Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,452,687 G→A 100% W764* (TGG→TAG)  nicB → nicotinate dehydrogenase subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,452,6870GA100.0% 155.5 / NA 47W764* (TGG→TAG) nicBnicotinate dehydrogenase subunit B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/24);  total (23/24)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCAATGCCATTTTCGACGCCACCGGCATTCGTTTTCGCGAGCTGCCGATCACAGCCGAACGCGTGCGTGCTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTGGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCGGTAACGCCACCTGGTGTCGGCAGCTGGAGCGCCGCCATGCTCGAGCGCGGGCGCCAGGTG  >  NC_002947/4452546‑4452832
                                                                                                                                             |                                                                                                                                                 
ccAATGCCATTTTCGACGCCACCGGCATTCGTTTTCGCGAGCTGCCGATCACAGCCGAACGCGTGCGTGCTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTc                                                                                                                                            <  7:211656/149‑1 (MQ=255)
              gACGCCACCGGCATTCGTTTTCGCGAGCTGCCGATCACAGCCGAACGCGTGCGTGTTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCgg                                                                                                                              <  3:195932/149‑1 (MQ=255)
                   cACCGGCATTCGTTTTCGCGAGCTGCCGATCACAGCCGAACGCGTGCGTGCTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGgtgt                                                                                                                         >  4:61460/1‑149 (MQ=255)
                      cGGCATTCGTTTTCGCGAGCTGCCGATCACAGCCGAACGCGTGCGTGCTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCTCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTggaccgg                                                                                                                             >  3:13908/1‑137 (MQ=255)
                      cGGCATTCGTTTTCGCGAGCTGCCGATCACAGCCGAACGCGTGCGTGCTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCg                                                                                                                      >  5:21063/1‑149 (MQ=255)
                      cGGCATTCGTTTTCGCGAGCTGCCGATCACAGCCGAACGCGTGCGTGCTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCg                                                                                                                      >  6:302163/1‑149 (MQ=255)
                          aTTCGTTTTCGCGAGCTGCCGATCACAGCCGAACGCGTGCGTGCTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGgcgc                                                                                                                  >  6:160697/1‑149 (MQ=255)
                               ttttCGCGCTCTGACGATCACAGCCGAACGCGTGCGTGCTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCAt                                                                                                             <  8:131712/149‑1 (MQ=255)
                                 ttCGCGAGCTGCCGATCACAGCCGAACGCGTGCGTGCTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCAtt                                                                                                            <  5:302163/148‑1 (MQ=255)
                                   cgcgAGCTGCCGATCACAGCCGAACGCGTGCGTGCTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTgg                                                                                                          <  8:182726/148‑1 (MQ=255)
                                   cgcgAGCTGCCGATCACAGCCGAACGCGTGCGTGCTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTgg                                                                                                          <  6:276487/148‑1 (MQ=255)
                                   cgcgAGCTGCCGATCACAGCCGAACGCGTGCGTGCTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTgg                                                                                                          <  3:61460/148‑1 (MQ=255)
                                    gcgAGCTGCCGATCACAGCCGAACGCGTGCGTGCTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGc                                                                                                        <  6:34843/149‑1 (MQ=255)
                                    gcgAGCTGCCGATCACAGCCGAACGCGTGCGTGCTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGACTCGCTGGCCGGGGTGTTCGGCGCCGCATTggg                                                                                                         <  2:52701/148‑1 (MQ=255)
                                     cgAGCTGCCGATCACAGCCGAACGCGTGCGTGCTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGcgcc                                                                                                                 <  3:73176/139‑1 (MQ=255)
                                     cgAGCTGCCGATCACAGCCGAACGCGTGCGTGCTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGcgcc                                                                                                                 >  4:73176/1‑139 (MQ=255)
                                        gCTGCCGATCACAGCCGAACGCGTGCGTGCTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAACCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGc                                                                                                    <  5:314603/149‑1 (MQ=255)
                                              gATCACAGCCGAACGCGTGCGTGCTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCa                                                                                              >  4:267607/1‑149 (MQ=255)
                                                         aaCGCGTGCGTGCTGCGCTCGGTGGCGAAGTCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGGATTGGGCATGCTTGCCACCGCCTTGccc                                                                                   <  1:37705/149‑1 (MQ=255)
                                                              gtgcgtgcTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGgcg                                                                              >  3:315017/1‑149 (MQ=255)
                                                                   tgctgcGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGcc                                                                          >  2:232256/1‑148 (MQ=255)
                                                                    gctgcGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCg                                                                         >  4:54727/1‑148 (MQ=255)
                                                                      tgcGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGaaa                                                                      <  2:256274/149‑1 (MQ=255)
                                                                              gTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCg                                                              >  5:155383/1‑149 (MQ=255)
                                                                                 gCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCGGt                                                            >  2:90555/1‑148 (MQ=255)
                                                                                   gAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGcc                                                                          >  6:9395/1‑132 (MQ=255)
                                                                                   gAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCGGTAAc                                                         <  3:267607/149‑1 (MQ=255)
                                                                                       gCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCGGTAACGCCa                                                     <  6:155383/149‑1 (MQ=255)
                                                                                         cAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGcccc                                                               >  1:247035/1‑137 (MQ=255)
                                                                                         cAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGcccc                                                               <  2:247035/137‑1 (MQ=255)
                                                                                           aGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCGGTAACGCCACCTg                                                 <  1:90555/149‑1 (MQ=255)
                                                                                            gggCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCGGTAACGCCACCTgg                                                >  5:323981/1‑149 (MQ=255)
                                                                                            gggCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCGGTAACGCCACCTgg                                                >  8:292574/1‑149 (MQ=255)
                                                                                                  gATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTAGCCCTGGCGCGCCGAAATCGCCCCGGTAACGCCACCTGGTGTCgg                                          <  1:232256/149‑1 (MQ=255)
                                                                                                      cgcCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCGGTAACGCCACCTGGTGTCGGCAGc                                      <  7:292574/149‑1 (MQ=255)
                                                                                                       gcCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCGGTAACGCCACCTGGTGTCGGCAGCt                                     >  8:61453/1‑149 (MQ=255)
                                                                                                       gcCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCGGTAACGCCACCTGGTGTCGGCAGCt                                     <  3:54727/149‑1 (MQ=255)
                                                                                                             gcCAGCGCAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCGGTAACGCCACCTGGTGTCGGCAGCTGGAgcg                               >  5:214085/1‑149 (MQ=255)
                                                                                                                  cgcAGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCGGTAACGCCACCTGGTGTCGGCAGCTGGAGcgccgc                           >  7:116637/1‑148 (MQ=255)
                                                                                                                     aGCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCGGTAACGCCACCTGGTGTCGGCAGCTGGAGCGCCGCCATg                       >  6:134980/1‑149 (MQ=255)
                                                                                                                      gCCGTCAACCAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCGGTAACGCCACCTGGTGTCGGCAGCTGGAGCGCCGCCATGc                      <  6:323981/149‑1 (MQ=255)
                                                                                                                              ccAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGTCCCGGTAACGCCACCTGGTGTCGGCAGCTGGAGCGCCGCCATGCTCGAgcgc              >  4:279402/1‑149 (MQ=255)
                                                                                                                              ccAAGCGTTCGAAGTAGTGGTTAGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGACCTGGCGCGCCGAAATCGCCCCGGTAACGCCACCTGGTGTCGGCAGCTGGAGCGCCGCCATGCTCGAgcgc              >  3:91738/1‑149 (MQ=255)
                                                                                                                               cAAGCGTTCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCGGTAACGCCACCt                                                  <  2:283142/112‑1 (MQ=255)
                                                                                                                                  gCGTTCGAAGTAGTGGTGCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCGGTAACGCCACCTGGTGTCGGCAGCTGGAGCGCCGCCATGCTCGAGCGCGGgc          <  7:243809/149‑1 (MQ=255)
                                                                                                                                     ttCGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCGGTAACGCCACCTGGTGTCGGCAGCTGGAGCGCCGCCATGCTCGAGCGCGGGCGcc       >  4:71967/1‑149 (MQ=255)
                                                                                                                                       cGAAGTAGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCGGTAACGCCACCTGGTGTCGGCAGCTGGAGCGCCGCCATGCTCGAGCGCGGGCGCCa      <  6:214085/148‑1 (MQ=255)
                                                                                                                                          agtagtGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCGGTAACGCCACCTGGTGTCGGCAGCTGGAGCGCCGCCATGCTCGAGCGCGGGCGCCAGGTg  >  5:190256/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
CCAATGCCATTTTCGACGCCACCGGCATTCGTTTTCGCGAGCTGCCGATCACAGCCGAACGCGTGCGTGCTGCGCTCGGTGGCGAAGGCCAAGGGCCAGATGCGCCCGCGCCAGCGCAGCCGTCAACCAAGCGTTCGAAGTGGTGGTTCGGCTCGCTGGCCGGGGTGTTCGGCGCCGCATTGGGCATGCTTGCCACCGCCTTGCCCTGGCGCGCCGAAATCGCCCCGGTAACGCCACCTGGTGTCGGCAGCTGGAGCGCCGCCATGCTCGAGCGCGGGCGCCAGGTG  >  NC_002947/4452546‑4452832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑