| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,496,672 | T→A | 6.6% | intergenic (+22/+30) | PP_3987 → / ← PP_5651 | site‑specific recombinase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,496,672 | 0 | T | A | 6.6% | 182.8 / 2.7 | 61 | intergenic (+22/+30) | PP_3987/PP_5651 | site‑specific recombinase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (25/32); new base A (2/2); total (27/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
GGCGGCAAGCGAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCTTTGGACCTTGCCAGAGCTAGT > NC_002947/4496533‑4496819 | ggcggcAAGCGAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGAGCGTACCCCGCttc < 1:32451/149‑2 (MQ=255) cggcAAGCGAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGa < 8:101737/149‑1 (MQ=255) cggcAAGCGAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGa < 4:122539/149‑1 (MQ=255) gAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAAtt < 7:127570/149‑1 (MQ=255) aaTCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTc < 1:306463/148‑1 (MQ=255) aTCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTcac < 3:92422/149‑1 (MQ=255) ggCGATTTGGGTCCAGCCAGGCGCTTATTGGGGCCCACTTGCAAGGCGATCCACCATACCGTAAATATACCTGGATGTGAAATCGTTCACCCCACACGATACGTAAATGGAACCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAAc < 2:230760/149‑1 (MQ=255) ggCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATCTAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACaa < 8:265461/148‑1 (MQ=255) gATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGa > 5:321682/1‑149 (MQ=255) aTTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGa > 7:278303/1‑148 (MQ=255) gggTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTa < 6:31350/148‑1 (MQ=255) gggTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTAt > 7:41297/1‑149 (MQ=255) tCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTAtt > 1:41935/1‑147 (MQ=255) tCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGa < 8:41297/149‑1 (MQ=255) gCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCta > 2:304848/1‑149 (MQ=255) aaGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCtata < 2:216832/149‑1 (MQ=255) aTTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGAt > 5:97086/1‑127 (MQ=255) aTTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGAt < 6:97086/127‑1 (MQ=255) gggTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATc < 2:259876/148‑1 (MQ=255) tCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTAtt > 6:28238/1‑126 (MQ=255) tCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTAtt < 5:28238/126‑1 (MQ=255) cacaCTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCt > 5:5790/1‑149 (MQ=255) acacTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCt < 6:321682/148‑1 (MQ=255) acTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAg > 4:273505/1‑149 (MQ=255) tGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCt > 6:336525/1‑149 (MQ=255) tGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCt > 7:180092/1‑149 (MQ=255) aCAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCt < 2:195478/147‑1 (MQ=255) aCAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTtg > 4:312033/1‑149 (MQ=255) cAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTtgc < 3:177277/149‑1 (MQ=255) aaGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTa < 5:134142/142‑1 (MQ=255) aaGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTa > 6:134142/1‑142 (MQ=255) gcgcATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTtgctgc > 6:52907/1‑149 (MQ=255) acacaAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCa > 6:226842/1‑149 (MQ=255) ggTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAAt > 4:213202/1‑121 (MQ=255) ggTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAAt < 3:213202/121‑1 (MQ=255) tataGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAAtcatca < 5:272525/148‑1 (MQ=255) tataGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAAtcatca > 8:217234/1‑148 (MQ=255) taGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGTACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATa < 7:49786/148‑1 (MQ=255) taGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAg > 1:186713/1‑149 (MQ=255) taGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAg < 5:336525/149‑1 (MQ=255) gAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTc < 2:41935/149‑1 (MQ=255) ggTGAAATCGTACAGCAAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCgaga < 7:217234/148‑1 (MQ=255) tCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATAcc > 6:89750/1‑148 (MQ=255) tCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCt > 3:98017/1‑149 (MQ=255) ccAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGcc > 2:75155/1‑149 (MQ=255) acacGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGa < 3:312033/149‑1 (MQ=255) acacGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGa < 3:273505/149‑1 (MQ=255) cacGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAg < 8:180092/149‑1 (MQ=255) cGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTa < 6:5790/149‑1 (MQ=255) gATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTTATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGctct < 6:145481/99‑1 (MQ=255) gATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGctct > 5:145481/1‑99 (MQ=255) gTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCa < 1:281588/76‑1 (MQ=255) gTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGGATCa > 2:281588/1‑76 (MQ=255) aTTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCTTTg < 5:226842/148‑1 (MQ=255) tcaaaGCGGGGAGCGTACCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCttt > 4:169973/4‑145 (MQ=255) tcaaaGCGGGGAGCGTACCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCttt < 3:169973/142‑1 (MQ=255) tcaaaGCGGGGAGCGTACCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCTTTGGAc > 8:96067/4‑149 (MQ=255) ggggTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCTTTGGACCTTGCCa < 5:52907/149‑1 (MQ=255) gggTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTCCCACCCAATCATCAAAGGTTTCGAGAGAATACCACGCACGCCTGAGTAGCTCTTTGGACCTTGc > 7:215317/1‑146 (MQ=255) gCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCTTTGGACCTTGCCAGAGCTAg > 2:263727/1‑149 (MQ=255) cTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCTTTGGACCTTGCCAGAGCTAGt < 5:89750/149‑1 (MQ=255) | GGCGGCAAGCGAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCTTTGGACCTTGCCAGAGCTAGT > NC_002947/4496533‑4496819 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |