Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,163,505 C→A 8.0% intergenic (‑44/‑138) pssA ← / → PP_3665 CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase/AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,163,5050CA 8.0% 153.6 / 3.2 50intergenic (‑44/‑138)pssA/PP_3665CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase/AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (21/25);  new base A (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

CAGGCAGGCGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAGGAACAGCGCAATGG  >  NC_002947/4163363‑4163646
                                                                                                                                              |                                                                                                                                             
caggcaggCGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCggg                                                                                                                                         <  2:42523/149‑1 (MQ=255)
caggcaggCGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCggg                                                                                                                                         <  4:227012/149‑1 (MQ=255)
      ggcGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCAtt                                                                                                                                   >  7:183901/1‑149 (MQ=255)
        cgcgGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTcc                                                                                                                                 >  1:36598/1‑149 (MQ=255)
             tAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTc                                                                                                                                  >  6:261313/1‑143 (MQ=255)
             tAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTc                                                                                                                                  <  5:261313/143‑1 (MQ=255)
                 cccTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTg                                                                                                                        <  6:309578/149‑1 (MQ=255)
                 cccTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTg                                                                                                                        >  2:201637/1‑149 (MQ=255)
                   cTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCCGCCGGCAGGCAGGGccga                                                                                                                                    >  6:237082/1‑133 (MQ=255)
                   cTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCCGCCGGCAGGCAGGGccga                                                                                                                                    <  5:237082/135‑3 (MQ=255)
                      gCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATcc                                                                                                                   >  8:88072/1‑149 (MQ=255)
                         tCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCggg                                                                                                                <  2:126207/149‑1 (MQ=255)
                         tCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGGTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCggg                                                                                                                <  7:126285/149‑1 (MQ=255)
                           gggCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCgggg                                                                                                               <  4:80149/148‑1 (MQ=255)
                           gggCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGgt                                                                                                              <  7:88072/149‑1 (MQ=255)
                              cagcaCCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTa                                                                                                           >  8:125083/1‑149 (MQ=255)
                                   ccTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATc                                                                                                      <  1:201637/149‑1 (MQ=255)
                                   ccTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATc                                                                                                      <  1:237163/149‑1 (MQ=255)
                                   ccTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATc                                                                                                      <  7:125083/149‑1 (MQ=255)
                                    cTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATc                                                                                                      >  5:89359/1‑148 (MQ=255)
                                      ggATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCggg                                                                                                                                         <  3:92051/111‑1 (MQ=255)
                                      ggATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCggg                                                                                                                                         >  4:92051/1‑111 (MQ=255)
                                             ttCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCaa                                                                                            >  7:241757/1‑149 (MQ=255)
                                                     ccATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGTGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCAt                                                                                    <  2:36598/149‑1 (MQ=255)
                                                          gCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGc                                                                                                                          >  8:78713/1‑106 (MQ=255)
                                                                 cTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAAc                                                                        >  5:243342/1‑149 (MQ=255)
                                                                  tGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATaaca                                                                                >  6:319183/1‑140 (MQ=255)
                                                                  tGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATaaca                                                                                <  5:319183/140‑1 (MQ=255)
                                                                    gCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCAc                                                                     >  7:152910/1‑149 (MQ=255)
                                                                      aaGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCAcgc                                                                   <  6:89359/149‑1 (MQ=255)
                                                                                     gTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTtct                                                    <  8:152910/149‑1 (MQ=255)
                                                                                      tttCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTtctc                                                   <  6:243342/149‑1 (MQ=255)
                                                                                      tttCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACCCGAGGCTCTGCATtct                                                    >  2:97901/1‑148 (MQ=255)
                                                                                       ttCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAacaaca                                                                             <  7:245017/122‑1 (MQ=255)
                                                                                       ttCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAacaaca                                                                             >  8:245017/1‑122 (MQ=255)
                                                                                        tCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCAc                                                 >  4:88423/1‑149 (MQ=255)
                                                                                          ggggTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTg                                               <  4:44625/149‑1 (MQ=255)
                                                                                             gTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGccc                                            >  3:8196/1‑149 (MQ=255)
                                                                                                 aCTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGa                                        <  3:88423/149‑1 (MQ=255)
                                                                                                    gACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGccc                                            >  7:184706/1‑142 (MQ=255)
                                                                                                    gACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGccc                                            <  8:184706/142‑1 (MQ=255)
                                                                                                                     tgCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCtt                      <  4:8196/147‑1 (MQ=255)
                                                                                                                     tgCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGc                    <  8:241757/149‑1 (MQ=255)
                                                                                                                        aaTCACTGTCGGCCCTGCCTGCCGGCGGGACCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCt                                          >  4:85667/1‑124 (MQ=255)
                                                                                                                        aaTCACTGTCGGCCCTGCCTGCCGGCGGGACCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCt                                          <  3:85667/124‑1 (MQ=255)
                                                                                                                         aTCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAg                >  4:141408/1‑149 (MQ=255)
                                                                                                                                   gCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAg                >  3:126031/1‑139 (MQ=255)
                                                                                                                                   gCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAg                <  4:126031/139‑1 (MQ=255)
                                                                                                                                    ccccGCCGGCAGGCAGTGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGc                        >  6:274499/1‑130 (MQ=255)
                                                                                                                                    ccccGCCGGCAGGCAGTGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGc                        <  5:274499/130‑1 (MQ=255)
                                                                                                                                         cctgccGGCGGGGCCATTCCTGGCCGCTTCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAGGAACAGCGCAATgg  <  2:289956/147‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                             
CAGGCAGGCGCGGTAAGCCCTGGCATCGGGCAGCACCTGGATCGCTTCGGGGGCCATGGCGTAGCCTGGCAAGCCCGCCAGCATGGTTTCGGGGTTCACTGACGCTCACCTTCTCTGTGCAATCACTGTCGGCCCTGCCTGCCGGCGGGGCCATTCCTGGCCGCTGCATCCGGGGTGTATGATCGACCCGGCAACGACCCATAACAACAAGAACCACGCGAGGCTCTGCTTTCTCACTGCCCCTGACCAACCAGGCCTCAGCTTGCACAGGAACAGCGCAATGG  >  NC_002947/4163363‑4163646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑