Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,305,611	T→C	5.9%	A148A (GCA→GCG)	PP_3777 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,305,611	0	T	C	5.9%	197.1 / 2.1	68	A148A (GCA→GCG)	PP_3777	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (33/31);  new base C (2/2);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

CAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGTTGACCAGCTCATT  >  NC_002947/4305463‑4305757                                                                                                                                                      |                                                                                                                                                    cAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGt                                                                                                                                                     <  3:175489/149‑1 (MQ=255)    tGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTCTGGGCAGCCTCGATGCGCTGTAGTGc                                                                                                                                                   <  1:172778/149‑1 (MQ=255)        gACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATc                                                                                                                                                >  7:231093/1‑147 (MQ=255)          ccAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAgg                                                                                                                                            <  5:21930/149‑1 (MQ=255)          ccAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAg                                                                                                                                             >  4:231819/1‑148 (MQ=255)           cAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCACTACAGCGCATCGAg                                                                                                                                             <  4:211002/147‑1 (MQ=255)           cAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCACTACAGCGCATCGAg                                                                                                                                             >  3:211002/1‑147 (MQ=255)            aaCGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑aa                                                                                                                                         <  7:94326/149‑1 (MQ=255)             aCGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAg                                                                                                                                        >  5:33683/1‑149 (MQ=255)                     aGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATcc                                                                                                                                  >  8:244408/1‑147 (MQ=255)                         cGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGc                                                                                                                            <  5:102472/149‑1 (MQ=255)                          gatgatCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑aa                                                                                                                                         <  2:116844/135‑1 (MQ=255)                          gatgatCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑aa                                                                                                                                         >  1:116844/1‑135 (MQ=255)                                  gCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCAc                                                                                                                   >  8:262542/1‑149 (MQ=255)                                   cTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCAcc                                                                                                                  <  3:153217/149‑1 (MQ=255)                                    ttGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCAccc                                                                                                                 >  7:158159/1‑149 (MQ=255)                                      gCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCt                                                                                                                <  7:79684/148‑1 (MQ=255)                                        cTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGc                                                                                                             >  3:26678/1‑149 (MQ=255)                                        cTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGc                                                                                                             <  3:75063/149‑1 (MQ=255)                                        cTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGc                                                                                                             >  5:187766/1‑149 (MQ=255)                                        cTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGc                                                                                                             <  3:231819/149‑1 (MQ=255)                                         tCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑CGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCg                                                                                                            >  2:71196/1‑149 (MQ=255)                                          ccGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCg                                                                                                            >  8:28885/1‑148 (MQ=255)                                                ttCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGa                                                                                                      >  8:23321/1‑148 (MQ=255)                                                  cAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGaa                                                                                                     <  4:149108/147‑1 (MQ=255)                                                  cAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGaa                                                                                                     <  4:154107/147‑1 (MQ=255)                                                  cAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGaa                                                                                                     <  7:262542/147‑1 (MQ=255)                                                     gTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGa                                                                                                >  2:229002/1‑149 (MQ=255)                                                      tAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGAt                                                                                               <  8:158159/149‑1 (MQ=255)                                                       aGCTCGGCACCCGTTCTAAAAACCTGCGCAAGGTCCGCTCGCCATTGATCATCTCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGTAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGa                                                                                                <  1:229002/148‑1 (MQ=255)                                                          tCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGa                                                                                                <  6:269582/144‑1 (MQ=255)                                                          tCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGa                                                                                                >  5:269582/1‑144 (MQ=255)                                                               aCCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTa                                                                                      >  7:9627/1‑149 (MQ=255)                                                                cccGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTAt                                                                                     <  6:187766/149‑1 (MQ=255)                                                                cccGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑CGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTAt                                                                                     <  1:71196/149‑1 (MQ=255)                                                                 ccGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATc                                                                                    >  5:283329/1‑149 (MQ=255)                                                                      cGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTc                                                                                <  6:253569/148‑1 (MQ=255)                                                                          aaaCCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTACAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTCCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTg                                                                           >  4:320475/1‑149 (MQ=255)                                                                            aCCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGcc                                                                         >  4:14595/1‑149 (MQ=255)                                                                                     aGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAtt                                                                <  7:23321/149‑1 (MQ=255)                                                                                       gTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGc                                                              >  7:105250/1‑149 (MQ=255)                                                                                          cGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGt                                                            <  7:244408/148‑1 (MQ=255)                                                                                          cGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGt                                                            <  6:33683/148‑1 (MQ=255)                                                                                           gCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCg                                                          <  7:28885/149‑1 (MQ=255)                                                                                                ccATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGaa                                                        >  5:133108/1‑146 (MQ=255)                                                                                                  aTAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGa                                                    >  4:126613/1‑148 (MQ=255)                                                                                                        cATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACa                                                      <  2:241215/140‑1 (MQ=255)                                                                                                        cATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACa                                                      >  1:241215/1‑140 (MQ=255)                                                                                                               tCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGa                                                    >  8:218006/1‑135 (MQ=255)                                                                                                               tCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGa                                                    <  7:218006/135‑1 (MQ=255)                                                                                                                  aGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTc                                   >  4:119790/1‑149 (MQ=255)                                                                                                                  aGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTc                                   >  4:289808/1‑149 (MQ=255)                                                                                                                       ccgCCCT‑TGGGCAGCCTCGATGCGCGGTAGTGCATCGAGG‑AAGCCATCCGCGGGCGTCTGCCACCCTGGCGGATTGAATAGGACCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACCGATCTCCCTGTACGAATTCGATa                               >  5:52604/3‑148 (MQ=255)                                                                                                                       cAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGAACACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATa                               >  5:52601/1‑148 (MQ=255)                                                                                                                        aGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACa                             <  3:119790/149‑1 (MQ=255)                                                                                                                          cccT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAg                            >  6:32069/1‑148 (MQ=255)                                                                                                                           gccgtGGTCATCCTCGATGCGCTGTATTGCCTAGAGG‑AAGCCTTCCGAGGGCTTCTGCCACCCTTTCTGATTGAATATGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAgg                           <  4:26678/145‑1 (MQ=255)                                                                                                                           ccT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGTCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGa                          >  7:267797/1‑149 (MQ=255)                                                                                                                           ccT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTc                                                           >  4:171056/1‑116 (MQ=255)                                                                                                                           ccT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTc                                                           <  3:171056/116‑1 (MQ=255)                                                                                                                           ccT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTAGGAATTCGATACAGGa                          >  4:36896/1‑149 (MQ=255)                                                                                                                                 ggCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGc                     <  3:289808/149‑1 (MQ=255)                                                                                                                                   cAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGcc                    >  4:274113/1‑148 (MQ=255)                                                                                                                                        tCGATGCACTACAGCGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGAt                         >  1:147779/1‑138 (MQ=255)                                                                                                                                        tCGATGCACTACAGCGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGAt                         <  2:147779/138‑1 (MQ=255)                                                                                                                                           aTGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGTTGAc           <  3:320475/149‑1 (MQ=255)                                                                                                                                                  gTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGTTGACCAGCTCa    >  3:252022/1‑149 (MQ=255)                                                                                                                                                   tAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGTTGACCAGCTCAt   <  3:126613/149‑1 (MQ=255)                                                                                                                                                     gTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGTTGACCAGCTCAtt  <  8:231093/148‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                    CAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCT‑TGGGCAGCCTCGATGCGCTGTAGTGCATCGAGG‑AAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTACGAATTCGATACAGGATTCGCCCAGGTTGACCAGCTCATT  >  NC_002947/4305463‑4305757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑