Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,516,189 C→G 9.1% S18R (AGC→AGG)  ate → arginyl‑tRNA‑‑protein transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,516,1890CG 9.1% 121.7 / 3.9 44S18R (AGC→AGG) atearginyl‑tRNA‑‑protein transferase
Reads supporting (aligned to +/- strand):  ref base C (23/17);  new base G (2/2);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

CCAGCCCAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTGCAACG  >  NC_002947/4516049‑4516331
                                                                                                                                             |                                                                                                                                              
ccAGCCCAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTAc                                                                                                                                             >  8:69176/1‑144 (MQ=255)
ccAGCCCAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTAc                                                                                                                                             <  7:69176/144‑1 (MQ=255)
   gCCCAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCg                                                                                                                                       >  2:57586/1‑147 (MQ=255)
       aaCAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGAGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACATGCCGgaggag                                                                                                                                 >  4:279582/1‑149 (MQ=255)
         cAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTTCGGCAGGTAGCTGCAggagtgggg                                                                                                                               <  8:136701/149‑6 (MQ=255)
               ttCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCg                                                                                                                           <  4:215611/147‑1 (MQ=255)
               ttCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCg                                                                                                                           >  3:215611/1‑147 (MQ=255)
                 cATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCa                                                                                                                       >  3:318988/1‑149 (MQ=255)
                  aTGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCa                                                                                                                       <  6:111933/148‑1 (MQ=255)
                      gTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTg                                                                                                                  <  3:11760/149‑1 (MQ=255)
                           cTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTcc                                                                                                              >  2:312146/1‑149 (MQ=255)
                            tAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGACGGAGGAGCAGGCGACCACGCTGTTCCTc                                                                                                            >  8:275665/1‑149 (MQ=255)
                              ggCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTc                                                                                                            <  6:284884/148‑1 (MQ=255)
                              ggCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGa                                                                                                          <  4:318988/149‑1 (MQ=255)
                                  aGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGc                                                                                                                                <  3:152201/123‑1 (MQ=255)
                                  aGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGc                                                                                                                                >  4:152201/1‑123 (MQ=255)
                                            gCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCggagga                                                                                                                                  <  8:290482/111‑1 (MQ=255)
                                            gCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCggagga                                                                                                                                  >  7:290482/1‑111 (MQ=255)
                                                  ttACACAA‑AAAAAAAACTCTCACCAAACGGGTTGATCATGACACACTTCCCGCGCTTGAAGATCTATTCCACTCACACACACTCATGCAGCAACCTGCCCGAGGAGCAGCCGACCACGCTGTTCCTCGACCCCACCCAGCCGATCGACg                                                                                      >  4:106627/1‑149 (MQ=255)
                                                      acTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTACTGCAGCTACCTGCAGGAGGAGCAGGCGACCCCGCTGTTCCTCGACCCCGGCCAGCCGATGGACGTGCa                                                                                  >  7:135921/1‑149 (MQ=255)
                                                        tt‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACg                                                                                >  1:328621/1‑149 (MQ=255)
                                                        tt‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACg                                                                                >  2:34809/1‑149 (MQ=255)
                                                        tt‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACg                                                                                >  8:68894/1‑149 (MQ=255)
                                                          aaaaaaaaaGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACg                                                                                <  1:312146/147‑1 (MQ=255)
                                                                    tctcACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGAt                                                                     >  2:261350/1‑149 (MQ=255)
                                                                    tctcACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACATGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGAt                                                                     >  4:252313/1‑149 (MQ=255)
                                                                         ccGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTc                                                                >  7:29410/1‑149 (MQ=255)
                                                                          cGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCg                                                               >  1:147046/1‑149 (MQ=255)
                                                                                 gTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATgg                                                        <  7:139409/149‑1 (MQ=255)
                                                                                 gTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATgg                                                        >  1:303020/1‑149 (MQ=255)
                                                                                  ttGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATggg                                                       <  1:57586/149‑1 (MQ=255)
                                                                                         tGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTcgc                                                <  2:147046/149‑1 (MQ=255)
                                                                                             agagTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCa                                            <  2:328621/149‑1 (MQ=255)
                                                                                                 ttGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTg                                         <  2:303020/148‑1 (MQ=255)
                                                                                                    gcgcGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGa                                     <  7:68894/149‑1 (MQ=255)
                                                                                                      gcgGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTACGCCGCAGTGGCGac                                    >  3:199305/1‑148 (MQ=255)
                                                                                                           tGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGc                                       <  6:75925/140‑1 (MQ=255)
                                                                                                           tGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGc                                       >  5:75925/1‑140 (MQ=255)
                                                                                                                               ccccACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGaa          <  3:279582/149‑1 (MQ=255)
                                                                                                                               ccccACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGaa          >  1:76246/1‑149 (MQ=255)
                                                                                                                                    cTCCGGCAGGTAGCTGCAGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTg                >  4:241676/1‑138 (MQ=255)
                                                                                                                                    cTCCGGCAGGTAGCTGCAGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTg                <  3:241676/138‑1 (MQ=255)
                                                                                                                                     tCCGGCAGGTATCTGCAGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGACAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGTGCTTTCGCCTCAGTGGCGACCACCTTTACCGCCCAAACTGCCAGAACTGCa     >  8:39071/1‑148 (MQ=255)
                                                                                                                                       cTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTGCAACg  >  6:37076/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                              
CCAGCCCAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTGCAACG  >  NC_002947/4516049‑4516331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑