Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,133,527 T→C 6.9% T148A (ACC→GCC) ‡ PP_3637 ← sulfonate ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,133,5270TC 6.9% 164.6 / 2.8 58T148A (ACC→GCC) ‡PP_3637sulfonate ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (27/27);  new base C (2/2);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01

TCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACG  >  NC_002947/4133383‑4133675
                                                                                                                                                |                                                                                                                                                    
tCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTgcgc                                                                                                                                                  >  4:69455/1‑149 (MQ=255)
  aaCACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGGAGCGGGCCAGCGCGGTGCGCTg                                                                                                                                                <  4:215118/149‑1 (MQ=255)
  aaCACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTg                                                                                                                                                >  2:154268/1‑149 (MQ=255)
      cGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGCCCAGCGCACCGCGCTGGccc                                                                                                                                            <  3:265412/149‑1 (MQ=255)
           gcgGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATgcc                                                                                                                                       >  8:35743/1‑149 (MQ=255)
               tCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCa                                                                                                                                   <  6:336479/149‑1 (MQ=255)
                   gtgCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGAc                                                                                                                                <  3:69455/148‑1 (MQ=255)
                    tgCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACa                                                                                                                               <  5:263198/148‑1 (MQ=255)
                             aTCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCgg                                                                                                                     <  7:1635/149‑1 (MQ=255)
                             aTCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCgg                                                                                                                     >  6:79859/1‑149 (MQ=255)
                                aGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGt                                                                                                                   <  4:304746/148‑1 (MQ=255)
                                aGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGAAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTa                                                                                                                  >  7:224561/1‑149 (MQ=255)
                                  ttGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACt                                                                                                                >  4:103075/1‑149 (MQ=255)
                                    gcagcagcaACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTg                                                                                                               <  6:213494/148‑1 (MQ=255)
                                        cagcaACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCAc                                                                                                          >  5:319825/1‑149 (MQ=255)
                                            aaCTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGc                                                                                                      <  5:79859/149‑1 (MQ=255)
                                                 ctgcATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTAc                                                                                                                 >  4:43710/1‑133 (MQ=255)
                                                 ctgcATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTAc                                                                                                                 <  3:43710/133‑1 (MQ=255)
                                                 ctgcATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTg                                                                                                   <  1:154268/147‑1 (MQ=255)
                                                  tgcATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGc                                                                                                >  4:219304/1‑149 (MQ=255)
                                                  tgcATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCACCGCGCTGGccc                                                                                                                                            <  6:64424/105‑1 (MQ=255)
                                                  tgcATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCACCGCGCTGGccc                                                                                                                                            >  5:64424/1‑105 (MQ=255)
                                                   gcATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGc                                                                                                >  7:161917/1‑148 (MQ=255)
                                                    cATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACgg                                                                                                        <  6:124620/139‑1 (MQ=255)
                                                    cATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACgg                                                                                                        >  5:124620/1‑139 (MQ=255)
                                                       gTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGc                                                                                           >  3:141551/1‑149 (MQ=255)
                                                         cGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGcc                                                                                          >  2:185699/1‑148 (MQ=255)
                                                          ggCGCGGCTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGCCCACGGCCGTCGGCCAGGCCca                                                                                        >  8:147059/1‑149 (MQ=255)
                                                               gggTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGcc                                                                                   <  3:103075/149‑1 (MQ=255)
                                                                     ccTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATc                                                                             >  7:28584/1‑149 (MQ=255)
                                                                       tCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCg                                                                            >  2:53100/1‑148 (MQ=255)
                                                                        cATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCa                                                                          >  1:291315/1‑149 (MQ=255)
                                                                         aTCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCAt                                                                         >  7:163312/1‑149 (MQ=255)
                                                                         aTCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCAt                                                                         >  2:110212/1‑149 (MQ=255)
                                                                            cAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGt                                                                      <  5:205672/149‑1 (MQ=255)
                                                                            cAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGt                                                                      <  4:141551/149‑1 (MQ=255)
                                                                             aaCGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCCCGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTc                                                                     >  5:222868/1‑149 (MQ=255)
                                                                                  aCCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCa                                                                          <  7:294445/139‑1 (MQ=255)
                                                                                  aCCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCa                                                                          >  8:294445/1‑139 (MQ=255)
                                                                                  aCCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGc                                                                <  8:163312/149‑1 (MQ=255)
                                                                                    cGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGt                                                              >  5:16881/1‑149 (MQ=255)
                                                                                           ttCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGc                                                       >  4:20158/1‑149 (MQ=255)
                                                                                               tCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTc                                                   <  3:219304/149‑1 (MQ=255)
                                                                                                 cAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCg                                                  <  1:53100/148‑1 (MQ=255)
                                                                                                 cAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCg                                                  <  5:220586/148‑1 (MQ=255)
                                                                                                  aGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCgg                                                 >  1:98023/1‑148 (MQ=255)
                                                                                                                 ggCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGTCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTggg                                 <  1:110212/149‑1 (MQ=255)
                                                                                                                 ggCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTggg                                 <  8:161917/149‑1 (MQ=255)
                                                                                                                             aGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGaa                     <  2:291315/149‑1 (MQ=255)
                                                                                                                               gCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTgggg                                >  1:75672/1‑135 (MQ=255)
                                                                                                                               gCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGc                                <  2:75672/136‑1 (MQ=255)
                                                                                                                                  gCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGa                      >  4:253319/1‑143 (MQ=255)
                                                                                                                                  gCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGa                      <  3:253319/143‑1 (MQ=255)
                                                                                                                                  gCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCa                <  8:224561/149‑1 (MQ=255)
                                                                                                                                    gggCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAgg              <  6:16881/149‑1 (MQ=255)
                                                                                                                                    gggCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAgg              >  5:229343/1‑149 (MQ=255)
                                                                                                                                     gtccAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCTAAGTCCAGGc             <  6:319825/147‑1 (MQ=255)
                                                                                                                                       ccAGCGCACCGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGcc            >  1:5354/1‑148 (MQ=255)
                                                                                                                                                tGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGACGAAGTCCAGGCCGAAGGCGACg  <  7:35743/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCACGGGCGTGGGCCAGGCCCACAGCCTCAATCGCATGGTCGATGCGTGCACGGCGTTCGGCATCCGCCAGCCTGGGCTGACGGGCGAAGTCCAGGCCGAAGGCGACG  >  NC_002947/4133383‑4133675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑