Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,084,759 T→A 9.1% M62K (ATG→AAG) ‡ PP_3595 → amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,084,7590TA 9.1% 121.9 / 3.5 44M62K (ATG→AAG) ‡PP_3595amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (21/19);  new base A (2/2);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

CCCGACCTGCTGGAGCGCTATGGCCCGCGCTTCATCGACGGCCTGCTGGTAACTGCCAAGCTGGTGGCGATTTCCTTCAGCCTGGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGGCTCGCTCAAAGGTTTCTGGCAGGACGTCGGCCTGTGGTGGTTCTTCCGGGATGCGTGGTTTTGCA  >  NC_002947/4084611‑4084899
                                                                                                                                                    |                                                                                                                                            
cccGACCTGCTGGAGCGCTATGGCCCGCGCTTCATCGACGGCCTGCTGGTAACTGCCAAGCTGGTGGCGATTTCCTTCAGCCTGGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCAt                                                                                                                                              >  5:193375/1‑149 (MQ=255)
     ccTGCTGGAGCGCTATGGCCCGCGCTTCATCGACGGCCTGCTGGTAACTGCCAAGCTGGTGGCGATTTCCTTCAGCCTGGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAACCATGCGCTGCAGCACCAGGCTg                                                                                                                                         >  4:226249/1‑149 (MQ=255)
         ctgGAGCGCTATGGCCCGCGCTTCATCGACGGCCTGCTGGTAACTGCCAAGCTGGTGGCGATTTCCTTCAGCCTGGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAACCATGCGCTGCAGCACCAGGCTGcg                                                                                                                                       <  3:226249/147‑2 (MQ=255)
                         cgcgCTTCATCGACGGCCTGCTGGTAACTGCCAAGCTGGTGGCGATTTCCTTCAGCCTGGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACttcttc                                                                                                                     <  6:193375/149‑1 (MQ=255)
                             cTTCATCGACGGCCTGCTGGTAACTGCCAAGCTGGTGGCGATTTCCTTCAGCCTGGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCcgcg                                                                                                                 >  6:144142/1‑149 (MQ=255)
                              ttCATCGACGGCCTGCTGGTAACTGCCAAGCTGGTGGCGATTTCATTCAGCATTGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGACTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTACTTCagcgc                                                                                                                >  7:275972/1‑148 (MQ=255)
                                  tCGACGGCCTGCTGGTAACTGCCAAGCTGGTGGCGATTTCCTTCAGCCTGGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACttcttc                                                                                                                     <  3:34834/140‑1 (MQ=255)
                                  tCGACGGCCTGCTGGTAACTGCCAAGCTGGTGGCGATTTCCTTCAGCCTGGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACttcttc                                                                                                                     >  4:34834/1‑140 (MQ=255)
                                      cGGCCTGTTGGTAACTCCCATGCTGGTGGGTATTTCCTTCAGCCTGGGGGCGGTGCTCGTTCTGCTGCTGGCCTTTGCATGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTcgccgc                                                                                                        <  1:102414/149‑1 (MQ=255)
                                          ctgctgGTAACTGCCAAGCTGGTGGCGATTTCCTTCAGCCTGGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCgctgct                                                                                                    >  3:227704/1‑149 (MQ=255)
                                             ctgGTAACTGCCAAGCTGGTGGCGATTTCCTTCAGCCTGGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGctgctg                                                                                                   <  7:46991/147‑1 (MQ=255)
                                                gTAACTGCCAAGCTGGTGGCGATTTCCTTCAGCCTGGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGgcgc                                                                                               >  4:217289/1‑148 (MQ=255)
                                                  aaCTGCCAAGCTGGTGGCGATTTCCTTCAGCCTGGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAAt                                                                                            >  1:159844/1‑149 (MQ=255)
                                                     tGCCAAGCTGGTGGCGATTTCCTTCAGCCTGGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGt                                                                                         >  5:224140/1‑149 (MQ=255)
                                                     tGCCAAGCTGGTGGCGATTTCCTTCAGCCTGGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGt                                                                                         >  6:120294/1‑149 (MQ=255)
                                                     tGCCAAGCTGGTGGCGATTTCCTTCAGCCTGGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGt                                                                                         >  1:199747/1‑149 (MQ=255)
                                                       ccAAGCTGGTGGCGATTTCCTTCAGCCTGGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTc                                                                                       >  2:139590/1‑149 (MQ=255)
                                                            cTGGTGGCGATTTCCTTCAGCCTGGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCctgct                                                                                  <  5:144142/149‑1 (MQ=255)
                                                                      tttCCTTCAGCCTGGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGc                                                                        >  2:329730/1‑149 (MQ=255)
                                                                             cAGCCTGGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGgct                                                                 >  8:132775/1‑149 (MQ=255)
                                                                                      tgctgtgctCGGTCTTGTTCTGGCCCTTGCACGCCTGGCTCGCAGCCTGGTGCTGCAGCGCATGGCGGCCGGCTACGTGTTCTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTTTATTACGGCCTTGGCTCGCTCAAAg                                                        <  4:227704/149‑1 (MQ=255)
                                                                                      tgctgtgctCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGGCTCGCTCAAAg                                                        <  6:224140/149‑1 (MQ=255)
                                                                                        ctgtgctCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTActtct                                                                                                                       <  5:103425/84‑1 (MQ=255)
                                                                                        ctgtgctCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTActtct                                                                                                                       >  6:103425/1‑84 (MQ=255)
                                                                                         tgtgctCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTcc                                                                                      >  2:262267/1‑116 (MQ=255)
                                                                                           tgctCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGGCTCGCTCAAAGGttt                                                    <  7:132775/148‑1 (MQ=255)
                                                                                            gctCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACAGCCTGGGCTCGCTCAAAg                                                        <  3:213387/143‑1 (MQ=255)
                                                                                            gctCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACAGCCTGGGCTCGCTCAAAg                                                        >  4:213387/1‑143 (MQ=255)
                                                                                             ctCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGGCTCGCTCAAAGGTTTCTg                                                 <  3:217289/149‑1 (MQ=255)
                                                                                                             cccTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGGCTCGCTCAAAGGTTTCTGGCAGGACGTCGGCCtg                                 <  2:199747/149‑1 (MQ=255)
                                                                                                               cTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGGGCTTGGGTGCGGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTTCGGCCTGGGCTCGCTCAAATGTTTCTGGGAGGACGTCGGCCtgtg                               <  1:70477/149‑1 (MQ=255)
                                                                                                                      gCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGGCTCGCTCAAAg                                                        >  2:79664/1‑117 (MQ=255)
                                                                                                                      gCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGGCTCGCTCAAAg                                                        <  1:79664/117‑1 (MQ=255)
                                                                                                                      gCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCAGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGGCTCGCTCAAAGGTTTCTGGCAGGACGTCGGCCTGTGGTGGttc                        >  7:129991/1‑149 (MQ=255)
                                                                                                                        cTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGGCTCGCTCAAAGGTTTCTGGCAGGACGTCGGCCTGTGGTGGttctt                      <  5:120294/149‑1 (MQ=255)
                                                                                                                        cTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGGCTCGCTCAAAGGTTTCTGGCAGGACGTCGGCCTGTGGTGGttctt                      >  3:309975/1‑149 (MQ=255)
                                                                                                                        cTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGACGGCTCCGTGTACTTATTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGGCTGGCTAGAAGGTTTCTGGCAGGACGTCGGCGTGTGGTGGttctt                      >  7:298978/1‑149 (MQ=255)
                                                                                                                           tctcGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGGCTCGCTCAAAGGTTTCTGGCAGGACGTCGGCCTGTGGTGGTTCTTCCg                   <  1:139590/149‑1 (MQ=255)
                                                                                                                             tcGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGGCTCGCTCAAAGGTTTCTGGCAGGACGTCGGCCtgtg                               <  2:189978/135‑1 (MQ=255)
                                                                                                                               gCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGGCTCGCTCAAAGGTTTCTGGCAGGACGTCGGCCTGTGGTGGTTCTTCCgg                  <  1:329730/146‑1 (MQ=255)
                                                                                                                                   ccTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGGCTCGCTCAAAGGTTTCTGGCAGGACGTCGGCCTGTGGTGGTTCTTCCGGGATGCGt           >  1:305301/1‑149 (MQ=255)
                                                                                                                                     atgcgctgcAGCACCAGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGGCTCGCTCaaa                                                         <  1:84754/97‑1 (MQ=255)
                                                                                                                                     atgcgctgcAGCACCAGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGGCTCGCTCaaa                                                         >  2:84754/5‑101 (MQ=255)
                                                                                                                                       gTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGGCTCGCTCAAAGGTTTCTGGCAGGACGTCGGCCTGTGGTGGTTCTTCCGGGATGCGTgg         <  8:112529/147‑1 (MQ=255)
                                                                                                                                            gcAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGGCTCGCTCAAAGGTTTCTGGCAGGACGTCGGCCTGTGGTGGTTCTTCCGGGATGCGTGGTTTTGCa  >  1:165460/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
CCCGACCTGCTGGAGCGCTATGGCCCGCGCTTCATCGACGGCCTGCTGGTAACTGCCAAGCTGGTGGCGATTTCCTTCAGCCTGGGTGCTGTGCTCGGTCTTCTGCTGGCCCTTGCACGCCTGTCTCGCAGCCTGGTGCTGCAGCGCATGGCTGCCGGCTACGTGTACTTCTTCCGCGGTTCGCCGCTGCTGGCGCAATTGTTCCTGCTGTATTACGGCCTGGGCTCGCTCAAAGGTTTCTGGCAGGACGTCGGCCTGTGGTGGTTCTTCCGGGATGCGTGGTTTTGCA  >  NC_002947/4084611‑4084899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑