Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,138,575 A→C 6.6% D25A (GAC→GCC)  PP_3643 → oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,138,5750AC 6.6% 176.8 / 2.6 57D25A (GAC→GCC) PP_3643oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (25/28);  new base C (2/2);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ATTACGGTATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTG  >  NC_002947/4138431‑4138720
                                                                                                                                                 |                                                                                                                                                 
aTTACGGTATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGgacgac                                                                                                                                               >  3:312117/1‑149 (MQ=255)
 ttACGGTATACCATCATACACATCAAAGATAAAATCCGCGGACCACCGCAGCCCTTGAGAGGTACTCCCGATGATCGACGCTACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGgacgac                                                                                                                                               <  4:312117/148‑1 (MQ=255)
 ttACGGTATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGgacgac                                                                                                                                               <  5:285467/148‑1 (MQ=255)
    cGGTATACCATCAGACACATCAACGATAAAAACCGCTGACCACCGAAGCCGTTCAGTTGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCATGacgacgacg                                                                                                                                           <  8:94591/149‑1 (MQ=255)
    cGGTATACAATCACACACAGCAACGATAAAAACCGCGGACCCCCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAACAACGTTCTGGGCTCCTTCCCGTACGGCGTTACCGTCATCA‑CCACCCAGgacgacgac                                                                                                                                            <  1:211113/148‑1 (MQ=255)
                aGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGtcgtcg                                                                                                                               <  7:246927/149‑1 (MQ=255)
                  acacATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGc                                                                                                                             >  7:307277/1‑149 (MQ=255)
                      aTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGa                                                                                                                         >  6:77457/1‑149 (MQ=255)
                       tCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGCCGACGACCGAGCCGTCGTCGTCCTggg                                                                                                                        >  1:69986/1‑147 (MQ=255)
                       tCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGCCGACGACCGAGCCGTCGTCGTCCTggg                                                                                                                        <  2:69986/149‑3 (MQ=255)
                              tAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAgc                                                                                                                 <  1:297757/149‑1 (MQ=255)
                                  aaCCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCt                                                                                                             >  3:151071/1‑149 (MQ=255)
                                   aCCGCGGCCCACCGCAGCCCTTCAGCGGTACTCCCGCTGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCtt                                                                                                            <  1:325445/149‑1 (MQ=255)
                                   aCCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCtt                                                                                                            <  5:176933/149‑1 (MQ=255)
                                     cgcgGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTtct                                                                                                          >  5:325531/1‑149 (MQ=255)
                                          accaccGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCtcctcc                                                                                                     >  4:276369/1‑149 (MQ=255)
                                          accaccGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCtcctcc                                                                                                     <  6:325531/149‑1 (MQ=255)
                                            caccGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCt                                                                                                   >  1:325978/1‑149 (MQ=255)
                                                 cAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTACCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTTAACGCCAGCGCCTTCTCCTCCCTGTCGa                                                                                              >  3:261750/1‑149 (MQ=255)
                                                       ttCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGAcc                                                                                        >  5:122344/1‑149 (MQ=255)
                                                         cAGAGGTACTCCCGATGATCGACGCAACTGTCTACCAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGAccc                                                                                       <  8:307277/148‑1 (MQ=255)
                                                            agGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACccgccg                                                                                   >  3:271102/1‑149 (MQ=255)
                                                              gTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCt                                                                                 <  6:122344/149‑1 (MQ=255)
                                                               tACTCCCGATGATCGACTCAACTGGCTACAATAACGTGGTGGGCTCCTTCCCGTCCGGCGTAACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTg                                                                                <  2:325978/149‑1 (MQ=255)
                                                                    ccGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGc                                                                                                                             <  5:52210/99‑1 (MQ=255)
                                                                    ccGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGc                                                                                                                             >  6:52210/1‑99 (MQ=255)
                                                                    ccGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCt                                                                           >  7:250972/1‑149 (MQ=255)
                                                                       atgatCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGtt                                                                        <  3:276369/149‑1 (MQ=255)
                                                                             gACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGccc                                                                  >  4:113998/1‑149 (MQ=255)
                                                                                  aaCTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCaa                                                               >  1:86597/1‑148 (MQ=255)
                                                                                       tCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTacac                                                         >  3:199102/1‑148 (MQ=255)
                                                                                         tACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCa                                                                                                                   <  7:232832/88‑1 (MQ=255)
                                                                                         tACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCa                                                                                                                   >  8:232832/1‑88 (MQ=255)
                                                                                         tACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTc                                                      >  6:287057/1‑149 (MQ=255)
                                                                                          aCAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTcc                                                     >  5:306088/1‑149 (MQ=255)
                                                                                          aCAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTcc                                                     >  6:292583/1‑149 (MQ=255)
                                                                                           cAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCt                                                       <  4:151071/146‑1 (MQ=255)
                                                                                           cAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTcc                                                     <  4:271102/148‑1 (MQ=255)
                                                                                            aaGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTg                                                                          >  4:157435/1‑126 (MQ=255)
                                                                                            aaGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTg                                                                          <  3:157435/126‑1 (MQ=255)
                                                                                             aGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGAc                                                  <  6:306088/149‑1 (MQ=255)
                                                                                              gAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACt                                                 >  3:55508/1‑149 (MQ=255)
                                                                                                   ttCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTAc                                            <  3:113998/149‑1 (MQ=255)
                                                                                                   ttCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTAc                                            <  5:1467/149‑1 (MQ=255)
                                                                                                         gCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTa                                      <  2:86597/149‑1 (MQ=255)
                                                                                                          cTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTAc                                     <  8:250972/149‑1 (MQ=255)
                                                                                                          cTCCTGCCCGTCCGCCTGTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCTGTAc                                     <  4:261750/149‑1 (MQ=37)
                                                                                                             cTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTccc                                                                                                    <  2:189122/83‑1 (MQ=255)
                                                                                                             cTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTccc                                                                                                    >  1:189122/1‑83 (MQ=255)
                                                                                                             cTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGa                                  <  5:77457/149‑1 (MQ=255)
                                                                                                               tCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGAt                                 >  4:84697/1‑148 (MQ=255)
                                                                                                                 ccGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCaa                              <  4:55508/149‑1 (MQ=255)
                                                                                                                             aCCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCt                   <  3:262904/148‑1 (MQ=255)
                                                                                                                              ccGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCttt                 >  6:188688/1‑149 (MQ=255)
                                                                                                                                        ccaccCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGaa                        <  7:73266/133‑1 (MQ=255)
                                                                                                                                        ccaccCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGaa                        >  8:73266/1‑133 (MQ=255)
                                                                                                                                           cccAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACctg     <  5:188688/149‑1 (MQ=255)
                                                                                                                                           cccAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCACTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACctg     <  3:321490/149‑1 (MQ=255)
                                                                                                                                            tcAGGCCGACGACCGAGCCGTCGTCGTCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTa                                      <  2:208595/114‑1 (MQ=25)
                                                                                                                                            tcAGGCCGACGACCGAGCCGTCGTCGTCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTa                                      >  1:208595/2‑115 (MQ=25)
                                                                                                                                             cAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACctgct   <  5:287057/149‑1 (MQ=255)
                                                                                                                                              aGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACctgctg  >  7:295576/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
ATTACGGTATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCA‑CCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTG  >  NC_002947/4138431‑4138720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑