Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,763,095 A→G 12.1% D113G (GAT→GGT)  PP_3326 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,763,0950AG12.1% 101.4 / 4.8 41D113G (GAT→GGT) PP_3326hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (18/18);  new base G (3/2);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCTGAGCGACCCTCTGCAAGTCGGGCCAGGCAAGACGCTGGAAGTGCAG  >  NC_002947/3762950‑3763243
                                                                                                                                                 |                                                                                                                                                    
ccGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGTTCAATCAGGATCGCACCCATGTCAAGGATGc                                                                                                                                                   <  6:96386/149‑1 (MQ=255)
    tCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCATCCTTGACATGGGTGCGATc                                                                                                                                               >  4:231736/1‑149 (MQ=255)
      aCGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCATCCTTGACATGGGTGCGATcct                                                                                                                                             <  3:231736/149‑3 (MQ=255)
        ggTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCt                                                                                                                                           >  4:184174/1‑149 (MQ=255)
          tCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGGCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTcc                                                                                                                                         <  7:141176/149‑1 (MQ=255)
                  cTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAg                                                                                                                                 <  3:184174/149‑1 (MQ=255)
                       ggATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGa                                                                                                                            <  1:94197/149‑1 (MQ=255)
                         aTCATGCCGTGCTTCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGaaa                                                                                                                          <  5:303467/149‑1 (MQ=255)
                             tGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACa                                                                                                                      <  8:62941/149‑1 (MQ=255)
                             tGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACa                                                                                                                      <  7:232097/149‑1 (MQ=255)
                             tGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACa                                                                                                                      >  3:9830/1‑149 (MQ=255)
                                   gctgcAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCATCCTTGACATGGGTGCGATcctgat                                                                                                                                          >  3:315645/1‑118 (MQ=38)
                                   gctgcAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCATCCTTGACATGGGTGCGATcctgat                                                                                                                                          <  4:315645/123‑6 (MQ=38)
                                    ctgcAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTgg                                                                                                                <  8:297518/148‑1 (MQ=255)
                                        aaCCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATc                                                                                                           >  2:184954/1‑149 (MQ=255)
                                        aaCCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATc                                                                                                           >  8:111744/1‑149 (MQ=255)
                                           cACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTAc                                                                                                        >  2:238666/1‑149 (MQ=255)
                                            aCTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGTGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTAc                                                                                                        >  2:17486/1‑148 (MQ=255)
                                                 ccccGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGTCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGc                                                                                                  <  7:111744/149‑1 (MQ=255)
                                                          cGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGc                                                                                         <  2:162770/149‑1 (MQ=255)
                                                               cTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGcc                                                                                    >  3:100008/1‑149 (MQ=255)
                                                                    cGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTg                                                                                              <  8:302105/134‑1 (MQ=255)
                                                                    cGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTg                                                                                              >  7:302105/1‑134 (MQ=255)
                                                                                 aaCGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGa                                                                                                                            <  3:146101/91‑1 (MQ=255)
                                                                                 aaCGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGa                                                                                                                            >  4:146101/1‑91 (MQ=255)
                                                                                 aaCGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCg                                                                  >  1:176993/1‑149 (MQ=255)
                                                                                    gggCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGttt                                                               >  8:175439/1‑149 (MQ=255)
                                                                                     ggCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTg                                                              <  4:9830/149‑1 (MQ=255)
                                                                                      gCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTgg                                                             >  3:221968/1‑149 (MQ=255)
                                                                                                   cgcCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCt                                                <  4:221968/149‑1 (MQ=255)
                                                                                                      ccGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTcgagtgag                                             >  1:115047/1‑142 (MQ=255)
                                                                                                          aCAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCTGAGCGAc                                         >  1:280412/1‑149 (MQ=255)
                                                                                                                aaTGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCTGAGCGACCCTCTg                                   <  7:175439/149‑1 (MQ=255)
                                                                                                                aaTGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCTGAGCGACCCTCTg                                   <  2:176993/149‑1 (MQ=255)
                                                                                                                     gTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCTGAGCGACCCTCTGCattg                              >  7:45232/1‑146 (MQ=255)
                                                                                                                               aTCGCATCCTTGACATGGGTGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCTGAGCGACCCTCTGCAAGTCGGGCCAgg                     >  2:305717/1‑148 (MQ=255)
                                                                                                                                    acacATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACAtg                                                                       >  5:244724/4‑93 (MQ=255)
                                                                                                                                    aCCCATGTCAAGTATTCGATCGCCTCCACCCACAGCTTGAATTACAGGCTGTCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACAtg                                                                       <  6:244724/93‑1 (MQ=255)
                                                                                                                                            cAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCTGAGCGACCCTCTGCAAGTCGGGCCAGGCAAGACGCTGGAAg       >  8:218063/1‑149 (MQ=255)
                                                                                                                                               ggATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCTGAGCGACCCTCTGCAAGTCGGGCCAGGCAAGACGCTGGAAGTGc    <  7:218063/149‑1 (MQ=255)
                                                                                                                                                gATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCTGAGCGACCCTCTGCAAGTCGGGCCAGGCAAGACGCTGGAAGTg     >  5:79207/1‑147 (MQ=255)
                                                                                                                                                 aTGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCTGAGCGACCCTCTGCAAGTCGGGCCAGGCAAGACGCTGGAAGTGCAg  <  2:280412/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTCAAGGATGCGATCGCCTCCACCCACAGCTTGAAATACAGCCTGGCGATCTACCAGAGCGGTGCACGCCTGCCGAAACTGGACATGCTGCGTTTGGCGATCGTTCCCCTGAGCGACCCTCTGCAAGTCGGGCCAGGCAAGACGCTGGAAGTGCAG  >  NC_002947/3762950‑3763243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑