Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,880,375 C→G 7.1% A737G (GCC→GGC)  mexF → multidrug RND transporter MexF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,880,3750CG 7.1% 175.8 / 4.2 56A737G (GCC→GGC) mexFmultidrug RND transporter MexF
Reads supporting (aligned to +/- strand):  ref base C (26/25);  new base G (2/2);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.39e-01

GGTTCCGTCTGCAAATCGAAGACCGTGGCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCACTTACCAGGTCAACGTCCAGGCCGAGCAGC  >  NC_002947/3880228‑3880521
                                                                                                                                                   |                                                                                                                                                  
ggTTCCGTCTGCAAATCGAAGACCGTGGCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATgcc                                                                                                                                                   <  4:194328/149‑1 (MQ=255)
     cGTCTGCAAATCGAAGACCGTGGCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCAt                                                                                                                                              >  6:147955/1‑149 (MQ=255)
     cGTCTGCAAATCGAAGACCGTGGCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCAt                                                                                                                                              <  4:229199/149‑1 (MQ=255)
      gTCTGCAAATCGAAGACCGTGGCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCAt                                                                                                                                              <  6:71053/148‑1 (MQ=255)
      gTCTGCAAATCGAAGACCGTGGCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCAt                                                                                                                                              >  5:71053/1‑148 (MQ=255)
             aaTCGTAGACCGTGTCAACCTGTGTTACGAAGCGCGGGACAAGGAAACCCAGATCATCATCGCCAAGCGCCACAACGTGCCAGCGCTGGCACGCCTGTTCACCAGCTACCAGGTCAACGTGCCTCACGTCGATGGCGTCATCGActtgt                                                                                                                                      <  4:26697/149‑5 (MQ=255)
                        gTGGCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAg                                                                                                                           <  1:146149/149‑1 (MQ=255)
                          ggCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCaa                                                                                                                            >  4:167574/1‑146 (MQ=255)
                          ggCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCaa                                                                                                                            <  7:150815/146‑1 (MQ=255)
                          ggCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCaa                                                                                                                            <  3:167574/146‑1 (MQ=255)
                          ggCAACCTGGGTTACGAAGCGCTGTACAAGCAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCaa                                                                                                                            >  8:150815/1‑146 (MQ=255)
                                cTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACgg                                                                                                                   >  1:284095/1‑149 (MQ=255)
                                       aCGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTgg                                                                                                              >  8:213062/1‑147 (MQ=255)
                                       aCGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTgg                                                                                                              <  7:213062/147‑1 (MQ=255)
                                        cGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCg                                                                                                            <  2:284095/148‑1 (MQ=255)
                                         gAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGAt                                                                                                          >  3:63519/1‑149 (MQ=255)
                                             cgcTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCAcc                                                                                                      >  4:92687/1‑149 (MQ=255)
                                              gcTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAGCGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGGCGGCATCGActtgt                                                                                                                                      >  2:118278/1‑112 (MQ=255)
                                              gcTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAGCGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGGCGGCATCGActtgt                                                                                                                                      <  1:65202/116‑5 (MQ=255)
                                              gcTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAGCGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGGCGGCATCGActtgt                                                                                                                                      >  2:65202/1‑112 (MQ=255)
                                                  tACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGAc                                                                                                   <  3:92687/147‑1 (MQ=255)
                                                     aaGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCtt                                                                                              >  1:51715/1‑149 (MQ=255)
                                                        gAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGa                                                                                           >  3:80862/1‑149 (MQ=255)
                                                             ccAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGCCCGGGAAAAGGCCAAGACCCACGCTGTGGCGATCCCCGACATCTTCGACAccc                                                                                      >  7:270593/1‑149 (MQ=255)
                                                             ccAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACAt                                                                                                 <  5:237059/138‑1 (MQ=255)
                                                             ccAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACAt                                                                                                 >  6:237059/1‑138 (MQ=255)
                                                                  aCATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAATGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCa                                                                                  <  7:69075/148‑1 (MQ=255)
                                                                  aCATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAg                                                                                 <  4:80862/149‑1 (MQ=255)
                                                                      cttcGGCATGAGCCACAACGTCCCTGTGCTGGCTGGCGTGTTCACCAGCAACCAGGGCAAGGTGCCACAAGTCGATGCCGCCATCGAGCGGGAAAAGGCCAAGACCCTCGGtgtg                                                                                                               <  1:248015/113‑1 (MQ=255)
                                                                      catcGCCAACAGCCACAACGTCCCAGAGCTGCCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAACTCGATCACGCCATCGACCGGGAAAAGGCCAAGACCCACGGtgtg                                                                                                               >  2:248015/1‑115 (MQ=255)
                                                                        tcGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTAc                                                                           <  4:63519/149‑1 (MQ=255)
                                                                          gCCACGTGCCACCACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTAc                                                                           <  8:270593/147‑1 (MQ=255)
                                                                          gCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCt                                                                         <  1:67563/149‑1 (MQ=255)
                                                                               gagCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCt                                                                    >  2:184739/1‑149 (MQ=255)
                                                                                  ccACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGc                                                                 <  5:147955/149‑1 (MQ=255)
                                                                                     caACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTg                                                               >  4:271506/1‑148 (MQ=255)
                                                                                       aCGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTac                                                            <  2:51715/149‑1 (MQ=255)
                                                                                             cAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAAc                                                      <  4:162429/149‑1 (MQ=255)
                                                                                             cAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAAc                                                      >  3:162429/1‑149 (MQ=255)
                                                                                                         gCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGc                                          >  4:182475/1‑149 (MQ=255)
                                                                                                          ccTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCAc                                                                                                       >  8:170213/1‑87 (MQ=255)
                                                                                                          ccTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCAc                                                                                                       <  7:170213/87‑1 (MQ=255)
                                                                                                           cTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCt                                                                         <  2:167376/116‑1 (MQ=255)
                                                                                                           cTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCt                                                                         >  1:167376/1‑116 (MQ=255)
                                                                                                                 aCCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTgg                                     <  8:107874/146‑1 (MQ=255)
                                                                                                                 aCCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTgg                                     >  7:107874/1‑146 (MQ=255)
                                                                                                                   cAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCa                                >  1:321873/1‑149 (MQ=255)
                                                                                                                   cAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCa                                >  6:280624/1‑149 (MQ=255)
                                                                                                                         ccAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCACTTAc                           >  2:186905/1‑148 (MQ=255)
                                                                                                                          cAGGTCAACGTGCCACAAGTCGATGCCGCCATCAACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCACTTAc                           <  2:115681/147‑1 (MQ=255)
                                                                                                                          cAGGTCAACGTGCCACAAGTCGATGCCGCCATCAACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCACTTAc                           >  1:115681/1‑147 (MQ=255)
                                                                                                                                       cacaAGTCGATGCCCCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCACTTACCAGGTCAACGTCCAg            >  7:277254/1‑149 (MQ=255)
                                                                                                                                        acaAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCACTTACCAGGTCAACGTCCAgg           >  6:145635/1‑149 (MQ=255)
                                                                                                                                             tCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCACTTACCAGGTCAACGTCCAGGCCGag      >  3:108707/1‑149 (MQ=255)
                                                                                                                                             tCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCACTTACCAGGTCAACGTCCAGGCCGag      <  3:182475/149‑1 (MQ=255)
                                                                                                                                                 tGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCACTTACCAGGTCAACGTCCAGGCCGagcagc  <  1:184739/149‑1 (MQ=255)
                                                                                                                                                 tGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCACTTACCAGGTCAACGTCCAGGCCGagcagc  <  3:271506/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGTTCCGTCTGCAAATCGAAGACCGTGGCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCACTTACCAGGTCAACGTCCAGGCCGAGCAGC  >  NC_002947/3880228‑3880521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑