Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,248,898 C→T 7.2% A377T (GCG→ACG)  PP_3724 ← acyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,248,8980CT 7.2% 155.9 / 2.1 55A377T (GCG→ACG) PP_3724acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (25/26);  new base T (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

AGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGTTGACCAGGTTGGCATAGCCCGGCAACACCTCGGCCAGTGCCGCCACCTGCCCTTG  >  NC_002947/4248758‑4249042
                                                                                                                                            |                                                                                                                                                
aggcaACAGTTCCGGGAAAGCCGAGGTGACGAACCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCcg                                                                                                                                          >  7:93157/1‑149 (MQ=255)
    aaCAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCgcggc                                                                                                                                      >  3:181775/1‑149 (MQ=255)
       aGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATcgcc                                                                                                                                                 >  1:70479/1‑135 (MQ=255)
       aGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATcgcc                                                                                                                                                 <  2:70479/135‑1 (MQ=255)
         ttCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGcgccgc                                                                                                                                 >  4:195316/1‑149 (MQ=255)
            cGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCTGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCa                                                                                                                              <  5:70072/149‑1 (MQ=255)
               gAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAggg                                                                                                                           >  4:42263/1‑149 (MQ=255)
                   gCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCcgcg                                                                                                                                        <  8:132576/132‑1 (MQ=255)
                   gCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCcgcg                                                                                                                                        >  7:132576/1‑132 (MQ=255)
                     cGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGtgttgt                                                                                                                     <  3:174965/149‑1 (MQ=255)
                       aGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCg                                                                                                                   >  4:258397/1‑149 (MQ=255)
                          tGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGc                                                                                                                <  8:278630/149‑1 (MQ=255)
                            aCGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCa                                                                                                               <  2:96843/148‑1 (MQ=255)
                              gAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGc                                                                                                            >  4:257031/1‑149 (MQ=255)
                                   cGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCg                                                                                                           <  6:311938/145‑1 (MQ=255)
                                   cGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCg                                                                                                           >  5:311938/1‑145 (MQ=255)
                                          gCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGc                                                                                                       <  3:165766/142‑1 (MQ=255)
                                            cGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGTCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGggcggc                                                                                               >  7:149587/1‑148 (MQ=255)
                                            cGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCt                                                                                              >  7:307036/1‑149 (MQ=255)
                                            cGTGGCGTTCAACGGAATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTTCGTTCGCCGGTAAACGCCTCCGGGTAGTCCAGCACCAGCATCGTCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGAGGCt                                                                                              >  7:145324/1‑149 (MQ=255)
                                                 cGTTCAACGGCATCGGAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGTCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATc                                                                                         <  8:149587/149‑1 (MQ=255)
                                                 cGTTCAAAGGCATCTCATTACAGACCATGCAGCATGTCGCATTCCTTTCGTTCGCCGGTAACCGCCGCCGGGTAGTCCAGCACCAGCATAGTCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGTCTTCATc                                                                                         <  8:145324/149‑1 (MQ=255)
                                                   ttCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGc                                                                                       >  1:76293/1‑149 (MQ=255)
                                                     cAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGccc                                                                                     >  7:29865/1‑149 (MQ=255)
                                                       aCGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGTCTTCATCGCCCCa                                                                                   <  8:93157/149‑1 (MQ=255)
                                                        cGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGccc                                                                                     >  4:112743/1‑146 (MQ=255)
                                                                cAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAgt                                                                          >  5:30833/1‑149 (MQ=255)
                                                                 aGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAgtg                                                                         >  4:307660/1‑149 (MQ=255)
                                                                   tACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAgtggt                                                                       >  7:154909/1‑149 (MQ=255)
                                                                    aCAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAgtggt                                                                       <  3:257031/148‑1 (MQ=255)
                                                                     cAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAgtggtg                                                                      <  4:181775/148‑1 (MQ=255)
                                                                      aGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAgtggtg                                                                      >  3:8510/1‑147 (MQ=255)
                                                                      aGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGaa                                                                    <  8:307036/149‑1 (MQ=255)
                                                                        ccccAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGt                                                                  >  1:262665/1‑149 (MQ=255)
                                                                             gcagcagGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGt                                                             <  8:29865/149‑1 (MQ=255)
                                                                               agcagGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTgg                                                           <  3:42263/149‑1 (MQ=255)
                                                                                  agGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGtt                                                        <  4:18838/149‑1 (MQ=255)
                                                                                   ggTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGTTg                                                       <  1:58845/149‑1 (MQ=255)
                                                                                          ttCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGCTGGCAGTGGTGAAGTCCAGTGGGTTGACCAGGt                                                >  8:264873/1‑149 (MQ=255)
                                                                                              ttGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGTTGACCAGGTTgg                                             >  5:324387/1‑148 (MQ=255)
                                                                                                gCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGTTGACCAGGTTGGc                                            <  3:106009/147‑1 (MQ=255)
                                                                                                  gTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGTTGACCAGGTTGGCATAg                                        >  5:218359/1‑149 (MQ=255)
                                                                                                   ttCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGTTGACCAGGTTGGCATAGc                                       <  3:195316/149‑1 (MQ=255)
                                                                                                      gCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTc                                                                 <  5:88326/120‑1 (MQ=255)
                                                                                                      gCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTc                                                                 >  6:88326/1‑120 (MQ=255)
                                                                                                      gCCGGTAAACGCCGCCGGGTAGGCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGTTGACCAGGTTGGCATAGCCCg                                    <  3:258397/149‑1 (MQ=255)
                                                                                                       ccGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGTTGACCAGGTTGGCATAGCCCgg                                   <  8:100296/149‑1 (MQ=255)
                                                                                                        cGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGTTGACCAGGTTGGCATAGCCCGGc                                  <  3:307660/149‑1 (MQ=255)
                                                                                                         ggTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGTTGACCAGGTTGGCATAGCCCGGc                                  >  8:288674/1‑148 (MQ=255)
                                                                                                                 ccgccgGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGTTGACCAGGTTGGCATAGCCCGGCAACACCTCg                         <  2:262665/149‑1 (MQ=255)
                                                                                                                 ccgccgGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGTTGACCAGGTTGGCATAGCCCGGCAACACCTCg                         <  6:324387/149‑1 (MQ=255)
                                                                                                                  cgccgGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGTTGACCAGGTTGGCATAGCCCGGCAACACCTCgg                        >  7:289875/1‑149 (MQ=255)
                                                                                                                    ccgGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGTTGACCAGGTTGGCATAGCCCGGCaa                                <  4:176515/139‑1 (MQ=255)
                                                                                                                    ccgGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGTTGACCAGGTTGGCATAGCCCGGCaa                                >  3:176515/1‑139 (MQ=255)
                                                                                                                               agcaccagcaTCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGTTGACCAGGTTGGCATAGCCCGGCAACACCTCGGCCAGTGCCGCCAc           >  2:23672/1‑149 (MQ=255)
                                                                                                                                   tgagcggcgccgcGGGCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGTTGACCAGGTTGGCATAGCCCGGCAACACCTCGGCCAGTGCCGCCACCTGc       >  4:190918/8‑149 (MQ=255)
                                                                                                                                    cagcaTCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGGGAAGGCCAGTGGGTTGACCAGGTTGGCATAGCCCGGCaa                                <  7:276258/123‑1 (MQ=255)
                                                                                                                                     agcggcgccgcGGGCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGTTGACCAGGTTGGCATAGCCCGGCAACACCTCGGCCAGTGCCGCCACCTGcc      <  3:190918/143‑1 (MQ=255)
                                                                                                                                        aTCGCCGCGCCCGCGGCGCCGCTCAGGGGGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGTTGACCAGGTTGGCATAGCCCGGCAACACCTCGGCCAGTGCCGCCACCTGCCCTTg  <  6:13343/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                
AGGCAACAGTTCCGGGAAAGCCGAGGTGACGAAGCCGATCTTGCCGTGGCGTTCAACGGCATCGCAGTACAGCCCCAGCAGCAGGTCGCATTCCTTGCGTTCGCCGGTAAACGCCGCCGGGTAGTCCAGCACCAGCATCGCCGCGCCCGCGGCGCCGCTCAGGGTGTTGTCGAGCATGCGTTGCAGGGCGGCTTCATCGCCCCAGATGGCAGTGGTGAAGTCCAGTGGGTTGACCAGGTTGGCATAGCCCGGCAACACCTCGGCCAGTGCCGCCACCTGCCCTTG  >  NC_002947/4248758‑4249042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑