Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,947,128 A→G 6.5% G307G (GGT→GGC) ‡ PP_3483 ← type II secretion system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,947,1280AG 6.5% 171.8 / 2.5 61G307G (GGT→GGC) ‡PP_3483type II secretion system protein
Reads supporting (aligned to +/- strand):  ref base A (29/28);  new base G (2/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.65e-01

CGGTGACCAGGATCATGCCGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATCACCTGGTCGGCGAA  >  NC_002947/3946980‑3947274
                                                                                                                                                    |                                                                                                                                                  
cGGTGACCAGGATCATGCCGTAGGGGTCTGCCTCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCa                                                                                                                                                    <  5:20624/149‑1 (MQ=255)
    gACCAGGATCATGCCGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCg                                                                                                                                                 >  4:260639/1‑148 (MQ=255)
       cAGGATCATGCCGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCg                                                                                                                                                 >  4:187994/1‑145 (MQ=255)
           aTCATGCCGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACAGCATCTTCGGTGAAGATGcc                                                                                                                                         <  8:118155/149‑2 (MQ=255)
            tCATGCCGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGTTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTa                                                                                                                                        <  1:89431/149‑1 (MQ=255)
            tCATGCCGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCACTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCGGTGAAGATGccg                                                                                                                                        <  4:14630/149‑3 (MQ=255)
             cATGCCGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAg                                                                                                                                       >  1:209023/1‑149 (MQ=255)
              aTGCCGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAg                                                                                                                                       <  3:260639/148‑1 (MQ=255)
              aTGCCGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAg                                                                                                                                       <  3:187994/148‑1 (MQ=255)
                  cGTTGGGTTCGTCGGCCAACCTGCGCAGGGCGAGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATg                                                                                                                                  <  6:229179/149‑1 (MQ=255)
                  cGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCAt                                                                                                                                   >  5:65483/1‑148 (MQ=255)
                  cGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCAt                                                                                                                                   >  1:223947/1‑148 (MQ=255)
                      gggTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATc                                                                                                                               <  1:12708/148‑1 (MQ=255)
                        gTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGac                                                                                                                            >  4:110199/1‑149 (MQ=255)
                        gTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGa                                                                                                                             >  4:105472/1‑148 (MQ=255)
                                    aCCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTc                                                                                                                 >  2:115835/1‑148 (MQ=255)
                                      cGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGAt                                                                                                              >  5:272853/1‑149 (MQ=255)
                                          gcAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTa                                                                                                                                        <  4:276946/119‑1 (MQ=255)
                                          gcAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTa                                                                                                                                        >  3:276946/1‑119 (MQ=255)
                                            aGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCg                                                                                                        >  5:220499/1‑149 (MQ=255)
                                                    cAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACAccc                                                                                                                        <  4:18082/125‑1 (MQ=255)
                                                    cAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACAccc                                                                                                                        >  3:18082/1‑125 (MQ=255)
                                                     aaGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCAc                                                                                               >  2:200725/1‑149 (MQ=255)
                                                     aaGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCAc                                                                                               >  7:201785/1‑149 (MQ=255)
                                                        gTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGc                                                                                            <  4:170746/149‑1 (MQ=255)
                                                         tttCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGcc                                                                                           >  8:85100/1‑149 (MQ=255)
                                                                ggCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTg                                                                                     <  8:201785/148‑1 (MQ=255)
                                                                   aaaGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGATCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGc                                                                                 <  2:275707/149‑1 (MQ=255)
                                                                       cccAAGGCTTGCAGCTGCACGCAGCTGACCAGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGcc                                                                             <  4:13777/149‑1 (MQ=255)
                                                                        ccAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCgg                                                                               >  1:17969/1‑146 (MQ=255)
                                                                        ccAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCgg                                                                               <  2:17969/146‑1 (MQ=255)
                                                                        ccAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCg                                                                            >  3:203248/1‑149 (MQ=255)
                                                                          aaGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTc                                                                          >  1:156213/1‑149 (MQ=255)
                                                                           aGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTcc                                                                         <  6:220499/149‑1 (MQ=255)
                                                                             gCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTg                                                                       <  2:156213/149‑1 (MQ=255)
                                                                              cTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTgg                                                                      <  1:200725/149‑1 (MQ=255)
                                                                                    gCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCAcg                                                                >  6:204225/1‑149 (MQ=255)
                                                                                            ccgcTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTc                                                        <  3:75192/149‑1 (MQ=255)
                                                                                                  aCCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGAt                                                  <  7:85100/149‑1 (MQ=255)
                                                                                                    ccGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGt                                                >  1:14495/1‑149 (MQ=255)
                                                                                                      ggTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTcc                                              <  1:115835/149‑1 (MQ=255)
                                                                                                           gACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGc                                                      <  3:219487/136‑1 (MQ=255)
                                                                                                           gACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGc                                                      >  4:219487/1‑136 (MQ=255)
                                                                                                                gTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGATTTTCGACGATGTCCACCTCGGCCa                                    >  4:169093/1‑149 (MQ=255)
                                                                                                                       ttGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCtt                             >  7:280150/1‑149 (MQ=255)
                                                                                                                         gTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGa                           >  6:233993/1‑149 (MQ=255)
                                                                                                                         gTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGC‑AGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGAt                          >  1:67905/1‑149 (MQ=255)
                                                                                                                          tCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGAt                          <  3:123311/149‑1 (MQ=255)
                                                                                                                            gAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATgc                        >  6:147101/1‑149 (MQ=255)
                                                                                                                              gCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATgcgc                      <  5:147101/149‑1 (MQ=255)
                                                                                                                               cACCCGCAGCACGGCATCTTCACCGAAGATGC‑AGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCga                    <  2:67905/149‑1 (MQ=255)
                                                                                                                                  ccGCATCACGGCATCTTCACCGATGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCTGCCGTCCTGGGGCACGCGTTTTTCGcc                                                     <  5:298963/114‑1 (MQ=255)
                                                                                                                                  ccGCAGCACGGCATCTACACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCACACGGCCAACTTGAAGCGGCCGTCCTGGGGCACGCCTTTTTcccc                                                     >  6:298963/1‑114 (MQ=255)
                                                                                                                                    gcagcaCGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATc                <  4:203248/149‑1 (MQ=255)
                                                                                                                                     cagcaCGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATCa               >  4:121446/1‑149 (MQ=255)
                                                                                                                                       gcaCGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATCAcc             <  2:223947/149‑1 (MQ=255)
                                                                                                                                         ctagCATCTTCGGTGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATCACCTg           >  4:319847/4‑149 (MQ=255)
                                                                                                                                          tagCATCTTCGGTGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATCACCTgg          >  3:60054/3‑149 (MQ=255)
                                                                                                                                              aTCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATCACCTGGTCgg      >  6:154538/1‑149 (MQ=255)
                                                                                                                                                   cACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATCACCTGGTCGGCGaa  <  3:105472/148‑1 (MQ=255)
                                                                                                                                                   cACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATCACCTGGTCGGCGaa  <  3:110199/148‑1 (MQ=255)
                                                                                                                                                   cACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATCACCTGGTCGGCGa   <  2:14495/147‑1 (MQ=255)
                                                                                                                                                    aCCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCga                    <  4:276753/129‑1 (MQ=255)
                                                                                                                                                    aCCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCga                    >  3:276753/1‑129 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGGTGACCAGGATCATGCCGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATCACCTGGTCGGCGAA  >  NC_002947/3946980‑3947274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑