Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,931,916 T→C 100% I521V (ATC→GTC)  PP_3466 ← ABC transporter permease/ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,931,9160TC100.0% 132.1 / ‑6.8 41I521V (ATC→GTC) PP_3466ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (17/23);  minor base G (0/1);  total (17/24)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CATGTTTGATCACCTCCTGCGCCAGTACCTGCAACTGGCCGTCATGCAGTTGCAGCTGCAGGTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGATCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGC  >  NC_002947/3931775‑3932059
                                                                                                                                             |                                                                                                                                               
cATGTTTGATCACCTCCTGCGCCAGTACCTGCAACTGGCCGTCATGCAGTTGCAGCTGCAGGTCGGCATCGGCCAGGGTCGAAGACCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCa                                                                                                                                          >  7:22628/1‑149 (MQ=255)
cATGTTTGATCACCTCCTGCGCCAGTACCTGCAACTGGCCGTCATGCAGTTGCAGCTGCAGGTCGGCATCGGCCACGGTCGAAGCCCGGTGCCTGATCAGGATGCGCGTGCGGCCGCCGAACAGCTGGTCCATGGCCGCGACCACTTCa                                                                                                                                          >  3:178028/1‑149 (MQ=255)
    tttGATCACCTCCTGCGCCAGTACCTGCAACTGGCCGTCATGCAGTTGCAGCTGCAGGTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCt                                                                                                                                      >  3:112742/1‑149 (MQ=255)
           aCCTCCTGCGCCAGTACCTGCAACTGGCCGTCATGCAGTTGCAGCTGCAGGTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTgg                                                                                                                                <  5:193953/148‑1 (MQ=255)
            cctcctGCGCCAGTACCTGCAACTGGCCGTCATGCAGTTGCAGCTGCAGGTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGcc                                                                                                                              <  3:287269/149‑1 (MQ=255)
             ctcctGCGCCAGTACCTGCAACTGGCCGTCATGCAGTTGCAGCTGCAGGTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCt                                                                                                                             >  1:74660/1‑149 (MQ=255)
              tcctGCGCCAGTACCTGCAACTGGCCGTCATGCAGTTGCAGCTGCAGGTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCt                                                                                                                             >  1:282451/1‑148 (MQ=255)
                        gTACCTGCAACTGGGCGTCATGCAGTTGCTGCTGCAGGTGGCCATCGTCCAGGGTAGAAGCGCGGTGGCTGATCAGGATGCGCGGGCGTCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCAGTGcc                                                                                                                  <  4:178028/149‑1 (MQ=255)
                         tACCTGCAACTGGCCGTCATGCAGTTGCAGCTGCAGGTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGcc                                                                                                                  >  2:142664/1‑148 (MQ=255)
                              gCAACTGGCCGTCATGCAGTTGCAGCTGCAGGTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTg                                                                                                            >  7:54178/1‑149 (MQ=255)
                               cAACTGGCCGTCATGCAGTTGCAGCTGCAGGTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGt                                                                                                             <  2:282451/147‑1 (MQ=255)
                               cAACTGGCCGTCATGCAGTTGCAGCTGCAGGTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTg                                                                                                            <  8:155350/148‑1 (MQ=255)
                                     gCCGTCATGCAGTTGCAGCTGCAGGTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTc                                                                                                       <  2:195564/147‑1 (MQ=255)
                                       cGTCATGCAGTTGCAGCTACAGGTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGt                                                                                                     <  3:226714/147‑1 (MQ=255)
                                       cGTCATGCAGTTGCAGCTACAGGTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGt                                                                                                     >  4:226714/1‑147 (MQ=255)
                                         tCATGCAGTTGCAGCTGCAGGTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCg                                                                                                                                    <  2:320792/114‑1 (MQ=255)
                                         tCATGCAGTTGCAGCTGCAGGTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCg                                                                                                                                    >  1:320792/1‑114 (MQ=255)
                                                  tGCAGCTGCAGGTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGAt                                                                                        >  4:248198/1‑149 (MQ=255)
                                                     aGCTGCAGGTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGc                                                                                     >  2:239663/1‑149 (MQ=255)
                                                     aGCTGCAGGTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGc                                                                                     <  4:75888/149‑1 (MQ=255)
                                                     aGCTGCAGGTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGc                                                                                     <  3:167295/149‑1 (MQ=255)
                                                             gTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCt                                                                             <  1:239663/149‑1 (MQ=255)
                                                                ggCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGc                                                                           <  1:272397/148‑1 (MQ=255)
                                                                       gCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGc                                                                   >  8:19247/1‑149 (MQ=255)
                                                                        ccAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCa                                                                  <  4:112742/149‑1 (MQ=255)
                                                                           gggTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGa                                                               <  8:54178/149‑1 (MQ=255)
                                                                                   gCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGc                                                       <  7:19247/149‑1 (MQ=255)
                                                                                             tGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCgttgt                                             >  7:279433/1‑149 (MQ=255)
                                                                                                  aGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCt                                         <  2:74660/148‑1 (MQ=255)
                                                                                                  aGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCt                                         <  6:155562/148‑1 (MQ=255)
                                                                                                  aGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTg                                        <  1:142664/149‑1 (MQ=255)
                                                                                                               ggccggcGAACAGCTGGTCGATGGCCGCGACCACTTAACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCACGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACaa                            >  2:303835/1‑148 (MQ=255)
                                                                                                                     cGAACAGCTGGGCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTgg                     <  3:248198/149‑1 (MQ=255)
                                                                                                                         cAGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCcgc                 >  5:156090/1‑149 (MQ=255)
                                                                                                                          aGCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTACTGGCCCCCGGACAACTGCTGGGcgag                >  3:200436/1‑147 (MQ=255)
                                                                                                                           gCTGGTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCgcgc               <  1:303835/149‑1 (MQ=255)
                                                                                                                              ggTCGATGGCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGGCGAGcgcg            >  6:224456/1‑149 (MQ=255)
                                                                                                                                      gCCGCGAGGACTTCACGGTCGGTTGCCTCATCCTCTGCCGAAGGGGCCTCGTCCTGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCATTGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGGCGAGCGCGGCCAGCag    <  4:200436/149‑2 (MQ=255)
                                                                                                                                      gCCGCGACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCaa    <  8:279433/149‑1 (MQ=255)
                                                                                                                                        cgcgACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGGCGAGCGCGGCCAGCagtt  <  5:224456/149‑4 (MQ=255)
                                                                                                                                        cgcgACCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGc  <  6:156090/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                               
CATGTTTGATCACCTCCTGCGCCAGTACCTGCAACTGGCCGTCATGCAGTTGCAGCTGCAGGTCGGCATCGGCCAGGGTCGAAGCCCGGTGGCTGATCAGGATGCGCGTGCGGCCGGCGAACAGCTGGTCGATGGCCGCGATCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGC  >  NC_002947/3931775‑3932059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑