Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,271,256	G→A	7.5%	A466T (GCC→ACC)	mrdA‑I →	transpeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,271,256	0	G	A	7.5%	155.7 / 2.0	53	A466T (GCC→ACC)	mrdA‑I	transpeptidase
Reads supporting (aligned to +/- strand):  ref base G (24/25);  new base A (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGACATGTTCGAGGAAGCCTCCGGGCTGATGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGACATCGTGCTGCACGACAAGCATGCCTGGGACCAGGTCAGCCAGGGCATGC  >  NC_002947/4271108‑4271403                                                                                                                                                      |                                                                                                                                                    gCTGGACATGTTCGAGGAAGCCTCCGGGCTGATGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAgg                                                                                                                                                     >  4:295951/1‑149 (MQ=255)       cATGTTCGAGGAAGCCTCCGGGCTGATGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGccacca                                                                                                                                               <  3:94566/149‑1 (MQ=255)              gAGGAAGCCTCCGGGCTGATGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCctgct                                                                                                                                        >  7:211358/1‑149 (MQ=255)              gAGGAAGCCTCCGGGCTGATGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCctgct                                                                                                                                        >  7:262047/1‑149 (MQ=255)                ggAAGCCTCCGGGCTGATGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGccacca                                                                                                                                               >  1:223430/1‑140 (MQ=255)                ggAAGCCTCCGGGCTGATGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGccacca                                                                                                                                               <  2:223430/140‑1 (MQ=255)                ggAAGCCTCCGGGCTGATGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGACCACCAGCCTGCTgg                                                                                                                                      >  3:153900/1‑149 (MQ=255)                   aGCCTCCGGGCTGATGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGgcaa                                                                                                                                   <  3:295951/149‑1 (MQ=255)                           ggCTGATGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGc                                                                                                                           >  2:179492/1‑149 (MQ=255)                             cTGATGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCgt                                                                                                                         <  1:148872/149‑1 (MQ=255)                             cTGATGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCgt                                                                                                                         <  5:200775/149‑1 (MQ=255)                              tGATGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCgtg                                                                                                                        >  4:122162/1‑149 (MQ=255)                               gATGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGACCACCAGCCTGCTGgcaagc                                                                                                                                 >  4:31228/1‑139 (MQ=255)                               gATGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGACCACCAGCCTGCTGgcaagc                                                                                                                                 <  3:31228/139‑1 (MQ=255)                                aTGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCACCCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCgtgt                                                                                                                       >  8:170935/1‑149 (MQ=255)                                aTGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGgc                                                                                                                                     >  1:99705/1‑134 (MQ=255)                                aTGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGgc                                                                                                                                     <  2:99705/134‑1 (MQ=255)                                aTGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCgtgt                                                                                                                       <  4:197297/148‑1 (MQ=255)                                aTGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCgtg                                                                                                                        <  3:225179/147‑1 (MQ=255)                                 tGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCACCCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGtgtg                                                                                                                      <  7:170935/149‑1 (MQ=255)                                     cTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGACCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCAcc                                                                                                                 <  4:153900/149‑1 (MQ=255)                                        ggCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCAcggcc                                                                                                              <  5:204762/149‑1 (MQ=255)                                         gCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCAccgccc                                                                                                             <  6:157127/149‑1 (MQ=255)                                         gCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCAccgccc                                                                                                             <  4:212521/149‑1 (MQ=255)                                                       cgcCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGa                                                                                               <  8:211358/149‑1 (MQ=255)                                                            cacGACGGCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTg                                                                                          <  2:245639/149‑1 (MQ=255)                                                             acgacgCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCACCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTgg                                                                                         >  1:268621/1‑149 (MQ=255)                                                                    cAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGTGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGa                                                                                  <  2:246500/149‑1 (MQ=255)                                                                         cTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGc                                                                             <  3:122162/149‑1 (MQ=255)                                                                            gTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCg                                                                           <  8:262047/148‑1 (MQ=255)                                                                            gTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCg                                                                           <  8:83401/148‑1 (MQ=255)                                                                               ccccGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATc                                                                        >  8:120354/1‑148 (MQ=255)                                                                                  cGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGAt                                                                         >  2:75247/1‑144 (MQ=255)                                                                                  cGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGAt                                                                         <  1:75247/144‑1 (MQ=255)                                                                                             tGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCACAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATg                                                         >  1:87711/1‑149 (MQ=255)                                                                                                tCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGccc                                                      >  4:11090/1‑149 (MQ=255)                                                                                                 ccTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGccc                                                      >  3:89279/1‑148 (MQ=255)                                                                                                  cTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGa                                                    <  2:94243/149‑1 (MQ=255)                                                                                                        aTTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGACATCGt                                              >  6:169680/1‑149 (MQ=255)                                                                                                                   ttACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGACATCGTGCTGCACGAc                                    >  6:93597/1‑148 (MQ=255)                                                                                                                            ggTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGACATCGTGCTGCACGACAAGCATGCCt                          <  2:87711/149‑1 (MQ=255)                                                                                                                            ggTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGACATCGTGCTGCACGACAAGCATGCCt                          >  5:202635/1‑149 (MQ=255)                                                                                                                               cA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGACATCGTGCTGTACGACAAGCATGCCTggg                       >  5:243749/1‑149 (MQ=255)                                                                                                                                   cccccTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGACATCGTGCTGCACGACAAGCATGCCTGGGAc                     >  7:171495/1‑148 (MQ=255)                                                                                                                                   cccccTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGACATCGTGCTGCACGACAAGCATGCCTGGGAc                     >  4:213871/1‑148 (MQ=255)                                                                                                                                   cccccTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCACGACATCGTGCTGCACGACAAGCATGCCTGGGAc                     >  8:222297/1‑148 (MQ=255)                                                                                                                                    ccccTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGACATCGTGCTGCACGACAAGCATGCCTGGGACCa                   <  3:213871/149‑1 (MQ=255)                                                                                                                                    ccccTGCAGTTGGCCCAGGCCACCAGCCTGCGGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGACATCGTGCTGCACGACAAGCATGCCTGGGACCa                   <  4:26445/149‑1 (MQ=255)                                                                                                                                        tGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGACATCGTGCTGCACGACAAGCATGCCTGGGACCAGGTc               >  3:227463/1‑149 (MQ=255)                                                                                                                                            gTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGACATCGTGCTGCACGACAAGCATGCCTg                         >  1:216350/1‑135 (MQ=255)                                                                                                                                            gTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGACATCGTGCTGCACGACAAGCATGCCTg                         <  2:216350/135‑1 (MQ=255)                                                                                                                                                gCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGACATCGTGCTGTACGACAAGCATGCCTGGGACCAGGTCAGCCAgg        <  6:243749/148‑1 (MQ=255)                                                                                                                                                 cccAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGACATCGTGCTGCACGACAAGCATGCCTGGGACCAGGTCAGCCAggg       >  6:91951/1‑148 (MQ=255)                                                                                                                                                   cAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGACATCGTGCTGCACGACAAGCATGCCTGGGACCAGGTCAGCCAGGGCAt    >  4:195726/1‑149 (MQ=255)                                                                                                                                                     ggCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGACATCGTGCTGCACGACAAGCATGCCTGGGACCAGGTCAGCCAGGGCATGc  <  5:91951/149‑1 (MQ=255)                                                                                                                                                     ggCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGACATCGTGCTGCACGACAAGCATGCCTGGGACCAGGTCAGCCAGGGCATGc  <  4:89279/149‑1 (MQ=255)                                                                                                                                                      gCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGACATCGTGCTGCACGACAAGCATGCCTGGGACCAGGTCAGCCAGGGCATg   >  2:119006/1‑147 (MQ=255)                                                                                                                                                      |                                                                                                                                                    GCTGGACATGTTCGAGGAAGCCTCCGGGCTGATGCCCTCGGCCGAGTGGAAGCGCGCCACACGACGCCAGGCCTGGTTCCCCGGCGAGACGCTGATCCTTGGCATTGGCCAGGGTTACATGCAGGTCA‑CCCCCCTGCAGTTGGCCCAGGCCACCAGCCTGCTGGCAAGCAAGGGCGTGTGGCACCGCCCGCACCTGGCCATGACCGTGGGCGGCGATACGCCGATCGACCCAAACCCCATGCCCGACATCGTGCTGCACGACAAGCATGCCTGGGACCAGGTCAGCCAGGGCATGC  >  NC_002947/4271108‑4271403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑