Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,496,668 A→G 6.6% intergenic (+18/+34) PP_3987 → / ← PP_5651 site‑specific recombinase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,496,6680AG 6.6% 172.3 / 2.4 60intergenic (+18/+34)PP_3987/PP_5651site‑specific recombinase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (25/31);  new base G (2/2);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

ATACGCCCCAAGGCGGCAAGCGAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCTTTGGACCTTGCCA  >  NC_002947/4496522‑4496811
                                                                                                                                                  |                                                                                                                                               
aTACGCCCCAAGGCGGCAAGCGAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACg                                                                                                                                               >  3:122539/1‑149 (MQ=255)
           ggcggcAAGCGAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGAGCGTACCCCGCttc                                                                                                                                    <  1:32451/149‑2 (MQ=255)
             cggcAAGCGAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGa                                                                                                                                  <  8:101737/149‑1 (MQ=255)
             cggcAAGCGAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGa                                                                                                                                  <  4:122539/149‑1 (MQ=255)
                     gAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAAtt                                                                                                                          <  7:127570/149‑1 (MQ=255)
                       aaTCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTc                                                                                                                         <  1:306463/148‑1 (MQ=255)
                        aTCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTcac                                                                                                                       <  3:92422/149‑1 (MQ=255)
                           ggCGATTTGGGTCCAGCCAGGCGCTTATTGGGGCCCACTTGCAAGGCGATCCACCATACCGTAAATATACCTGGATGTGAAATCGTTCACCCCACACGATACGTAAATGGAACCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAAc                                                                                                                    <  2:230760/149‑1 (MQ=255)
                           ggCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATCTAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACaa                                                                                                                     <  8:265461/148‑1 (MQ=255)
                              gATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGa                                                                                                                 >  5:321682/1‑149 (MQ=255)
                               aTTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGa                                                                                                                 >  7:278303/1‑148 (MQ=255)
                                   gggTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTa                                                                                                             <  6:31350/148‑1 (MQ=255)
                                   gggTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTAt                                                                                                            >  7:41297/1‑149 (MQ=255)
                                      tCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTAtt                                                                                                           >  1:41935/1‑147 (MQ=255)
                                      tCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGa                                                                                                         <  8:41297/149‑1 (MQ=255)
                                          gCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCta                                                                                                     >  2:304848/1‑149 (MQ=255)
                                            aaGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCtata                                                                                                   <  2:216832/149‑1 (MQ=255)
                                                     aTTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGAt                                                                                                                <  6:97086/127‑1 (MQ=255)
                                                     aTTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGAt                                                                                                                >  5:97086/1‑127 (MQ=255)
                                                        gggTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATc                                                                                        <  2:259876/148‑1 (MQ=255)
                                                           tCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTAtt                                                                                                           >  6:28238/1‑126 (MQ=255)
                                                           tCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTAtt                                                                                                           <  5:28238/126‑1 (MQ=255)
                                                            cacaCTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCt                                                                                   >  5:5790/1‑149 (MQ=255)
                                                             acacTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCt                                                                                   <  6:321682/148‑1 (MQ=255)
                                                               acTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAg                                                                                >  4:273505/1‑149 (MQ=255)
                                                                 tGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCt                                                                              >  6:336525/1‑149 (MQ=255)
                                                                 tGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCt                                                                              >  7:180092/1‑149 (MQ=255)
                                                                   aCAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCt                                                                              <  2:195478/147‑1 (MQ=255)
                                                                   aCAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTtg                                                                            >  4:312033/1‑149 (MQ=255)
                                                                    cAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTtgc                                                                           <  3:177277/149‑1 (MQ=255)
                                                                     aaGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTa                                                                                 >  6:134142/1‑142 (MQ=255)
                                                                     aaGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTa                                                                                 <  5:134142/142‑1 (MQ=255)
                                                                       gcgcATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTtgctgc                                                                        >  6:52907/1‑149 (MQ=255)
                                                                              acacaAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCa                                                                 >  6:226842/1‑149 (MQ=255)
                                                                                      ggTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAAt                                                                                     <  3:213202/121‑1 (MQ=255)
                                                                                      ggTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAAt                                                                                     >  4:213202/1‑121 (MQ=255)
                                                                                          tataGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAAtcatca                                                      <  5:272525/148‑1 (MQ=255)
                                                                                          tataGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAAtcatca                                                      >  8:217234/1‑148 (MQ=255)
                                                                                            taGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGTACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATa                                                    <  7:49786/148‑1 (MQ=255)
                                                                                            taGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAg                                                   >  1:186713/1‑149 (MQ=255)
                                                                                            taGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAg                                                   <  5:336525/149‑1 (MQ=255)
                                                                                                 gAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTc                                              <  2:41935/149‑1 (MQ=255)
                                                                                                      ggTGAAATCGTACAGCAAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCgaga                                          <  7:217234/148‑1 (MQ=255)
                                                                                                             tCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATAcc                                   >  6:89750/1‑148 (MQ=255)
                                                                                                             tCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCt                                  >  3:98017/1‑149 (MQ=255)
                                                                                                                     ccAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGcc                          >  2:75155/1‑149 (MQ=255)
                                                                                                                        acacGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGa                       <  3:312033/149‑1 (MQ=255)
                                                                                                                        acacGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGa                       <  3:273505/149‑1 (MQ=255)
                                                                                                                         cacGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAg                      <  8:180092/149‑1 (MQ=255)
                                                                                                                           cGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTa                    <  6:5790/149‑1 (MQ=255)
                                                                                                                            gATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTTATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGctct                                                                     <  6:145481/99‑1 (MQ=255)
                                                                                                                            gATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGctct                                                                     >  5:145481/1‑99 (MQ=255)
                                                                                                                                 gTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCa                                                                                       <  1:281588/76‑1 (MQ=255)
                                                                                                                                 gTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGGATCa                                                                                       >  2:281588/1‑76 (MQ=255)
                                                                                                                                    aTTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCTTTg            <  5:226842/148‑1 (MQ=255)
                                                                                                                                      tcaaaGCGGGGAGCGTACCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCttt             >  4:169973/4‑145 (MQ=255)
                                                                                                                                      tcaaaGCGGGGAGCGTACCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCttt             <  3:169973/142‑1 (MQ=255)
                                                                                                                                      tcaaaGCGGGGAGCGTACCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCTTTGGAc         >  8:96067/4‑149 (MQ=255)
                                                                                                                                             ggggTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCTTTGGACCTTGCCa  <  5:52907/149‑1 (MQ=255)
                                                                                                                                              gggTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTCCCACCCAATCATCAAAGGTTTCGAGAGAATACCACGCACGCCTGAGTAGCTCTTTGGACCTTGc    >  7:215317/1‑146 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
ATACGCCCCAAGGCGGCAAGCGAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCTTTGGACCTTGCCA  >  NC_002947/4496522‑4496811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑