Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,201,710 A→T 6.1% V2022V (GTT→GTA)  PP_3691 ← DNA helicase‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,201,7100AT 6.1% 199.6 / 2.7 65V2022V (GTT→GTA) PP_3691DNA helicase‑related protein
Reads supporting (aligned to +/- strand):  ref base A (28/33);  new base T (2/2);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

ATCTGCTCCAAAGTGGTTGTATAGCTCGCCTCAAAAAATCGGTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATCCACTACCTCAATGACATCAGCCATCGCTGCATCGACCTTCAGGCGCTCCGCTTCATGC  >  NC_002947/4201564‑4201857
                                                                                                                                                  |                                                                                                                                                   
atcTGCTCCAAAGTGGTTGTATAGCTCGCCTCAAAAAATCGGTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTaa                                                                                                                                                    <  8:160624/148‑1 (MQ=255)
    gttCCAAAGTGGTTGTATAGCTCGCCTCAAAAAATCGGTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGct                                                                                                                                               >  2:235942/3‑149 (MQ=255)
     cTCCAAAGTGGTTGTATAGCTCGCCTCAAAAAATCGGTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGctc                                                                                                                                              >  5:58214/1‑149 (MQ=255)
        cAAAGTGGTTGTATAGCTCGCCTCAAAAAATCGGTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGctct                                                                                                                                             <  5:145050/147‑1 (MQ=255)
        cAAAGTGGTTGTATAGCTCGCCTCAAAAAATCGGTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTg                                                                                                                                            <  6:248450/148‑1 (MQ=255)
          aaGTGGTTGTATAGCTCGCCTCAAAAAATCGGTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCg                                                                                                                                         <  1:316904/149‑1 (MQ=255)
             tGGTTGTATAGCTCGCCTCAAAAAATCGGTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTAc                                                                                                                                      <  3:231246/149‑1 (MQ=255)
              ggTTGTATAGCTCGCCTCAAAAAATCGGTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACt                                                                                                                                     >  4:47324/1‑149 (MQ=255)
                  gTATAGCTCGCCTCAAAAAATCGGTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCaa                                                                                                                                 >  5:279745/1‑149 (MQ=255)
                  gTATAGCTCGCCTCAAAAAATCGGTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCa                                                                                                                                  <  3:47324/148‑1 (MQ=255)
                   tctaGCTCGCCTCAAAAACTCGGTCTGGATCAACTGCTTCGACAGCCGCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCaa                                                                                                                                 <  8:233925/146‑1 (MQ=255)
                    ataGCTCGCCTCAAAAAATCGGTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACTACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACt                                                                                                                               >  1:79248/1‑149 (MQ=255)
                      aGCTCGCCTCAAAAAATCGGTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACt                                                                                                                               >  7:99965/1‑147 (MQ=255)
                              tCAAAAAATCGGTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTTACGGctcaag                                                                                                                                           >  2:97527/1‑124 (MQ=255)
                              tCAAAAAATCGGTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTTACGGctcaag                                                                                                                                           <  1:97527/127‑4 (MQ=255)
                                aaaaaaTCGGTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCAc                                                                                                                   <  8:37101/149‑1 (MQ=255)
                                  aaaaTCGGTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTg                                                                                                                                            <  4:56/122‑1 (MQ=255)
                                  aaaaTCGGTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTg                                                                                                                                            >  3:56/1‑122 (MQ=255)
                                   aaaTCGGTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCAc                                                                                                                   >  5:329162/1‑146 (MQ=255)
                                         gTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGAt                                                                                                          >  5:288276/1‑149 (MQ=255)
                                              gATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCAttt                                                                                                     <  2:79248/149‑1 (MQ=255)
                                              gATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCAttt                                                                                                     <  6:288276/149‑1 (MQ=255)
                                                  aaCTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTa                                                                                                 <  6:58214/149‑1 (MQ=255)
                                                   aCTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTAc                                                                                                <  7:177997/149‑1 (MQ=255)
                                                    cTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACg                                                                                               >  6:211371/1‑149 (MQ=255)
                                                      gCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTTACGGctcaag                                                                                                                                           <  3:79189/103‑4 (MQ=255)
                                                      gCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTTACGGctcaag                                                                                                                                           >  4:79189/1‑100 (MQ=255)
                                                        ttCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAgg                                                                                                                           >  2:20727/1‑117 (MQ=255)
                                                         tCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAAt                                                                                          >  5:136999/1‑149 (MQ=255)
                                                             cAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATtgtg                                                                                      <  6:329162/149‑1 (MQ=255)
                                                              aGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTgg                                                                                     <  1:235942/149‑1 (MQ=255)
                                                              aGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTgg                                                                                     <  7:162158/149‑1 (MQ=255)
                                                              aGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTgg                                                                                     <  8:99965/149‑1 (MQ=255)
                                                              aGCCACGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACATCCACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTgg                                                                                     >  6:219586/1‑149 (MQ=255)
                                                                   cGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGaa                                                                                >  8:253161/1‑149 (MQ=255)
                                                                    ggTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCAc                                                                                                                   <  2:277878/113‑1 (MQ=255)
                                                                    ggTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAg                                                                               <  3:162946/149‑1 (MQ=255)
                                                                          gTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTacaa                                                                         <  2:322682/149‑1 (MQ=255)
                                                                           tCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTacaac                                                                        <  6:279745/149‑1 (MQ=255)
                                                                           tCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTacaac                                                                        <  6:136999/149‑1 (MQ=255)
                                                                               cttcttTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTacaaca                                                                       <  3:169120/146‑1 (MQ=255)
                                                                               cttcttTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGc                                                                     <  7:253161/148‑1 (MQ=255)
                                                                                      gTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGAt                                                             >  4:164482/1‑149 (MQ=255)
                                                                                       tAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATc                                                            >  3:118537/1‑149 (MQ=255)
                                                                                        aaaCCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATc                                                            <  4:122766/148‑1 (MQ=255)
                                                                                         aaCCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATCCa                                                          >  1:265831/1‑149 (MQ=255)
                                                                                          aCCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATCCAc                                                         >  6:249960/1‑149 (MQ=255)
                                                                                              aGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATCCACTAcc                                                     >  7:242267/1‑149 (MQ=255)
                                                                                                     cACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATCCACTACCTCAAt                                                <  4:63283/147‑1 (MQ=255)
                                                                                                             cATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATCCACTACCTCAATGACATCAGcc                                      <  5:211371/149‑1 (MQ=255)
                                                                                                                aaGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGCTCCACTACCTCAATGCCATCAGCCATc                                   >  4:98992/1‑149 (MQ=255)
                                                                                                                   gAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTg                                                               <  6:322671/118‑1 (MQ=255)
                                                                                                                   gAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTg                                                               >  5:322671/1‑118 (MQ=255)
                                                                                                                   gAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATCCACTACCTCAAt                                                >  6:165118/1‑133 (MQ=255)
                                                                                                                   gAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATCCACTACCTCAAt                                                <  5:165118/133‑1 (MQ=255)
                                                                                                                          cACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATCCACTACCTCAATGACATCAGCCATCGCTGCATCGa                         >  7:258515/1‑149 (MQ=255)
                                                                                                                          cACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGATGATCCACTACCTCAATGACATCAGCCATCGCTGCATCGa                         >  1:272305/1‑149 (MQ=255)
                                                                                                                            cTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATCCACTACCTCAATGACATCAGCCATCGCTGCATCGAcc                       <  2:265831/149‑1 (MQ=255)
                                                                                                                                 tCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATCCACTACCTCAAt                                                <  2:10906/119‑1 (MQ=255)
                                                                                                                                 tCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATCCACTACCTCAAt                                                >  1:10906/1‑119 (MQ=255)
                                                                                                                                 tCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATCCACTACCTCAATGACATCAGCCATCGCTGCATCGACCTTCAg                  >  7:233649/1‑149 (MQ=255)
                                                                                                                                   aaTGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATCCACTACCTCAATGACATCAGCCATCGCTGCATCGACCTTCAg                  <  4:118537/147‑1 (MQ=255)
                                                                                                                                        cTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATCCACTACCTCAATGACATCAGCCATCGCTGCATCGACCTTc                    >  6:95110/1‑140 (MQ=255)
                                                                                                                                        cTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATCCACTACCTCAATGACATCAGCCATCGCTGCATCGACCTTc                    <  5:95110/140‑1 (MQ=255)
                                                                                                                                             gCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATCCACTACCTCAATGACATCAGCCATCGCTGCATCGACCTTCAGGCGCTCCGCTTc      <  3:164482/149‑1 (MQ=255)
                                                                                                                                                 tAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATCCACTACCTCAATGACATCAGCCATCGCTGCATCGACCTTCAGGCGCTCCGCTTCATgc  >  5:74231/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ATCTGCTCCAAAGTGGTTGTATAGCTCGCCTCAAAAAATCGGTCTGGATCAACTGCTTCGACAGCCTCGGTAGGGTCTGCTTCTTTGTAAACCAAGTGCTCCACCGGAACATAAGGAATGACCACTTCCTCAATGGCTTGAGCCGTAACGGCTCTGGCGTACTTCAACTCAGGCAATGCACCAGACGGATCATTTGCTACGGCAATTGTGGGAGAAGCTACAACAGCAAGCTGATCCACTACCTCAATGACATCAGCCATCGCTGCATCGACCTTCAGGCGCTCCGCTTCATGC  >  NC_002947/4201564‑4201857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑