Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,067,034 A→G 7.1% L677S (TTG→TCG)  mdtC ← multidrug transporter membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,067,0340AG 7.1% 160.6 / 2.3 57L677S (TTG→TCG) mdtCmultidrug transporter membrane protein
Reads supporting (aligned to +/- strand):  ref base A (29/23);  new base G (2/2);  total (32/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

TGTCCATGTCGATGCCCAGGCGCTTGGCCTGGTCACGGTCGACCACCAGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCAGGAACAGGCGCCCGCCGGGCACTTTGGGCAGTTCCTTGCGCAGGCGC  >  NC_002947/4066888‑4067177
                                                                                                                                                  |                                                                                                                                               
tgtccatgtcGATGCCCAGGCGCTTGGCCTGGTCACGGTCGACCACCAGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAAc                                                                                                                                               >  8:292785/1‑149 (MQ=255)
     atgtcGATGCCCAGGCGCTTGGCCTGGTCACGGTCGACCACCAGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGTCGCGACCACCTTGGGAAACCATTCGCGCAAcgccgc                                                                                                                                          >  8:102463/1‑149 (MQ=255)
                cAGGCGCTTGGCCTGGTCACGGTCGCCCACCAGCCTCACCTGTTGGGTACCTGCACCATCGCGGGCGTCGATCGCGGTCAGCTCAGGCAGTGCACGCCGCGCCCCGCCCACCTTGGGCAACCAAACGCGCAACGCCGCCAGCTcgcccc                                                                                                                               >  4:292203/1‑147 (MQ=255)
                 aGGCGCTTGGCCTGGTCACGGTCGACCACCAGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCt                                                                                                                              <  6:279617/149‑1 (MQ=255)
                      gtttgCCGGGTCACGGTCTTATACCCGCGTCACCTGTTGGGTACGGGCACCTTCGCTGTTGTCGATTGCGGTCAGCTCAGGCATTGCAATTAGTTCCGCGACCACCTTTGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCa                                                                                                                         <  7:49272/145‑1 (MQ=255)
                       ttGGCCTGGTCACGGTCGACCACCAGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAg                                                                                                                        >  5:298203/1‑149 (MQ=255)
                           ccTGGTCACGGTCGACCACCAGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGta                                                                                                                    >  7:116370/1‑149 (MQ=255)
                             tGGTCACGGTCGACCACCAGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGtata                                                                                                                  >  6:181179/1‑149 (MQ=255)
                               gTCACGGTCGACCACCAGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACa                                                                                                                <  7:292785/149‑1 (MQ=255)
                                tCACGGTCGACCACCAGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCtt                                                                                                                             >  2:193192/1‑135 (MQ=255)
                                tCACGGTCGACCACCAGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCtt                                                                                                                             <  1:193192/135‑1 (MQ=255)
                                 cACGGTCGACCACCAGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCGACGCCGCCAGGTCGCCGCTTTGCCGGGTATACAgg                                                                                                              >  6:254903/1‑149 (MQ=255)
                                 cACGGTCGACCACCAGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCGACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAgg                                                                                                              >  8:201581/1‑149 (MQ=255)
                                    ggTCGACCACCAGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAAcgccg                                                                                                                                           <  4:60860/117‑1 (MQ=255)
                                    ggTCGACCACCAGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAAcgccg                                                                                                                                           >  3:60860/1‑117 (MQ=255)
                                    ggTCGACCACCAGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTAc                                                                                                           >  5:65170/1‑149 (MQ=255)
                                    ggTCGACCACCAGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTAc                                                                                                           <  7:113552/149‑1 (MQ=255)
                                          ccaccaGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTga                                                                                                        <  7:135509/146‑1 (MQ=255)
                                          ccaccaGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTga                                                                                                        >  8:135509/1‑146 (MQ=255)
                                           caccaGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGTGTATACAGGTACTGagaag                                                                                                    <  8:116370/149‑1 (MQ=255)
                                             ccaGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAagag                                                                                                  <  6:65170/149‑1 (MQ=255)
                                             ccaGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAagag                                                                                                  >  3:183057/1‑149 (MQ=255)
                                                gCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTc                                                                                               <  2:14804/149‑1 (MQ=255)
                                                  gTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCTCAACGCCGCCAGGTCACCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTg                                                                                             <  6:291098/149‑1 (MQ=255)
                                                     aCCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCCACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTgtt                                                                                           >  3:205979/1‑146 (MQ=255)
                                                          ttGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACg                                                                                       <  5:181179/147‑1 (MQ=255)
                                                            gggTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAggg                                                                                                                      >  3:66788/1‑114 (MQ=255)
                                                            gggTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAggg                                                                                                                      <  4:66788/114‑1 (MQ=255)
                                                                 ccGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGcc                                                                              >  4:75860/1‑149 (MQ=255)
                                                                  cGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAg                                                                                                               >  6:60427/1‑115 (MQ=255)
                                                                  cGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAg                                                                                                               <  5:60427/115‑1 (MQ=255)
                                                                    gCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCACCCGccccc                                                                           >  2:36122/1‑149 (MQ=255)
                                                                          tCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTg                                                                     <  3:75860/149‑1 (MQ=255)
                                                                                  gTCGATGGCGGGCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCt                                                             <  4:183057/149‑1 (MQ=255)
                                                                                        ggCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCgg                                                       >  1:240378/1‑149 (MQ=255)
                                                                                               aGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCAg                                                >  6:245635/1‑149 (MQ=255)
                                                                                                gCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCAg                                                >  2:71757/1‑148 (MQ=255)
                                                                                                  tCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCa                                                 <  2:252796/145‑1 (MQ=255)
                                                                                                  tCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCa                                                 >  1:252796/1‑145 (MQ=255)
                                                                                                    aGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCa                                                                                         >  3:55934/1‑103 (MQ=255)
                                                                                                    aGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCa                                                                                         <  4:55934/103‑1 (MQ=255)
                                                                                                      gCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCCCCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCAGGAACAgg                                         >  6:63716/1‑149 (MQ=255)
                                                                                                      gCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCAGGAACAgg                                         <  2:240378/149‑1 (MQ=255)
                                                                                                            cACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCAGGAACAGGcgcccg                                   >  5:145258/1‑149 (MQ=255)
                                                                                                             aCGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCAGGAACAGGcgcccgc                                  >  8:42546/1‑149 (MQ=255)
                                                                                                             aCGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCAGGAACAGGcgcccgc                                  >  5:286468/1‑149 (MQ=255)
                                                                                                              cGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCAGGAACAGGcgcccgcc                                 >  3:54575/1‑149 (MQ=255)
                                                                                                                 aGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCAGGAACAGGCGCCCGCCggg                              <  7:42546/149‑1 (MQ=255)
                                                                                                                     ccgcGACCACCTTGGGAAACCATTCGCGCGACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCAGGAACAGGCGCCCGCCGGGCACt                          <  7:201581/149‑1 (MQ=255)
                                                                                                                     ccgcGACCACCTTGGGAAACCATTCGCGCGACGCCGCCAGGGCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCAGGAACAGGCGCCCGCCGGGCACt                          <  5:254903/149‑1 (MQ=255)
                                                                                                                     ccgcGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCAGGAACAGGCGCCCGCCGGGCACt                          >  7:199866/1‑149 (MQ=255)
                                                                                                                       gcgACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCAGGAACAGGCGCCCGCCGGGCACttt                        <  5:245635/149‑1 (MQ=255)
                                                                                                                       gcgACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCAGGAACAGGCGCCCGCCGGGCACttt                        <  1:71757/149‑1 (MQ=255)
                                                                                                                          accaccTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCAGGAACAGGCGCCCGCCGGGCACTTTggg                     <  7:102463/149‑1 (MQ=255)
                                                                                                                                     aaaCCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCAGGAACAGGCGCCCGCCGGGCACTTTGGGCAGTTCCTTgc          >  2:185769/1‑149 (MQ=255)
                                                                                                                                          aTTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCAGGAACAGGCGCCCGCCGGGCACTTTGGGCAGTTCCTTGCGCAgg     >  2:91356/1‑149 (MQ=255)
                                                                                                                                             cgcgCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCAGGAACAGGCGCCCGCCGGGCACTTTGGGCAGTTCCTTGCGCAGgcgc  >  7:261048/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TGTCCATGTCGATGCCCAGGCGCTTGGCCTGGTCACGGTCGACCACCAGCGTCACCTGTTGGGTACCGGCACCATCGCGGGCGTCGATGGCGGTCAGCTCAGGCAGTGCACGCAGCGCCGCGACCACCTTGGGAAACCATTCGCGCAACGCCGCCAGGTCGCCGCTTTGCAGGGTATACAGGTACTGAGAAGAGGTCTGGTCACGCCCACCGCCCCCCAGTTGCAGGTCCTGGTCGGCCATCAGGAACAGGCGCCCGCCGGGCACTTTGGGCAGTTCCTTGCGCAGGCGC  >  NC_002947/4066888‑4067177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑