‑1 (MQ=255) gCTTGGCGGCTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGAt > 2:261910/1‑149 (MQ=255) gcggcTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTTGCCACGGATGCCGa < 4:217614/149‑1 (MQ=255) gattCCTGCAACTGGGCCTCGGAGTGTTGGCTGATGCGGCTGGCCAGCCCGCTGCTACGGGGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGAGGCCGAGtc < 2:303995/147‑1 (MQ=255) cTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTTGCCACGGATGCCGatgaa < 7:231672/149‑5 (MQ=255) tGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTcc > 8:50335/1‑149 (MQ=255) cAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGAt < 2:207126/133‑1 (MQ=255) cAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGAt > 1:207126/1‑133 (MQ=255) cAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCg < 7:20958/148‑1 (MQ=255) tCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCgcagca > 3:169307/1‑148 (MQ=255) cGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCGAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGtc > 6:92558/1‑130 (MQ=255) cGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGtc < 5:92558/130‑1 (MQ=255) ggAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCCACGGATGCCGAGTCTTCTCCGATGCGCAGCAAAt > 5:254454/1‑149 (MQ=255) gtTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTg > 4:171423/1‑149 (MQ=255) gtTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCAAGACGAAGCTTCAGCCACTTCATCGGCATCCGTGGCAACGCATGCCGAGTCTTCTCCGATCAGCAGCAAAACGCTg > 2:64445/1‑149 (MQ=255) ttCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGc < 4:169307/149‑1 (MQ=255) ttCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCACCGATGCGCAGCAAATCGCTGc < 7:50335/149‑1 (MQ=255) gCTGATGCGGCTGGCCAGCCCGCTGCTGGGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGccc < 6:193001/148‑1 (MQ=255) gCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCa > 2:66226/1‑149 (MQ=255) tGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCAt > 3:309134/1‑148 (MQ=255) aTGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGaa < 2:266936/149‑1 (MQ=255) gCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTc > 7:250025/1‑146 (MQ=255) gCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTc < 8:250025/146‑1 (MQ=255) gCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCg < 1:3160/147‑1 (MQ=255) gctgcGGCGGCGTGGCGTGCCGGACACTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTgc > 5:274954/1‑149 (MQ=255) tggcgtgCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGt < 7:304878/83‑1 (MQ=255) tggcgtgCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGt > 8:304878/1‑83 (MQ=255) tggcgtgCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGaaaa < 4:320090/149‑1 (MQ=255) tggcgtgCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGaaaa < 1:261910/149‑1 (MQ=255) gcgtgCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAgg < 1:291532/149‑1 (MQ=255) gCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCt > 6:54672/1‑148 (MQ=255) cccGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCt < 5:54672/147‑1 (MQ=255) ccGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCTCTTCATCGGCATCCGTCGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAACCCGCTGCGCGAGCCGAAAAGGGCTTc < 6:254454/149‑1 (MQ=255) ccGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTc < 4:309134/149‑1 (MQ=255) ccGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTc < 1:66226/149‑1 (MQ=255) gCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAggg > 2:93671/1‑149 (MQ=255) gCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAggg > 5:236658/1‑149 (MQ=255) ggTTCCCCAGCCGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGt < 6:42839/146‑1 (MQ=255) ggTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGt > 5:42839/1‑146 (MQ=255) cccAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCTCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAACGGCTTCCAGTGGGGAAAGGGTGCgccag > 8:290457/1‑144 (MQ=255) cccAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAACGGGTGCGttttt > 8:214402/1‑149 (MQ=255) ccAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTg > 3:220968/1‑149 (MQ=255) gacgCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGgcg > 7:297138/1‑149 (MQ=255) acgCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGgcg > 4:285403/1‑148 (MQ=255) cTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTcc < 2:168468/116‑1 (MQ=255) cTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTcc > 1:168468/1‑116 (MQ=255) ttCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCAcc > 2:277146/1‑148 (MQ=255) aCTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGACAGGGTGCGTTTTTGCTGGCGCACCAATTgggg > 8:308349/1‑147 (MQ=255) cATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTa < 6:236658/148‑1 (MQ=255) tCGGCATCCGTTGCCACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAg < 1:197472/147‑1 (MQ=255) tCGGCATCCGTTGCCACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAg > 2:197472/1‑147 (MQ=255) tCGGCATCCGTTGCCACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGt > 4:15259/1‑148 (MQ=255) cGGCATCCGTTGCCACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACGCTTCGAACAAACCGCGGCGCGTGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGGTGTTGCTGGCGCGCCAAGTGGCGGTAGGGa > 8:261389/1‑149 (MQ=255) ggCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGa > 5:124320/1‑148 (MQ=255) gCATCCGTTGCCACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAAACGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGatc > 4:29366/1‑149 (MQ=255) gCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGat > 7:44965/1‑148 (MQ=255) tCCGTTGCCACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGatcatc < 3:29366/149‑1 (MQ=255) | ACGCTTGGCGGCTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGATCATC > NC_002947/3753454‑3753738 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |