Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,854,437 G→A 100% P256S (CCG→TCG)  PP_3403 ← quercetin 2,3‑dioxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,854,4370GA99.8% 144.7 / ‑7.3 45P256S (CCG→TCG) PP_3403quercetin 2,3‑dioxygenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (24/20);  minor base C (1/0);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGGCCAACCAATTGCATCAGTGTGCGGCGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCGGTCGAGCAGTACCAGGCTGGACGGGCCTGCTTCGCGCTCGCCGTTGATTCGAACGTTACCACGCAAC  >  NC_002947/3854299‑3854582
                                                                                                                                          |                                                                                                                                                 
aGGCCAACCAATTGCATCAGTGTGCGGCGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATc                                                                                                                                         <  3:294371/149‑1 (MQ=255)
   ccaaccaaTTGCATCAGTGTGCGGCGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGc                                                                                                                                      >  2:186187/1‑149 (MQ=255)
   caaaccaaTTGCATCAGTGTGCGGCGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGc                                                                                                                                      <  8:211181/147‑1 (MQ=255)
    caaccaaTTGCATCAGTGTGCGGCGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACGAGTCGATCgttg                                                                                                                                     <  3:319138/149‑4 (MQ=255)
    caaccaaTTGCATCAGTGTGAGGCGCCTCCCCTCTCGTCCTGCATCAGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTACGCAATCTCAGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGc                                                                                                                                      >  5:53784/1‑148 (MQ=255)
         aaTTGCATCAGTGTGCGGCGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTAGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCg                                                                                                                                >  7:70071/1‑149 (MQ=255)
                              cTCCCCTCTCGTCCTGCATCTGCCAAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTgcg                                                                                                           <  6:228590/149‑1 (MQ=255)
                                ccccTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGCCTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGcc                                                                                                         >  2:310433/1‑149 (MQ=255)
                                        gTCCTTCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAg                                                                                                   <  7:98776/147‑1 (MQ=255)
                                           cTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCt                                                                                              >  2:56668/1‑149 (MQ=255)
                                               aTCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGAc                                                                                          <  4:119177/149‑1 (MQ=255)
                                                     ccAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTa                                                                                     >  5:310665/1‑148 (MQ=255)
                                                     ccAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTa                                                                                     >  5:219684/1‑148 (MQ=255)
                                                      cAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTAc                                                                                    >  5:82729/1‑148 (MQ=255)
                                                         aCCGCCCGGCATGGATATCATCGAACGAGTCGGCAATCTCCGCCTGGCGGTTCATCACGAAGGGGCCGTAGCCAACGATCGACTCGTCTATCGGCTCACCGCTTAGCAGGAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTAGAtgt                                                                                <  6:53784/149‑3 (MQ=255)
                                                            gCCCGGCATGGAAATCATCGAACGACTCGGCAAACTCCGCCTGGCTGTTAATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTACGCCGTCCAGCGCCTCGACGCCTCCATCTaca                                                                             >  5:94493/1‑149 (MQ=255)
                                                                 gCATGGAAATCATCGAGCGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTc                                                                        >  6:74575/1‑149 (MQ=255)
                                                                 gCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTc                                                                        <  6:310665/149‑1 (MQ=255)
                                                                     ggAAAGCATCGAACGACTCGCCAATCTCCGCCTGGCTGTTCATCACCAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGTTGTTTTCATAGGCTCTACTCCTAGATCTACACCCTCCCgg                                                                    <  4:297753/149‑1 (MQ=255)
                                                                      gAAATCATCGAGCGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCGGt                                                                   <  5:74575/149‑1 (MQ=255)
                                                                       aaaTCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTgc                                                                                                            <  8:46410/107‑1 (MQ=255)
                                                                       aaaTCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTgc                                                                                                            >  7:46410/1‑107 (MQ=255)
                                                                          tcatcGAACGCCTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCGGTCGAg                                                               >  7:55925/1‑149 (MQ=255)
                                                                            atcGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCTAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTac                                                                              <  7:254399/132‑1 (MQ=255)
                                                                            atcGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCTAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTac                                                                              >  8:254399/1‑132 (MQ=255)
                                                                                 aCGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCGGTCGAGCAGTAcc                                                        <  6:219684/149‑1 (MQ=255)
                                                                                  cGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACTTCTACACCCTCCCGGTCGAGCAGTACCa                                                       <  1:186187/149‑1 (MQ=255)
                                                                                      tCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCGGTCGAGCAGTACCAGGCt                                                   >  3:67921/1‑149 (MQ=255)
                                                                                        ggCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCGGTCGAGCAGTACCAGGCTg                                                  <  7:19260/148‑1 (MQ=255)
                                                                                           aaTCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGTTCGACTCGTCGATCGGCTCACCGCTGAGCAGCGGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCGGTCGAGCAGTACCAGGCTGGACg                                              >  1:228943/1‑149 (MQ=255)
                                                                                            aTCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCGGTCGAGCAGTACCAGGCTGGACgg                                             <  1:56668/149‑1 (MQ=255)
                                                                                               tcCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCGGTCGAGCAGTACCAGGCTGGACGGGcc                                          >  5:98867/1‑149 (MQ=255)
                                                                                                       gCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCGGTCGAGCAGTACCAGGCTGGACGGGCCTGCTTcgc                                  >  4:35155/1‑149 (MQ=255)
                                                                                                       gCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCGGTCGAGCAGTACCAGGCTGGACGGGCCTGCTTcgc                                  >  6:45329/1‑149 (MQ=255)
                                                                                                       gCTGTTCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCGGTCGAGCAGTACCAGGCTGGACGGGCCTGCTTc                                    <  4:67921/147‑1 (MQ=255)
                                                                                                           ttCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGcc                                                                                       >  4:44609/1‑92 (MQ=255)
                                                                                                           ttCATCACGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGcc                                                                                       <  3:44609/92‑1 (MQ=255)
                                                                                                               tcaCGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCGGTCGAGCAGTACCAGGCTGGACGGGCCTGCTTCGCGCTCGCCg                          >  2:94237/1‑149 (MQ=255)
                                                                                                                 aCGAACGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCGGTCGAGCAGTACCAGGCTGGACGGGCCTGCTTCGCGCTCGCCGtt                        >  2:41660/1‑149 (MQ=255)
                                                                                                                     aCGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCGGTCGAGCAGTACCAGGCTGGACGGGCCTGCTTCGCGCTCGCCGTTGAt                     <  6:82729/148‑1 (MQ=255)
                                                                                                                      cGGGCCGTAGCCAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCGGTCGAGCAGTACCAGGCTGGACGGGCCTGCTTCGCGCTCGCCGTTGATTc                   <  6:98867/149‑1 (MQ=255)
                                                                                                                                ccAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCGGTCGAGCAGTACCAGGCTGGACGGGCCTGCTTCGCGCTCGCCGTTGATTCGAACGTTAcc         >  8:85977/1‑149 (MQ=255)
                                                                                                                                 cAACGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCGGTCGAGCAGTACCAGGCTGGACGGGCCTGCTTCGCGCTCGCCGTTGATTCGAACGTTACCa        >  1:190134/1‑149 (MQ=255)
                                                                                                                                  aaCGATCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCGGTCGAGCAGTACCAGGCTGGACGGGCCTGCTTCGCGCTCGCCGTTGATTCGAACGTTACCAc       >  7:149594/1‑149 (MQ=255)
                                                                                                                                       tCGACTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCCGTCGAGCAGTACCAGGCTGGACGGGCCTCCTTCGCGCACGCCGTAGATTCGAACGTTACCACGCAac  >  2:140033/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                 
AGGCCAACCAATTGCATCAGTGTGCGGCGCCTCCCCTCTCGTCCTGCATCTGCCCAAACCGCCCGGCATGGAAATCATCGAACGACTCGGCAATCTCCGCCTGGCTGTTCATCACGAACGGGCCGTAGCCAACGATCGGCTCGTCGATCGGCTCACCGCTGAGCAGCAGCACACTTGCGCCGTCCAGCGCCTCGACGCCTACATCTACACCCTCCCGGTCGAGCAGTACCAGGCTGGACGGGCCTGCTTCGCGCTCGCCGTTGATTCGAACGTTACCACGCAAC  >  NC_002947/3854299‑3854582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑