Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,149,781 C→G 7.4% P78R (CCG→CGG)  PP_3654 → leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,149,7810CG 7.4% 193.5 / 2.3 63P78R (CCG→CGG) PP_3654leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  ref base C (27/31);  new base G (2/3);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

GCCTGTCACCCTGCCATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGTTTCAGAAGCTGTATGACGAGCACCTGACCA  >  NC_002947/4149637‑4149924
                                                                                                                                                |                                                                                                                                               
gCCTGTCACCCTGCCATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTg                                                                                                                                             <  8:199594/149‑1 (MQ=255)
 ccTGTCACCCTGCCATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTgg                                                                                                                                            >  2:113112/1‑149 (MQ=255)
  cTGTCACCCTGCCATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGc                                                                                                                                           <  2:1451/149‑1 (MQ=255)
     tCACCCTGCCATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCg                                                                                                                                          <  1:113112/147‑1 (MQ=255)
     tCACCCTGCCATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGa                                                                                                                                        <  5:225602/149‑1 (MQ=255)
       aCCCTGCCATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCACCGGCTGGCgggt                                                                                                                                       <  1:152842/148‑3 (MQ=255)
         ccTGCCATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACttt                                                                                                                                    >  1:141117/1‑149 (MQ=255)
           tGCCATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTg                                                                                                                                   >  3:230721/1‑148 (MQ=255)
             ccATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGaaa                                                                                                                                <  8:282332/149‑1 (MQ=255)
                      cTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTgg                                                                                                                       <  2:141117/149‑1 (MQ=255)
                         gAAGGCACTGGAAGAGTCCGGATCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGgc                                                                                                                      <  8:217834/147‑1 (MQ=255)
                              cACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATc                                                                                                               >  5:243914/1‑149 (MQ=255)
                              cACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATc                                                                                                               <  1:328810/149‑1 (MQ=255)
                               aCTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCATTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATct                                                                                                              >  1:119334/1‑148 (MQ=255)
                                   gAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCg                                                                                                            <  8:294943/147‑1 (MQ=255)
                                   gAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCg                                                                                                            >  2:249318/1‑147 (MQ=255)
                                   gAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCa                                                                                                          >  7:294943/1‑149 (MQ=255)
                                           cGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTc                                                                                                   >  2:97016/1‑148 (MQ=255)
                                                   ttCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAg                                                                                          >  1:56928/1‑149 (MQ=255)
                                                   ttCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAg                                                                                          >  3:145642/1‑149 (MQ=255)
                                                          cTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGaa                                                                                                >  7:308366/1‑136 (MQ=255)
                                                          cTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGaa                                                                                                <  8:308366/136‑1 (MQ=255)
                                                            aTCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTc                                                                                 >  8:243012/1‑149 (MQ=255)
                                                             tCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTc                                                                                 <  1:97016/148‑1 (MQ=255)
                                                                      gCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCgg                                                                       <  5:88896/149‑1 (MQ=255)
                                                                                  cgcCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCGGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCtgctgc                                                           <  6:243914/149‑1 (MQ=255)
                                                                                     ccTTGGTCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCAtg                                                        >  7:35126/1‑149 (MQ=255)
                                                                                     ccTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCAtg                                                        >  6:39999/1‑149 (MQ=255)
                                                                                         gggCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCAtgtg                                                      <  4:133382/147‑1 (MQ=255)
                                                                                         gggCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTgg                                                     >  7:71211/1‑148 (MQ=255)
                                                                                               aaCTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGcc                                                >  1:166067/1‑147 (MQ=255)
                                                                                               aaCTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCaa                                              <  1:249318/149‑1 (MQ=255)
                                                                                               aaCTTTGCCGAGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGcc                                                <  5:324674/147‑1 (MQ=255)
                                                                                                      ccGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGAt                                                                     >  2:143784/1‑119 (MQ=255)
                                                                                                      ccGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGAt                                                                     <  1:143784/119‑1 (MQ=255)
                                                                                                      ccGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCt                                        <  7:243012/148‑1 (MQ=255)
                                                                                                       cgcgCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTa                                                   >  6:13876/1‑136 (MQ=255)
                                                                                                       cgcgCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTa                                                   <  5:13876/136‑1 (MQ=255)
                                                                                                       cgcgCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGc                                      <  4:230721/149‑1 (MQ=255)
                                                                                                       cgcgCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGc                                      >  7:1881/1‑149 (MQ=255)
                                                                                                        gcgcTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGcc                                     >  6:68061/1‑149 (MQ=255)
                                                                                                              gTATTCGTGTCGCTACGCGAGTTAACCCGCCAGCCGGTGTCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCAGGATTACCTGCTGCATGTGGTAGCCAAGTACCTGCCGGCGt                                <  1:241002/148‑1 (MQ=255)
                                                                                                                aTTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGttt                              >  2:40277/1‑148 (MQ=255)
                                                                                                                    gTGACGCTACGCGAGGTCACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGTTTCAg                           >  4:211796/1‑147 (MQ=255)
                                                                                                                    gTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGTTTCAg                           >  8:229652/1‑147 (MQ=255)
                                                                                                                    gTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGTTTCAg                           >  1:232366/1‑147 (MQ=255)
                                                                                                                     tGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGTTTCAGAAg                        <  5:39999/149‑1 (MQ=255)
                                                                                                                         gCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGTTTCAGAAGCTGt                    <  5:68061/149‑1 (MQ=255)
                                                                                                                             cgcgAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGTTTCAGAAGCTGTAt                  <  2:166067/147‑1 (MQ=255)
                                                                                                                             cgcgAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGTTTCAGAAGCTGTATg                 <  7:229652/148‑1 (MQ=255)
                                                                                                                                 aGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGTTTCAGAAGCTGTATGACGAg            <  8:35126/149‑1 (MQ=255)
                                                                                                                                     agtccGCCACCGGCTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGTTTCAGAAGCTGTAt                  <  6:187874/136‑1 (MQ=255)
                                                                                                                                     agtccGCCACCGGCTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGTTTCAGAAGCTGTAt                  >  5:187874/4‑139 (MQ=255)
                                                                                                                                     aaCCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGTTTCAGAAGCTGTATGACGAGCAcc        >  4:88319/1‑149 (MQ=255)
                                                                                                                                     aaCCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGAACCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGTTTCAGAAGCTGTATGACGAGCAcc        >  5:54485/1‑149 (MQ=255)
                                                                                                                                       tccGCCACCGGCTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGt                                >  1:195875/2‑123 (MQ=255)
                                                                                                                                       tccGCCACCGGCTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGt                                <  2:195875/122‑1 (MQ=255)
                                                                                                                                        ccGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGTTTCAGAAGCTGTATGACGAGCACCTGa     >  3:94222/1‑149 (MQ=255)
                                                                                                                                        ccGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGTTTCAGAAGCTGTATGACGAGCACCTGa     <  3:88319/149‑1 (MQ=255)
                                                                                                                                         cGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGTTTCAGAAGCTGTATGACGAGCACCTGAc    <  3:211796/149‑1 (MQ=255)
                                                                                                                                           ccAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTTGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGTTTCAGAAGCTGTATGACGAGCACCTGACCa  <  5:337003/149‑1 (MQ=255)
                                                                                                                                           ccAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGTTTCAGAAGCTGTATGACGAGCACCTGACCa  <  2:119334/149‑1 (MQ=255)
                                                                                                                                           ccAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGAACCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGTTTCAGAAGCTGTATGACGAGCACCTGACCa  <  6:54485/149‑1 (MQ=255)
                                                                                                                                               ccGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGttt                              >  7:61149/1‑117 (MQ=255)
                                                                                                                                               ccGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGttt                              <  8:61149/117‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
GCCTGTCACCCTGCCATCGGCGCTTGAAGGCACTGGAAGAGTCCGGAGCCATTCTGGGCTATCACGCACGGCTGGCACCGAGCGCCCTTGGGCTGAACTTTGCCGCGCTGGTATTCGTGACGCTACGCGAGGTAACCCGCCAGCCGGTGGCGGACTTTGAAACGGCACTGGCGCAGATCCCGCAGATTGTCGAAGCGCAGCGGCTGTTCGGCGACCCGGATTACCTGCTGCATGTGGTAGCCAAGGACCTGCCGGCGTTTCAGAAGCTGTATGACGAGCACCTGACCA  >  NC_002947/4149637‑4149924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑