Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,947,129 C→G 6.4% G307A (GGT→GCT) ‡ PP_3483 ← type II secretion system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,947,1290CG 6.4% 199.5 / 4.2 63G307A (GGT→GCT) ‡PP_3483type II secretion system protein
Reads supporting (aligned to +/- strand):  ref base C (30/29);  new base G (2/2);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.44e-01

GACCAGGATCATGCCGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATCACCTGGTCGGCGAA  >  NC_002947/3946984‑3947274
                                                                                                                                                 |                                                                                                                                                 
gACCAGGATCATGCCGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCg                                                                                                                                                 >  4:260639/1‑148 (MQ=255)
   cAGGATCATGCCGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCg                                                                                                                                                 >  4:187994/1‑145 (MQ=255)
       aTCATGCCGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACAGCATCTTCGGTGAAGATGcc                                                                                                                                         <  8:118155/149‑2 (MQ=255)
        tCATGCCGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGTTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTa                                                                                                                                        <  1:89431/149‑1 (MQ=255)
        tCATGCCGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCACTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCGGTGAAGATGccg                                                                                                                                        <  4:14630/149‑3 (MQ=255)
         cATGCCGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAg                                                                                                                                       >  1:209023/1‑149 (MQ=255)
          aTGCCGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAg                                                                                                                                       <  3:260639/148‑1 (MQ=255)
          aTGCCGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAg                                                                                                                                       <  3:187994/148‑1 (MQ=255)
              cGTTGGGTTCGTCGGCCAACCTGCGCAGGGCGAGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATg                                                                                                                                  <  6:229179/149‑1 (MQ=255)
              cGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCAt                                                                                                                                   >  5:65483/1‑148 (MQ=255)
              cGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCAt                                                                                                                                   >  1:223947/1‑148 (MQ=255)
                  gggTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATc                                                                                                                               <  1:12708/148‑1 (MQ=255)
                    gTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGac                                                                                                                            >  4:110199/1‑149 (MQ=255)
                    gTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGa                                                                                                                             >  4:105472/1‑148 (MQ=255)
                                aCCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTc                                                                                                                 >  2:115835/1‑148 (MQ=255)
                                  cGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGAt                                                                                                              >  5:272853/1‑149 (MQ=255)
                                      gcAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTa                                                                                                                                        <  4:276946/119‑1 (MQ=255)
                                      gcAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTa                                                                                                                                        >  3:276946/1‑119 (MQ=255)
                                        aGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCg                                                                                                        >  5:220499/1‑149 (MQ=255)
                                                cAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACAccc                                                                                                                        <  4:18082/125‑1 (MQ=255)
                                                cAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACAccc                                                                                                                        >  3:18082/1‑125 (MQ=255)
                                                 aaGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCAc                                                                                               >  2:200725/1‑149 (MQ=255)
                                                 aaGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCAc                                                                                               >  7:201785/1‑149 (MQ=255)
                                                    gTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGc                                                                                            <  4:170746/149‑1 (MQ=255)
                                                     tttCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGcc                                                                                           >  8:85100/1‑149 (MQ=255)
                                                            ggCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTg                                                                                     <  8:201785/148‑1 (MQ=255)
                                                               aaaGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGATCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGc                                                                                 <  2:275707/149‑1 (MQ=255)
                                                                   cccAAGGCTTGCAGCTGCACGCAGCTGACCAGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGcc                                                                             <  4:13777/149‑1 (MQ=255)
                                                                    ccAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCgg                                                                               <  2:17969/146‑1 (MQ=255)
                                                                    ccAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCgg                                                                               >  1:17969/1‑146 (MQ=255)
                                                                    ccAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCg                                                                            >  3:203248/1‑149 (MQ=255)
                                                                      aaGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTc                                                                          >  1:156213/1‑149 (MQ=255)
                                                                       aGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTcc                                                                         <  6:220499/149‑1 (MQ=255)
                                                                         gCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTg                                                                       <  2:156213/149‑1 (MQ=255)
                                                                          cTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTgg                                                                      <  1:200725/149‑1 (MQ=255)
                                                                                gCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCAcg                                                                >  6:204225/1‑149 (MQ=255)
                                                                                        ccgcTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTc                                                        <  3:75192/149‑1 (MQ=255)
                                                                                              aCCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGAt                                                  <  7:85100/149‑1 (MQ=255)
                                                                                                ccGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGt                                                >  1:14495/1‑149 (MQ=255)
                                                                                                  ggTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTcc                                              <  1:115835/149‑1 (MQ=255)
                                                                                                       gACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGc                                                      >  4:219487/1‑136 (MQ=255)
                                                                                                       gACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGc                                                      <  3:219487/136‑1 (MQ=255)
                                                                                                            gTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGATTTTCGACGATGTCCACCTCGGCCa                                    >  4:169093/1‑149 (MQ=255)
                                                                                                                   ttGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCtt                             >  7:280150/1‑149 (MQ=255)
                                                                                                                     gTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGa                           >  6:233993/1‑149 (MQ=255)
                                                                                                                     gTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGC‑AGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGAt                          >  1:67905/1‑149 (MQ=255)
                                                                                                                      tCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGAt                          <  3:123311/149‑1 (MQ=255)
                                                                                                                        gAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATgc                        >  6:147101/1‑149 (MQ=255)
                                                                                                                          gCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATgcgc                      <  5:147101/149‑1 (MQ=255)
                                                                                                                           cACCCGCAGCACGGCATCTTCACCGAAGATGC‑AGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCga                    <  2:67905/149‑1 (MQ=255)
                                                                                                                              ccGCATCACGGCATCTTCACCGATGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCTGCCGTCCTGGGGCACGCGTTTTTCGcc                                                     <  5:298963/114‑1 (MQ=255)
                                                                                                                              ccGCAGCACGGCATCTACACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCACACGGCCAACTTGAAGCGGCCGTCCTGGGGCACGCCTTTTTcccc                                                     >  6:298963/1‑114 (MQ=255)
                                                                                                                                gcagcaCGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATc                <  4:203248/149‑1 (MQ=255)
                                                                                                                                 cagcaCGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATCa               >  4:121446/1‑149 (MQ=255)
                                                                                                                                   gcaCGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATCAcc             <  2:223947/149‑1 (MQ=255)
                                                                                                                                     ctagCATCTTCGGTGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATCACCTg           >  4:319847/4‑149 (MQ=255)
                                                                                                                                      tagCATCTTCGGTGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATCACCTgg          >  3:60054/3‑149 (MQ=255)
                                                                                                                                          aTCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATCACCTGGTCgg      >  6:154538/1‑149 (MQ=255)
                                                                                                                                               cACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATCACCTGGTCGGCGaa  <  3:105472/148‑1 (MQ=255)
                                                                                                                                               cACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATCACCTGGTCGGCGaa  <  3:110199/148‑1 (MQ=255)
                                                                                                                                               cACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATCACCTGGTCGGCGa   <  2:14495/147‑1 (MQ=255)
                                                                                                                                                aCCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCga                    <  4:276753/129‑1 (MQ=255)
                                                                                                                                                aCCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCga                    >  3:276753/1‑129 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GACCAGGATCATGCCGTAGGGTTCTGCCGCCAACCGGCGCAGGGCGCGCAAGGTTTCGTCGGCAAAGCCCAAGGCTTGCAGCTGCACGCCGCTGACCCGGTCGGACAAGTCCTGCTTGTCGAGCACCCGCAGCACGGCATCTTCACCGAAGATGCTAGGCATGATCGACACCCGAAAGTCGATTTGCCGGTCGCCCACGGCCACCTTGAAGCGGCCGTCCTGGGGCACGCGTTTTTCGCCGATGTCCAGCTCGGCCATGACCTTGATGCGCGAGATCACCTGGTCGGCGAA  >  NC_002947/3946984‑3947274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑