Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,833,890 G→A 9.6% L185L (CTG→CTA)  vgrG‑II → type VI secretion system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,833,8900GA 9.6% 141.9 / 4.8 53L185L (CTG→CTA) vgrG‑IItype VI secretion system protein
Reads supporting (aligned to +/- strand):  ref base G (22/25);  new base A (3/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-01

CCCGCATCAGGTGCGCGAATTTTGCGTGCAGGCCGGTGAAACAGACCTCGACTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGTGCTGTACCGATCCGTTGGAGGCGGCGATCAGGCCAGGCCTTGCCTGCGGCGCTTGCG  >  NC_002947/3833749‑3834036
                                                                                                                                              |                                                                                                                                                  
cccGCATCAGGTGCGCGAATTTTGCGTGCAGGCCGGTGAAACAGACCTCGACTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGgcgc                                                                                                                                             >  6:85451/1‑149 (MQ=255)
 ccGCATCAGGTGCGCGAATTTTGCGTGCAGGCCGGTGAAACAGACCTCGACTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTCCTGGCGCt                                                                                                                                            >  6:255248/1‑149 (MQ=255)
     aTCAGGTGCGCGAATTTTGCGTGCAGGCCGGTGAAACAGACCTCGACTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGc                                                                                                                                        <  7:55608/149‑1 (MQ=255)
     aTCAGGTGCGCGAATTTTGCGTGCAGGCCGGTGAAACAGACCTCGACTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGc                                                                                                                                        <  2:4232/149‑1 (MQ=255)
         ggTGCGCGAATTTTGCGTGCAGGCCGGTGAAACAGACCTCGACTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGa                                                                                                                                    <  5:45512/149‑1 (MQ=255)
              gcgAATTTTGCGTGCAGGCCGGTGAAACAGACCTCGACTTCATGTCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGc                                                                                                                               >  7:25997/1‑149 (MQ=255)
              gcgAATTTTGCGTGCAGGCCGGTGAAACAGACCTCGACTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCAAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGc                                                                                                                               >  3:10936/1‑149 (MQ=255)
                  aTTTTGCGTGCAGGCCGGTGAAACAGACCTCGACTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCggg                                                                                                                           >  5:53929/1‑149 (MQ=255)
                     ttGCGTGCAGGCCGGTGAAACAGACCTCGACTTCATGTCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCggggg                                                                                                                         <  8:25997/148‑1 (MQ=255)
                         gTGCAGGCCGGTGAAACAGACCTCGACTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCAt                                                                                                                    >  1:203991/1‑149 (MQ=255)
                                        aCAGACCTCGACTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGAtgatga                                                                                                     <  8:109031/149‑1 (MQ=255)
                                         cAGACCTCGACTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAg                                                                                                    <  2:212503/149‑1 (MQ=255)
                                          aGACCTCGACTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTgg                                                                                                                                          <  7:220276/110‑1 (MQ=255)
                                          aGACCTCGACTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTgg                                                                                                                                          >  8:220276/1‑110 (MQ=255)
                                          aGACCTCGACTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAg                                                                                                    >  7:37975/1‑148 (MQ=255)
                                              cTCGACTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCTGTCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGAt                                                                                                 >  1:78261/1‑148 (MQ=255)
                                              cTCGACTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATg                                                                                                >  4:181174/1‑148 (MQ=255)
                                              cTCGACTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGa                                                                                               <  6:220808/149‑1 (MQ=255)
                                                 gACTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGaa                                                                                              <  1:170055/147‑1 (MQ=255)
                                                   cTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGc                                                                                                                                        >  6:55799/1‑103 (MQ=255)
                                                   cTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGc                                                                                                                                        <  5:55799/103‑1 (MQ=255)
                                                    ttCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCgggg                                                                                                                          <  8:162550/116‑1 (MQ=255)
                                                    ttCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCgggg                                                                                                                          >  7:162550/1‑116 (MQ=255)
                                                       aTGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAgg                                                                                            <  5:48511/143‑1 (MQ=255)
                                                       aTGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAgg                                                                                            >  6:48511/1‑143 (MQ=255)
                                                          gCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCTACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGTCATCAAGACCGATGATGAGGATGAAGGGTTTcaca                                                                                    <  5:86983/148‑1 (MQ=255)
                                                              gCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTACTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATgac                                                                               >  6:281218/1‑149 (MQ=255)
                                                              gCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTACTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATgac                                                                               >  6:286386/1‑149 (MQ=255)
                                                                     cgccgAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTgg                                                                        <  7:10024/149‑1 (MQ=255)
                                                                              aGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGAt                                                                >  1:258971/1‑148 (MQ=255)
                                                                              aGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATc                                                               <  2:258971/149‑1 (MQ=255)
                                                                                  aTTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTACTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGt                                                           <  5:281218/149‑1 (MQ=255)
                                                                                  aTTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTACTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGt                                                           <  5:286386/149‑1 (MQ=255)
                                                                                     gTCTTCCGCT‑TCGACCACACGCCGAAACTGCACTTGCTAATCATCTCTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGTGc                                                         <  5:255248/148‑1 (MQ=255)
                                                                                      tCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGTGCt                                                        >  8:308588/1‑148 (MQ=255)
                                                                                      tCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGTGCt                                                        <  7:308588/148‑1 (MQ=255)
                                                                                          ccGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGTGCTGt                                                      <  6:53929/146‑1 (MQ=255)
                                                                                             cT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGTGCTGTACCGAt                                                >  1:72618/1‑149 (MQ=255)
                                                                                               ttctaacacaCGCCGCAACAGCACTTGGTAATCATCCCTGACCGGCTGCTGGCGCGGGGCCAGATCAGGCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGTGCTGTACCGATc                                               <  4:10936/143‑1 (MQ=255)
                                                                                                 cGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGTGCTGTACCGATcc                                              <  2:87937/148‑1 (MQ=255)
                                                                                                   aCCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGTGCTGTACCGATCCGtt                                           <  5:85451/149‑1 (MQ=255)
                                                                                                      acacGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCgatgat                                                                                                       <  2:330862/86‑1 (MQ=255)
                                                                                                      acacGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCgatgat                                                                                                       >  1:330862/1‑86 (MQ=255)
                                                                                                      acacGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGTGCTGTACCGATCCGTTg                                          >  8:67481/1‑147 (MQ=255)
                                                                                                       cacGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGTGCTGTACCGATCCGTTGGAg                                       <  2:203991/149‑1 (MQ=255)
                                                                                                          gCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCCATCAGGTGCTGTACCGATCCGTTTGAggcg                                    >  7:270715/1‑149 (MQ=255)
                                                                                                                                     tGACCGGCTGCTGTCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGTGCTGTACCGATCCGTTGGAGGCGGCGATCAGGCCAGGCCTTGCCTgcggc         >  6:239860/1‑149 (MQ=255)
                                                                                                                                     tGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGTGCTGTACCGATCCGTTGGAGGCGGCGATCAGGCCAGGCCTTGCCTgcggc         >  7:77968/1‑149 (MQ=255)
                                                                                                                                       aCCGGCTGCTGTCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGTGCTGTACCGATCCGTTGGAGGCGGCGATCAGGCCAGGCCTTGCCTgcggc         <  5:239860/147‑1 (MQ=255)
                                                                                                                                       aCCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGTGCTGTACCGATCCGTTGGAGGCGGCGATCAGGCCAGGCCTTGCCTgcggc         <  3:181174/147‑1 (MQ=255)
                                                                                                                                         cGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGTGCTGTACCGATCCGTTGGAGGCGGCGATCAGGCCAGGCCTTGCCTGCGGCGCtt     >  8:152579/1‑149 (MQ=255)
                                                                                                                                         cGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGTGCTGTACCGATCCGTTGGAGGCGGCGATCAGGCCAGGCCTTGCCTGCGGCGCtt     >  2:302883/1‑149 (MQ=255)
                                                                                                                                         cGGCTACTGGCGCTGGGCCAGATCAGCCGGGGGGCCAACAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGTGCTGTACCGATCCGTTGGAGGCGGCGATCAGGCCAGGCCTTGCCTGCGGCGCtt     >  2:43629/1‑149 (MQ=255)
                                                                                                                                            ctgctgGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGTGCTGTACCGATCCGTTGGAGGCGGCGATCAGGCCAGGCCTTGCCTGCGGCGCTTGCg  >  5:32255/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
CCCGCATCAGGTGCGCGAATTTTGCGTGCAGGCCGGTGAAACAGACCTCGACTTCATGGCCCGCCTGGCCGCCGAGGAAGGCATTGTCTACCGCT‑TCGACCACACGCCGAAACAGCACTTGCTAATCATCACTGACCGGCTGCTGGCGCTGGGCCAGATCAGCCGGGGGGCCATCAAGACCGATGATGAGGATGAAGGGTTTCACAATGACGACGTGGGCCCCGATCAGGTGCTGTACCGATCCGTTGGAGGCGGCGATCAGGCCAGGCCTTGCCTGCGGCGCTTGCG  >  NC_002947/3833749‑3834036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑