Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,299,657	T→C	6.4%	G96G (GGT→GGC)	PP_3772 →	repressor protein‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,299,657	0	T	C	6.4%	180.4 / 2.0	62	G96G (GGT→GGC)	PP_3772	repressor protein‑like protein
Reads supporting (aligned to +/- strand):  ref base T (36/22);  new base C (2/2);  total (38/24)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

ACACCTTAGCCAGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGCCCC  >  NC_002947/4299510‑4299804                                                                                                                                                    |                                                                                                                                                           acacCTTAGCCAGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTCGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGt                                                                                                                                                            <  4:226713/148‑1 (MQ=255) acacCTTAGCCAGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTCGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGt                                                                                                                                                            >  3:226713/1‑148 (MQ=255)   acCTTAGCCAGATCCTTTCCGACCAGTCGGTGAATACCCTGGGCGAGGACGTGCCCCGCCGCATCGAGGGCAGCGAAGGCTTGCGACGGGGCTGGTTCGACGCGCTGTGGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGac                                                                                                                                                         <  7:225973/149‑1 (MQ=255)    ccTTAGCCAGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGac                                                                                                                                                         >  4:22697/1‑148 (MQ=255)          ccAGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGc                                                                                                                                                  >  1:178233/1‑149 (MQ=255)            aGATACTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGAATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCGGACGAGCCAGGTGACAACAAGctc                                                                                                                                                >  8:142676/1‑149 (MQ=255)                  tttCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTg                                                                                                                                         >  2:26714/1‑147 (MQ=255)                  tttCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTg                                                                                                                                         >  1:190921/1‑147 (MQ=255)                              ccGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTc                                                                                                                           <  3:22697/149‑1 (MQ=255)                                          tGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAACCAGATGCTg                                                                                                               >  3:311147/1‑149 (MQ=255)                                          tGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTg                                                                                                               >  3:306256/1‑149 (MQ=255)                                           gggCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTgg                                                                                                              >  4:67253/1‑149 (MQ=255)                                                   aCGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTg                                                                                                                              <  3:58333/125‑1 (MQ=255)                                                   aCGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTg                                                                                                                              >  4:58333/1‑125 (MQ=255)                                                     gTGGCCCGCCGCATCGAGGGCATCGAAGCCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑Cgg                                                                                                   >  1:185414/1‑149 (MQ=255)                                                      tGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGCGACAACACGCTCT‑‑‑CTGCAGCCGACCGGGTCAAACAGATGCTGGCCCGACG‑Cgg                                                                                                   >  6:305906/1‑148 (MQ=255)                                                      tGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGCGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑Cgg                                                                                                   >  6:310676/1‑148 (MQ=255)                                                        gtccgccgCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCa                                                                                                 >  6:274689/3‑148 (MQ=255)                                                        gCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGCGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCaa                                                                                                <  5:310676/149‑1 (MQ=255)                                                        gCCCGCCGCATCGAGGGCATCGAAGGCGTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGCGACAACAAGCTCG‑‑‑CTGCAGCCGACCTGGGCAAACAGATGCTGGCCCGAAG‑CGGCaa                                                                                                <  5:305906/149‑1 (MQ=255)                                                         cccGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCaaa                                                                                               <  2:190921/149‑1 (MQ=255)                                                          ccgccgCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAg                                                                                              >  5:278059/1‑149 (MQ=255)                                                          ccgccgCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAg                                                                                              <  2:178233/149‑1 (MQ=255)                                                                 aTCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGt                                                                                                                                                            <  3:278225/84‑1 (MQ=255)                                                                 aTCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGt                                                                                                                                                            >  4:278225/1‑84 (MQ=255)                                                                     aGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCgaa                                                                                   <  6:278059/149‑1 (MQ=255)                                                                       ggCATCGAAGGCTTGCCACGGGGCTGGTTAGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGCTG‑CGGCAAAGGTATCTCCgaag                                                                                  >  6:236448/1‑148 (MQ=255)                                                                          aTCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTg                                                                                                               <  4:120156/117‑1 (MQ=255)                                                                          aTCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTg                                                                                                               >  3:120156/1‑117 (MQ=255)                                                                             gAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCg                                                                           >  5:66681/1‑149 (MQ=255)                                                                              aaGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCgg                                                                          >  5:211223/1‑149 (MQ=255)                                                                                   ttGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAg                                                                  >  1:130183/1‑149 (MQ=255)                                                                                       cACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGc                                                                                                             >  4:106967/1‑106 (MQ=255)                                                                                       cACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGc                                                                                                             <  3:106967/106‑1 (MQ=255)                                                                                       cACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGgctg                                                              >  8:139885/1‑149 (MQ=255)                                                                                        aCGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGgctgc                                                             >  6:99430/1‑149 (MQ=255)                                                                                        aCGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAACGTATCTCCGAAGAAACCCGGCA‑‑‑AAGgctgc                                                             <  1:26714/149‑1 (MQ=255)                                                                                            ggCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCaaa                                                                                                                        >  5:120009/1‑90 (MQ=255)                                                                                            ggCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCaaa                                                                                                                        <  8:31155/90‑1 (MQ=255)                                                                                            ggCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCaaa                                                                                                                        >  7:31155/1‑90 (MQ=255)                                                                                            ggCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCaaa                                                                                                                        <  6:120009/90‑1 (MQ=255)                                                                                             gCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTgg                                                          >  3:165729/1‑147 (MQ=255)                                                                                               tGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCTGGGCTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTgg                                                          >  1:267503/1‑148 (MQ=255)                                                                                               tGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAgc                                                      >  2:163184/1‑149 (MQ=255)                                                                                                          gcTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGc                                           >  2:98032/1‑149 (MQ=255)                                                                                                          gcTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGc                                           >  6:57333/1‑149 (MQ=255)                                                                                                           cTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAGACGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGc                                           <  1:255501/149‑1 (MQ=255)                                                                                                                gCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGCCTg                                        >  5:26432/1‑146 (MQ=255)                                                                                                                gCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGCCTGCa                                      <  1:163184/148‑1 (MQ=255)                                                                                                                 ccAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGCCTg                                        >  1:58032/1‑145 (MQ=255)                                                                                                                  cAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGAt                                   >  8:53323/1‑149 (MQ=255)                                                                                                                    ggCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGc                                 <  7:139885/149‑1 (MQ=255)                                                                                                                       gAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCAc                              >  8:211248/1‑149 (MQ=255)                                                                                                                           acGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCAGGTGAGGCTGCTGGCAGCGGCGCAAGAGCATGCAGATGCCACg                             >  1:110819/1‑149 (MQ=255)                                                                                                                             gCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAaga                                             <  4:133052/128‑1 (MQ=255)                                                                                                                             gCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAaga                                             >  3:133052/1‑128 (MQ=255)                                                                                                                              cacaAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTGCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGctgctg                       <  5:236448/149‑1 (MQ=255)                                                                                                                                caAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGa                     <  5:99430/149‑1 (MQ=255)                                                                                                                                caAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGa                     <  6:26432/149‑1 (MQ=255)                                                                                                                                     ggCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGcc                 >  6:123972/1‑148 (MQ=255)                                                                                                                                            gAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGa               >  2:93133/1‑143 (MQ=255)                                                                                                                                            gAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGa               <  1:93133/143‑1 (MQ=255)                                                                                                                                             aGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCtt        <  5:274689/149‑1 (MQ=255)                                                                                                                                              gCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTc       <  6:66681/149‑1 (MQ=255)                                                                                                                                              gCCAGGTGACAACAAGCTCT‑‑‑CAGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTc       <  7:142676/149‑1 (MQ=255)                                                                                                                                                   gTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGcccc  >  2:22169/1‑149 (MQ=255)                                                                                                                                                    tGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGc             >  5:201120/1‑137 (MQ=255)                                                                                                                                                    tGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGc             <  6:201120/137‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                           ACACCTTAGCCAGATCCTTTCCGACAAGACCGTGAAAAACCTGGGCGATGACGTGGCCCGCCGCATCGAGGGCATCGAAGGCTTGCCACGGGGCTGGTTCGACGCGCTGTCGCCAGGCGAACACGCACAAGCGGCTGTCGAGCCAGGTGACAACAAGCTCT‑‑‑CTGCAGCCGACCTGGTCAAACAGATGCTGGCCCGAAG‑CGGCAAAGGTATCTCCGAAGAAACCCGGCA‑‑‑AAGGCTGCTGGCAGCGGCGCAAGAGCCTGCAGATGCCACGCTGCTGGAGGCCGAGCCGCTTCGCCCC  >  NC_002947/4299510‑4299804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑